2020
DOI: 10.3389/fncel.2020.00223
|View full text |Cite
|
Sign up to set email alerts
|

Electro-Acupuncture Promotes the Differentiation of Endogenous Neural Stem Cells via Exosomal microRNA 146b After Ischemic Stroke

Abstract: Background: Evidences indicate that exosomes-mediated delivery of microRNAs (miRNAs or miRs) is involved in the neurogenesis of stroke. This study was to investigate the role of exosomal miRNAs in non-drug therapy of electro-acupuncture (EA) regulating endogenous neural stem cells for stroke recovery. Methods: The model of focal cerebral ischemia and reperfusion in rats were established by middle cerebral artery occlusion (MCAO) and treated by EA. The exosomes were extracted from peri-ischemic striatum and ide… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
34
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 46 publications
(34 citation statements)
references
References 46 publications
0
34
0
Order By: Relevance
“…In addition, exosomal cargo, including miR-21, can regulate sensory neurons for macrophage communication after nerve trauma [ 42 ], indicating that exosomal miR-21 has the potential to mediate and treat PNI. Levels of the exosomal biomarkers, TSG101 and CD81, increased in the ischemic striatum after EA treatment [ 64 ], suggesting that EA may stimulate an increase in exosomes secreted. Therefore, the regulation of secretions and transfer of exosomes between cells may be one of the principal mechanisms of EA treatment.…”
Section: Discussionmentioning
confidence: 99%
“…In addition, exosomal cargo, including miR-21, can regulate sensory neurons for macrophage communication after nerve trauma [ 42 ], indicating that exosomal miR-21 has the potential to mediate and treat PNI. Levels of the exosomal biomarkers, TSG101 and CD81, increased in the ischemic striatum after EA treatment [ 64 ], suggesting that EA may stimulate an increase in exosomes secreted. Therefore, the regulation of secretions and transfer of exosomes between cells may be one of the principal mechanisms of EA treatment.…”
Section: Discussionmentioning
confidence: 99%
“…Total RNA was isolated from the ischemic cerebral tissues by using the Trizol method (Life Technologies, United States) ( 17 , 22 ). Thereafter, 1 μg of total RNA was reverse transcribed using a cDNA synthesis kit (N8050200, Life Technologies Holdings Pte Ltd.) according to the manufacturer's instructions, followed by real-time quantitative PCR (qRT-PCR) by using the SYBR kit.…”
Section: Methodsmentioning
confidence: 99%
“…EA not only offers strong and stable stimulation but also can be applied to multiple targets ( 11 , 12 ). Recent studies have shown that EA exhibits anti-apoptotic effects ( 13 ), inhibits autophagy ( 14 ), exhibits anti-inflammatory properties ( 15 ), reduces antioxidant stress ( 16 ), and promotes the proliferation and differentiation of neural stem cells ( 17 ), and neurovascular unit (NVU) remodeling ( 18 ). However, the mechanisms of action of EA in ischemic stroke are yet to be determined.…”
Section: Introductionmentioning
confidence: 99%
“…In addition, exosomes released by the neural cells are reported to regulate the development and progression of nervous system diseases but to also play an important role in regeneration and remodeling of the nervous system after neural injury (40). Zhang et al indicated that electroacupuncture enhanced the differentiation of endogenous neurogenesis and mitigated neurological deficits after ischemic stroke (41). Exosomal miR-146b is an important neuromodulator of neurogenesis that promotes endogenous neural stem cell differentiation into neurons in peri-ischemia after stroke.…”
Section: Promoting Neurogenesismentioning
confidence: 99%