Electrically insulating pore-suspending membranes on highly ordered porous alumina obtained from vesicle spreading

Schmitt, Eva K.; Nurnabi, Mohammad; Bushby, Richard J.; Steinem, Claudia

doi:10.1039/b716723g

Cited by 62 publications

(57 citation statements)

References 22 publications

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“…The formation of nano-BLMs as well as their electrical characteristics were investigated by impedance analysis using the gain/phase analyzer SI 1260 and the 1296 Dielectric Interface (Solartron Instruments, Farnborough, UK) as described previously (Schmitt et al, 2008). Platinized platinum wires served as working and counter electrodes.…”

Section: Methodsmentioning

confidence: 99%

The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

Gaßmann

Kreir

Ambrosi

et al. 2009

Journal of Structural Biology

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Connexin26 (Cx26) is a member of the connexin family, the building blocks for gap junction intercellular channels. These dodecameric assemblies are involved in gap junction-mediated cellcell communication allowing the passage of ions and small molecules between two neighboring cells. Mutations in Cx26 lead to the disruption of gap junction-mediated intercellular communication with consequences such as hearing loss and skin disorders. We show here that a mutant of Cx26, M34A, forms an active hemichannel in lipid bilayer experiments. A comparison with the Cx26 wild-type is presented. Two different techniques using micro/nano-structured substrates for the formation of poresuspending lipid membranes are used. We reconstituted the Cx26 wild-type and Cx26M34A into artificial lipid bilayers and observed single channel activity for each technique, with conductance levels of around 35, 70 and 165 pS for the wildtype. The conductance levels of Cx26M34A were found at around 45 and 70 pS.

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Section: Methodsmentioning

confidence: 99%

The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

Gaßmann

Kreir

Ambrosi

et al. 2009

Journal of Structural Biology

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“…19–24 For example, Steinem and coworkers used nanoporous electrodes coated with planar bilayers to support the proton-pumping activity of incorporated bacteriorhodopsin. 25 These and other examples suggest the potential for “wiring” a planar membrane to substrate electrode to allow use of the transmembrane potential gradient to drive a coupled electrochemical process.…”

Section: Introductionmentioning

confidence: 99%

Poly(aniline) Nanowires in Sol–Gel Coated ITO: A pH-Responsive Substrate for Planar Supported Lipid Bilayers

Orosz

Armstrong

et al. 2011

ACS Appl. Mater. Interfaces

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Facilitated ion transport across an artificial lipid bilayer coupled to a solid substrate is a function common to several types of bioelectronic devices based on supported membranes, including biomimetic fuel cells and ion channel biosensors. Described here is fabrication of a pH-sensitive transducer composed of a porous sol-gel layer derivatized with poly(aniline) (PANI) nanowires grown from an underlying planar indium-tin oxide (ITO) electrode. The upper sol-gel surface is hydrophilic, smooth, and compatible with deposition of a planar supported lipid bilayer (PSLB) formed via vesicle fusion. Conducting tip AFM was used to show that the PANI wires are connected to the ITO, which convert this electrode into a potentiometric pH sensor. The response to changes in the pH of the buffer contacting the PANI nanowire/sol-gel/ITO electrode is blocked by the very low ion permeability of the overlying, fluid PSLB. The feasibility of using this assembly to monitor facilitated proton transport across the PSLB was demonstrated by doping the membrane with lipophilic ionophores that respond to a transmembrane pH gradient, which produced an apparent proton permeability several orders of magnitude greater than values measured for undoped lipid bilayers.

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“…Such phenomena have also been observed with an ingenious connection to create a polythiol containing 240 repeat units on AAO surface. Building up the concept of gold coated AAO surfaces, Steinem group [13][14][15] showed that the suspended lipid bilayers could be done on alkanethiols coated AAO supports with the ability to mimic biological membrane and monitor ion channel activities ( Fig. 5.2).…”

Section: Thiol Surface Chemistrymentioning

confidence: 99%

Soft and Hard Surface Manipulation of Nanoporous Anodic Aluminum Oxide (AAO)

et al. 2015

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Nanoporous materials with straight channels have attracted considerable interest due to their unique physical properties and many potential applications such as separation, sensing, biomedical and electronics. For the last few decades, nanoporous alumina or anodic aluminium oxide (AAO) membrane is gaining attention due to its broad applicability in various applications. The unique properties of AAO membrane coupled with tunable surface modification and properties is playing an increasingly important platform in a diverse range of applications such as separation, energy storage, drug delivery and template synthesis, as well as biosensing, tissue engineering and catalytic studies. This chapter aims to introduce the recent advances and challenges for surface manipulation of AAO following the 'soft' and 'hard' modification strategies. The functions of these modified nanostructures materials and latest important applications are evaluated with respect to improved performance and possible implications of those strategies for the future trends of surface engineering are discussed.

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Electrically insulating pore-suspending membranes on highly ordered porous alumina obtained from vesicle spreading

Cited by 62 publications

References 22 publications

The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

Poly(aniline) Nanowires in Sol–Gel Coated ITO: A pH-Responsive Substrate for Planar Supported Lipid Bilayers

Soft and Hard Surface Manipulation of Nanoporous Anodic Aluminum Oxide (AAO)

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