Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Substrates Promotes Neural Priming

Eftekhari, Behnaz Sadat; Song, Dawei; Janmey, Paul A.

doi:10.1002/mabi.202300149

Cited by 3 publications

(2 citation statements)

References 93 publications

(74 reference statements)

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“…Several studies have demonstrated that neuronal differentiation, both of MSCs and NSCs, can be achieved by electrical stimulation [5,[28][29][30]; as well as it is possible to influence the neural lineage of differentiated NSCs through the electrical stimulation [7]. Responsible for the response to electrical stimulation are the voltage-gated calcium channels [29,31].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation

Innamorati,

Sanchez-Petidier,

Bergafora

et al. 2023

Preprint

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In the tissue regeneration field, stem cell transplantation and the control of their differentiation represent a promising therapeutic strategy. The second aspect can be achieved using electrical stimulation. This study aims to characterize the effect of a microsecond electrical stimulation on MSCs and iNSCs, in the context of the RISEUP FET-OPEN project (n. 964562) that seeks to control these cells differentiation for the spinal cord injury treatments. Here, the effect of a specific microsecond electric pulses stimulation, characterized by bipolar pulses of 100 µs + 100 µs, delivered for 30 minutes at an intensity of 250 V/cm, on i) cell proliferation, ii) cell cycle, iii) gene expression and iv) apoptosis was evaluated. Results show that the stimulation does not affect cell proliferation, cell cycle, and apoptosis, but induced some variations in gene expression, in particular in EGR1, FOS, and POU5F1. These ob-servations led us to deeply investigate the cell proliferation until 72h from the stimulation observing an increase in the iNSCs. The main outcome of this study is that the chosen stimulation protocol is safe and not toxic for MSCs and iNSCs. The observed variations in the gene expression need to be deeply investigated to assess the molecular mechanisms involved.

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Section: Discussionmentioning

confidence: 99%

Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation

Innamorati,

Sanchez-Petidier,

Bergafora

et al. 2023

Preprint

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show abstract

“…Electrical stimulation protocol conditions MSCs to enhance their proliferation, migration, differentiation, and scaffold adherence[ 20 ]. The effect of physiological level electrical stimulation has been shown to impact slowly; three days of culture has little effect compared to seven days and fourteen days to stimulate osteogenic, chondrogenic, and neural differentiation of the MSCs[ 21 , 22 ]. Similarly, the impact of electric stimulation is also determined by whether it is continuous or pulsed.…”

Section: Pretreatment Approaches Of Mscsmentioning

confidence: 99%

Priming mesenchymal stem cells to develop “super stem cells”

Haider

2024

World J Stem Cells

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The stem cell pre-treatment approaches at cellular and sub-cellular levels encompass physical manipulation of stem cells to growth factor treatment, genetic manipulation, and chemical and pharmacological treatment, each strategy having advantages and limitations. Most of these pre-treatment protocols are non-combinative. This editorial is a continuum of Li et al ’s published article and Wan et al ’s editorial focusing on the significance of pre-treatment strategies to enhance their stemness, immunoregulatory, and immunosuppressive properties. They have elaborated on the intricacies of the combinative pre-treatment protocol using pro-inflammatory cytokines and hypoxia. Applying a well-defined multi-pronged combinatorial strategy of mesenchymal stem cells (MSCs), pre-treatment based on the mechanistic understanding is expected to develop “Super MSCs”, which will create a transformative shift in MSC-based therapies in clinical settings, potentially revolutionizing the field. Once optimized, the standardized protocols may be used with slight modifications to pre-treat different stem cells to develop “super stem cells” with augmented stemness, functionality, and reparability for diverse clinical applications with better outcomes.

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The Roles of Micro‐ and Nanoscale Materials in Cell‐Engineering Systems

Jiang,

Harberts,

Assadi

et al. 2024

Advanced Materials

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Customizable manufacturing of ex vivo cell engineering is driven by the need for innovations in the biomedical field and holds substantial potential for addressing current therapeutic challenges; but it is still only in its infancy. Micro‐ and nanoscale‐engineered materials are increasingly used to control core cell‐level functions in cellular engineering. By reprogramming or redirecting targeted cells for extremely precise functions, these advanced materials offer new possibilities. This influences the modularity of cell reprogramming and reengineering, making these materials part of versatile and emerging technologies. Here, the roles of micro‐ and nanoscale materials in cell engineering are highlighted, demonstrating how they can be adaptively controlled to regulate cellular reprogramming and core cell‐level functions, including differentiation, proliferation, adhesion, user‐defined gene expression, and epigenetic changes. The current reprogramming routes used to achieve pluripotency from somatic cells and the significant potential of induced pluripotent stem cell technology for translational biomedical research are covered. Recent advances in nonviral intracellular delivery modalities for cell reprogramming and their constraints are evaluated. This paper focuses on emerging physical and combinatorial approaches of intracellular delivery for cell engineering, revealing the capabilities and limitations of these routes. It is showcased how these programmable materials are continually being explored as customizable tools for inducing biophysical stimulation. Harnessing the power of micro‐ and nanoscale‐engineered materials will be a step change in the design of cell engineering, producing a suite of powerful tools for addressing potential future challenges in therapeutic cell engineering.

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Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Substrates Promotes Neural Priming

Cited by 3 publications

References 93 publications

Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation

Characterization of Stem Cells Response to Bipolar Microsecond Pulses Electric Stimulation

Priming mesenchymal stem cells to develop “super stem cells”

The Roles of Micro‐ and Nanoscale Materials in Cell‐Engineering Systems

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