Electrically Conductive Hydrogels for Articular Cartilage Tissue Engineering

Miguel, Filipe; Barbosa, Frederico; Ferreira, Frederico Castelo; Silva, João Carlos

doi:10.3390/gels8110710

Cited by 10 publications

(7 citation statements)

References 151 publications

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“…Among these, the use of conductive materials to make hydrogels has become a promising approach in tissue engineering. Even though the use of conductive materials has been investigated more in heart, skin, and nerve tissue engineering studies, nevertheless, in recent years, the use of these materials in cartilage tissue engineering has also attracted the attention of researchers ( Gao et al, 2022 ; Miguel et al, 2022 ). These types of materials have appeared valuable for cartilage tissue engineering applications due to their common biophysical properties, including greater simulation of the physiological environment and electrical stimulation ( Lee et al, 2022 ).…”

Section: Electroconductive Hydrogels For Cartilage Tissue Engineeringmentioning

confidence: 99%

A review of advanced hydrogels for cartilage tissue engineering

Ansari,

Darvishi,

Sabzevari

2024

Front. Bioeng. Biotechnol.

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With the increase in weight and age of the population, the consumption of tobacco, inappropriate foods, and the reduction of sports activities in recent years, bone and joint diseases such as osteoarthritis (OA) have become more common in the world. From the past until now, various treatment strategies (e.g., microfracture treatment, Autologous Chondrocyte Implantation (ACI), and Mosaicplasty) have been investigated and studied for the prevention and treatment of this disease. However, these methods face problems such as being invasive, not fully repairing the tissue, and damaging the surrounding tissues. Tissue engineering, including cartilage tissue engineering, is one of the minimally invasive, innovative, and effective methods for the treatment and regeneration of damaged cartilage, which has attracted the attention of scientists in the fields of medicine and biomaterials engineering in the past several years. Hydrogels of different types with diverse properties have become desirable candidates for engineering and treating cartilage tissue. They can cover most of the shortcomings of other treatment methods and cause the least secondary damage to the patient. Besides using hydrogels as an ideal strategy, new drug delivery and treatment methods, such as targeted drug delivery and treatment through mechanical signaling, have been studied as interesting strategies. In this study, we review and discuss various types of hydrogels, biomaterials used for hydrogel manufacturing, cartilage-targeting drug delivery, and mechanosignaling as modern strategies for cartilage treatment.

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Section: Electroconductive Hydrogels For Cartilage Tissue Engineeringmentioning

confidence: 99%

A review of advanced hydrogels for cartilage tissue engineering

Ansari,

Darvishi,

Sabzevari

2024

Front. Bioeng. Biotechnol.

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“…There are hypotheses that electric fields and fluid shear stress activate similar signaling pathways that involve integrin receptors [ 37 ]. One of the main cell responses to ES is the opening of voltage-gated calcium channels (VGCC) and subsequent increase of calcium (Ca 2+ ) inside the cell [ 38 ]. Both chondrocytes and mesenchymal stem cells have VGCC that can be regulated by external chemical or physical stimuli [ 39 , 40 ].…”

Section: Electrical Stimulation Overviewmentioning

confidence: 99%

“…ES was mostly applied on hydrogels or scaffolds containing natural cartilage ECM components (collagen, elastin, or hyaluronic acid) [ 45 , 101 , 102 ]; however, some studies have been performed with synthetic scaffolds [ 103 ]. Both biological and synthetic scaffolds have their own advantages, as natural polymer structures are more biocompatible while synthetic counterparts are mechanically stronger and easier to produce and manipulate [ 38 ], which makes it possible to improve conductive properties of the scaffold by, for example, incorporating conductive materials. Moreover, different scaffold manufacturing methods such as 3D printing and electrospinning result in scaffolds exhibiting varying properties (porosity, cell adhesion, etc.)…”

Section: The Effects Of Es On Chondrogenesis In Vitromentioning

confidence: 99%

“…Another important aspect of studies using ES on engineered cartilage tissue is the selection of scaffolds as they can both affect the strength of ES exerted on the cells as well as influence cell viability and chondrogenesis. Properties such as mechanical strength, conductivity and biocompatibility of the scaffolding material should be evaluated in the context of the experiment [ 38 ]. Furthermore, other scaffold parameters, such as porosity, and the introduction of parallel mechanical stimulation could play a role in the outcomes of ES studies.…”

Section: Recommendations On Reporting Es In the Context Of Chondrogen...mentioning

confidence: 99%

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Electrical Stimulation in Cartilage Tissue Engineering

et al. 2023

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Electrical stimulation (ES) has been frequently used in different biomedical applications both in vitro and in vivo. Numerous studies have demonstrated positive effects of ES on cellular functions, including metabolism, proliferation, and differentiation. The application of ES to cartilage tissue for increasing extracellular matrix formation is of interest, as cartilage is not able to restore its lesions owing to its avascular nature and lack of cells. Various ES approaches have been used to stimulate chondrogenic differentiation in chondrocytes and stem cells; however, there is a huge gap in systematizing ES protocols used for chondrogenic differentiation of cells. This review focuses on the application of ES for chondrocyte and mesenchymal stem cell chondrogenesis for cartilage tissue regeneration. The effects of different types of ES on cellular functions and chondrogenic differentiation are reviewed, systematically providing ES protocols and their advantageous effects. Moreover, cartilage 3D modeling using cells in scaffolds/hydrogels under ES are observed, and recommendations on reporting about the use of ES in different studies are provided to ensure adequate consolidation of knowledge in the area of ES. This review brings novel insights into the further application of ES in in vitro studies, which are promising for further cartilage repair techniques.

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“…Various conductive biomaterials have been applied for developing electroconductive scaffolds in the context of BTE. These materials can be essentially categorized into metals, carbon allotropes and conductive polymers [23][24][25]. The excellent conductivity of both metals and different carbon-based allotropes has led to their increasing use in BTE.…”

Section: Introductionmentioning

confidence: 99%

Novel Electroactive Mineralized Polyacrylonitrile/PEDOT:PSS Electrospun Nanofibers for Bone Repair Applications

Barbosa,

Garrudo,

Marques

et al. 2023

IJMS

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Bone defect repair remains a critical challenge in current orthopedic clinical practice, as the available therapeutic strategies only offer suboptimal outcomes. Therefore, bone tissue engineering (BTE) approaches, involving the development of biomimetic implantable scaffolds combined with osteoprogenitor cells and native-like physical stimuli, are gaining widespread interest. Electrical stimulation (ES)-based therapies have been found to actively promote bone growth and osteogenesis in both in vivo and in vitro settings. Thus, the combination of electroactive scaffolds comprising conductive biomaterials and ES holds significant promise in improving the effectiveness of BTE for clinical applications. The aim of this study was to develop electroconductive polyacrylonitrile/poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PAN/PEDOT:PSS) nanofibers via electrospinning, which are capable of emulating the native tissue’s fibrous extracellular matrix (ECM) and providing a platform for the delivery of exogenous ES. The resulting nanofibers were successfully functionalized with apatite-like structures to mimic the inorganic phase of the bone ECM. The conductive electrospun scaffolds presented nanoscale fiber diameters akin to those of collagen fibrils and displayed bone-like conductivity. PEDOT:PSS incorporation was shown to significantly promote scaffold mineralization in vitro. The mineralized electroconductive nanofibers demonstrated improved biological performance as observed by the significantly enhanced proliferation of both human osteoblast-like MG-63 cells and human bone marrow-derived mesenchymal stem/stromal cells (hBM-MSCs). Moreover, mineralized PAN/PEDOT:PSS nanofibers up-regulated bone marker genes expression levels of hBM-MSCs undergoing osteogenic differentiation, highlighting their potential as electroactive biomimetic BTE scaffolds for innovative bone defect repair strategies.

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Electrically Conductive Hydrogels for Articular Cartilage Tissue Engineering

Cited by 10 publications

References 151 publications

A review of advanced hydrogels for cartilage tissue engineering

A review of advanced hydrogels for cartilage tissue engineering

Electrical Stimulation in Cartilage Tissue Engineering

Novel Electroactive Mineralized Polyacrylonitrile/PEDOT:PSS Electrospun Nanofibers for Bone Repair Applications

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