Electricity-driven bioproduction from CO2 and N2 feedstocks using enriched mixed microbial culture

Yadav, Ravineet; Chiranjeevi, P.; Yadav, Sukrampal; Singh, Ramandeep; Patil, Sunil A.

doi:10.1016/j.jcou.2022.101997

Cited by 7 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the first 214 days of enrichment, 0.6 to 3.3% of the electrons were used for N 2 fixation in the two PCE. In comparison, efficiencies of 0.5% and 20% for NH 4 + synthesis was reported in two recent works carried out under similar conditions (Yadav et al, 2022; L. Zhang et al, 2022). As the amount of fixed N was highly dependent on biomass accumulation, negative results were obtained at day 244 when biomass started to decrease.…”

Section: Resultsmentioning

confidence: 52%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

View full text Add to dashboard Cite

The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Low-energy bioelectrochemical fixation of N2to microbial biomass was previously observed on a biocathode but the microbial interactions in N2-fixing electroactive biofilms are still poorly understood. The present study aims to develop a method of enrichment of autotrophic and diazotrophic bacteria from soil samples for further characterization. The enrichment method was based on a first classical step of selection of N2-fixing bacteria from soil samples. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H2(hydrogenotrophic) or the cathode as energy sources. This enrichment was compared with an enrichment of diazotrophic hydrogenotrophic bacteria without the use of the microbial electrochemical system. Both methods showed comparable results for N2fixation rates at day 340 of the enrichment, with an estimated average of approximately 0.2 mgNfixed/L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment. These results confirm the enrichment of autotrophic, electrotrophic and diazotrophic bacteria in the polarized cathode enrichments. Finally, the analysis of the enriched communities suggest thatDesulforamulus ruminismediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N2fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N2-fixing electroactive biofilms was proposed.

show abstract

Section: Resultsmentioning

confidence: 52%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…During the first period (131-214 days) current density increased, whereas during the second period (>215) current density decreased after several power outages (see Figure 3). In comparison with the other works dealing with N2-fixing cathodic biofilms, Zhang et al (2022) showed a maximum of 40.5 mg/L of NH4 + in 4 days with mixed communities, in regard to a maximum of 0.8 mg/L NH4 + observed in Yadav et al (2022) and 6.31 mg/L NH4 + in 10 days for Li et al (Li et al, 2022;Yadav et al, 2022;Zhang et al, 2022). When demonstrating the N2 fixation in MES, Rago et al (2019) showed a N2 fixation of 0.2 mgN/L.d in biomass and 5 10 9 bacteria/L.d (Rago et al, 2019).…”

Section: N Quantification and Coulombic Efficiencymentioning

confidence: 46%

“…During the first 214 days of enrichment, 0.6 to 3.3% of the electrons were used for N2 fixation in the two PCE. In comparison, efficiencies of 0.5% and 20% for NH4 + synthesis was reported in two recent works carried out under similar conditions (Yadav et al, 2022;Zhang et al, 2022). As the amount of fixed N was highly dependent on biomass accumulation, negative results were obtained at day 244 when biomass started to decrease.…”

Section: N Quantification and Coulombic Efficiencymentioning

confidence: 61%

“…Other works investigated the mechanisms with pure bacterial strain like Soundararajan et al ( 2019) and Chen et al (2020) with Rhodopseudomonas palustris and Pseudomonas stutzeri (Chen et al, 2020;Soundararajan et al, 2019). Yadav et al (2022) demonstrated the possible use of N2-fixing bacteria as nitrogen source in a microbial electrosynthesis process of acetate (Yadav et al, 2022). Zhang et al (2022) also worked on a system of N2 fixation in microbial electrolysis cell (MEC) (Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Coupling capabilities of N2 fixation in bioelectrochemical systems as possible source of nitrogen for other biological systems was subject of interest. Indeed, such coupling can lead to the production of molecules of interest such as acetate by reducing the environmental impact of the use of reactive nitrogen often in the form of NH4Cl (Yadav et al, 2022). The work of Li et al (2022) in a single-chamber system and highlighted an importance of synergy within an N2-fixing community in a microbial bioremediation system (Li et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous,

Santa-Catalina,

Desmond-Le Quémener

et al. 2024

Peer Community Journal

View full text Add to dashboard Cite

The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Lowenergy bioelectrochemical fixation of N 2 to microbial biomass was previously observed but the mechanisms of microbial interactions in N 2 -fixing electroactive biofilms are still poorly understood. The present study aims to develop a new method of enrichment of autotrophic and diazotrophic bacteria from soil samples with a better electron source availability than using H 2 alone. The enrichment method was based on a multi-stage procedure. The first enrichment step was specifically designed for the selection of N 2 -fixing bacteria from soil samples with organic C as electron and carbon source. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H 2 (hydrogenotrophic) or the cathode as electron source. This enrichment was compared with an enrichment culture of pure diazotrophic hydrogenotrophic bacteria without the use of a microbial electrochemical system. Interestingly, both methods showed comparable results for N 2 fixation rates at day 340 of the enrichment with an estimated average of approximately 0.2 mgN fixed /L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment.These results confirmed an enrichment in autotrophic and diazotrophic bacteria on the polarized cathode. It was hypothesied that autotrophic bacteria were able to use either the H 2 produced at the cathode or directly the cathode through direct electron transfer (DET) as more biomass was produced than with H 2 alone. Finally, the analysis of the enriched communities suggested that Desulforamulus ruminis mediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N 2 fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N 2 -fixing electroactive biofilms was proposed.

show abstract

Advanced biological and non-biological technologies for carbon sequestration, wastewater treatment, and concurrent valuable recovery: A review

Kumar

Priyadarshini

Ahmad

et al. 2023

Journal of CO2 Utilization

View full text Add to dashboard Cite

Electricity-driven bioproduction from CO2 and N2 feedstocks using enriched mixed microbial culture

Cited by 7 publications

References 33 publications

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Advanced biological and non-biological technologies for carbon sequestration, wastewater treatment, and concurrent valuable recovery: A review

Contact Info

Product

Resources

About