ELECTRICALLY DRIVEN SEPARATION PROCESSES: ANALYTICAL AND PREPARATIVE METHODS<sup>*</sup>

Ivory, Cornelius F.

doi:10.1081/spm-100108161

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2008

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“…In order to achieve the required scale-up, scientists and engineers resort to flowing methods (2). Scale-up using electrophoresis is hindered by ohmic heating, which denatures the labile bio-molecules or cells.…”

Section: Introductionmentioning

confidence: 99%

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Electrophoretic Extraction of Cells/Particles in a Counter Current Extractor

Chethana

Patil²,

Madhusudhan

et al. 2008

Separation Science and Technology

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Electrophoretic separation is a leading method for resolving mixtures of charged macromolecules or cells. In the present work, a combination of free electrophoresis and multistage extraction called Electrophoretic Counter Current Extractor was explored. A simple mathematical model was developed to describe the mass and heat transfer during the electrophoretic separation. In order to validate the model and also to test the extractor, the extraction of fixed human Red Blood Cells and Latex particles of different sizes suspended in phosphate buffer, was performed at different electric field strengths. The experimental results were found to agree reasonably well with those predicted by the model.

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Section: Introductionmentioning

confidence: 99%

“…Electrophoretic separation of proteins without gels (free electrophoresis) has been a long-standing goal of separation research (1,2). These separations are influenced either separately or jointly by factors such as the size (or molecular weight), shape, secondary structure, density, and charge of the macromolecule or cell.…”

Section: Introductionmentioning

confidence: 99%