Electrical stimulation of human neural stem cells via conductive polymer nerve guides enhances peripheral nerve recovery

Enhanced sciatic nerve regeneration by relieving iron-overloading and organelle stress with the nanofibrous P(MMD-co-LA)/DFO conduits

“…Explants, which is the nerves cultured in vitro , undergo a process similar to Wallerian degeneration [ 29 , 30 ]. Explant cultures were used to detect iron overload during WD.…”

Mesoporous hollow Fe3O4 nanoparticles regulate the behavior of neuro-associated cells through induction of macrophage polarization in an alternating magnetic field

“…We wanted to explore the regulation of MHFPs on macrophage polarization, and at the same time examine the effect of macrophage polarization on neuronassociated cells. We selected two types of cells that play an important role in nerve regeneration: one is neural stem cells (NE-4C cells) that can directly differentiate into neurons and play an important role in promoting nerve repair; [29][30][31] the other is human vascular endothelial cells (HUVECs) that can form blood vessels to guide Schwann cell migration and neuron regeneration. 32 The effect of conditioned culture was reflected in the changes we noted in cell proliferation and migration, which may be more quantifiable, intuitive, and easier to test than other biological indicators.…”

Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

“…The following cells have been shown to need communication and interaction with extracellular matrix (ECM) proteins, which not only serve as structural components but also guide and support cell fate, adhesion, proliferation, survival and differentiation: 1 (a) human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs); 2–6 (b) adult stem cells such as neural progenitor stem cells (NPSCs), 7–9 bone marrow stem cells (BMSCs), 10–16 adipose-derived stem cells (ADSCs), 17–20 Wharton's Jelly-derived stem cells, 21–24 amniotic fluid stem cells 25–28 and dental pulp stem cells; 29–32 and (c) primary cells or differentiated stem cells such as epithelial cells, 33–35 endothelial cells, 36,37 neurons, 38–40 oligodendrocytes, 41–44 retinal pigment cells, 45–47 β cells 48–52 and hepatocytes. 53–57 The binding of the cells with ECM proteins via cell adhesion receptors such as integrins activates several signaling pathways that determine cell fate, morphological changes, proliferation and differentiation.…”