Electrically conductive gold nanoparticle-chitosan thermosensitive hydrogels for cardiac tissue engineering

Baei, Payam; Baharvand, Hossein; Rajabi-Zeleti, Sareh; Tafazzoli-Shadpour, Mohammad; Aghdami, Nasser

doi:10.1016/j.msec.2016.02.056

Cited by 289 publications

(226 citation statements)

References 52 publications

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“…During the preparation of levofloxacin chitosan microspheres via the ionic gelation method in the present study, levofloxacin molecules become surrounded by a network structure formed by chitosan and sodium tripolyphosphate (6,21,22). The particle size of the microspheres is in a range that meets the requirements of an ophthalmic particle dispersion system.…”

Section: Discussionmentioning

confidence: 91%

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Kong

Zhang

et al. 2017

Exp Ther Med

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In the present study, a temperature-sensitive gel composed of chitosan, carboxymethyl chitosan and glycerophosphate was prepared and loaded with chitosan microspheres encapsulating levofloxacin. The bioavailability of levofloxacin and the safety of this novel opthalmic drug delivery formulation were evaluated. Levofloxacin chitosan microspheres were prepared using the ionic gelation method, and the particle size and entrapment rate were determined. The morphology of the microspheres was observed by scanning electron microscopy. The pH and zeta potential were measured. The in vitro release of levofloxacin by the chitosan temperature-sensitive gel loaded with drug microspheres was determined using spectrophotometry. The eye retention time of the chitosan temperature-sensitive gel was calculated using a fluorescein sodium test. To assess the bioavailability and safety of the chitosan temperature-sensitive gel, a cell compatibility test, a cytotoxicity test and skin irritation test were performed. The entrapment rate of levofloxacin in the chitosan microspheres was determined to be 26.5%. The levofloxacin chitosan microspheres that were formed by chitosan and sodium tripolyphosphate were identified to be suitable for use in an ophthalmic particle dispersion system based on their physical and chemical properties. The pH of the levofloxacin chitosan microsphere suspension was 5.87±0.04, the average particle diameter was 2,452±342 nm, the polydispersity index was 0.168±0.028 and the ζ potential was 28.62±1.7 mV. The chitosan temperature-sensitive gel carrying microspheres loaded with drug prevented drug burst release at the initial stage and facilitated the slow release of the drug later on. Furthermore, this delivery system markedly prolonged the contact duration of levofloxacin with the eye. The chitosan temperature-sensitive hydrogel was safe and provided a good bioavailability of the drug. The results revealed that the chitosan temperature-sensitive gel had a cytotoxicity of grade 0, and no erythematous response was observed during the entire course of the skin irritation test. The present study provided a basis for the future development of the chitosan-based temperature-sensitive hydrogel in ophthalmic drug delivery.

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Section: Discussionmentioning

confidence: 91%

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Kong

Zhang

et al. 2017

Exp Ther Med

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“…In addition, ECHs have been extensively used as tissue engineered scaffolds to promote cellular adhesion, proliferation, differentiation, and growth, as well as the electroactive modulation of different cell types, including neurons, cardiomyocytes (CMs), cardiac fibroblasts (CFs), preosteoblasts, endothelial cells, and mesenchymal stem cells (MSCs) 18–23 . As a materials platform for tissue engineering, the physical properties of ECHs, such as their mechanical properties, water uptake capability, porosity, and degradation rate can influence tissue regeneration in vivo 24, 25 .…”

Section: Introductionmentioning

confidence: 99%

“…As a materials platform for tissue engineering, the physical properties of ECHs, such as their mechanical properties, water uptake capability, porosity, and degradation rate can influence tissue regeneration in vivo 24, 25 . In addition, excessive water uptake has been shown to impair the conductivity of ECHs, due to the percolation of fluids across the polymer network 23 . Therefore, several approaches have been used to modulate the conductivity and physical properties of ECHs, including the incorporation of different base polymers, bioactive components, porogens, and hydrophilic or hydrophobic moieties, as well as variations on the degree of crosslinking within the polymer networks 26–28 .…”

Section: Introductionmentioning

confidence: 99%

Engineering Biodegradable and Biocompatible Bio-ionic Liquid Conjugated Hydrogels with Tunable Conductivity and Mechanical Properties

Noshadi

Walker

Portillo-Lara

et al. 2017

Sci Rep

125

109

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Conventional methods to engineer electroconductive hydrogels (ECHs) through the incorporation of conductive nanomaterials and polymers exhibit major technical limitations. These are mainly associated with the cytotoxicity, as well as poor solubility, processability, and biodegradability of their components. Here, we describe the engineering of a new class of ECHs through the functionalization of non-conductive polymers with a conductive choline-based bio-ionic liquid (Bio-IL). Bio-IL conjugated hydrogels exhibited a wide range of highly tunable physical properties, remarkable in vitro and in vivo biocompatibility, and high electrical conductivity without the need for additional conductive components. The engineered hydrogels could support the growth and function of primary cardiomyocytes in both two dimentinal (2D) and three dimensional (3D) cultures in vitro. Furthermore, they were shown to be efficiently biodegraded and possess low immunogenicity when implanted subcutaneously in rats. Taken together, our results suggest that Bio-IL conjugated hydrogels could be implemented and readily tailored to different biomedical and tissue engineering applications.

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“…However, this study did not report cardiac injection data. Baei et al incorporated gold nanoparticles into chitosan to generate electroactive hydrogels (Figure 6B) [82], which represents a facile strategy of adding conductive particulates (e.g. metallic particles, carbon nanotubes, graphene nanosheets) to nonconductive hydrogel substrates to create conductive injectable biomaterials.…”

Section: New Strategies In Materials Designmentioning

confidence: 99%

Ventricular wall biomaterial injection therapy after myocardial infarction: Advances in material design, mechanistic insight and early clinical experiences

2017

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Intramyocardial biomaterial injection therapy for myocardial infarction has made significant progress since concept initiation more than 10 years ago. The interim successes and progress in the first 5 years have been extensively reviewed. During the last 5 years, two phase II clinical trials have reported their long term follow up results and many additional biomaterial candidates have reached preclinical and clinical testing. Also in recent years deeper investigations into the mechanisms behind the beneficial effects associated with biomaterial injection therapy have been pursued, and a variety of process and material parameters have been evaluated for their impact on therapeutic outcomes. This review explores the advances made in this biomaterial-centered approach to ischemic cardiomyopathy and discusses potential future research directions as this therapy seeks to positively impact patients suffering from one of the world’s most common sources of mortality.

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Electrically conductive gold nanoparticle-chitosan thermosensitive hydrogels for cardiac tissue engineering

Cited by 289 publications

References 52 publications

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Chitosan temperature‑sensitive gel loaded with drug microspheres has excellent effectiveness, biocompatibility and safety as an ophthalmic drug delivery system

Engineering Biodegradable and Biocompatible Bio-ionic Liquid Conjugated Hydrogels with Tunable Conductivity and Mechanical Properties

Ventricular wall biomaterial injection therapy after myocardial infarction: Advances in material design, mechanistic insight and early clinical experiences

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