Electro-acupuncture regulates glucose metabolism in chronic stress model rats

Abstract

Studies have shown that acupuncture is very effective in treating chronic stress depression. However, little is known about the therapeutic mechanism of electro-acupuncture. Metabolomics, on the other hand, is a technology that determines the metabolic changes of organisms caused by various interventions as a whole and is related to the overall effect of electro-acupuncture (EA). 1 HNMR, serum sample analysis, and histopathology and molecular biology analysis were used to evaluate the effects of EA. The results show that electro-acupuncture points can regulate the heat pain threshold of chronic stress model rats and change the morphology of adrenal cortex cells Structure, and regulate the contents of corticotropin-releasing hormone, Corticosterone (CORT), glucose, alanine and valine in the samples. These findings help to clarify the therapeutic mechanism of electro-acupuncture on heterologous chronic stress model rats. The effect of electro-acupuncture on improving chronic stress is likely to be achieved by regulating glucose metabolism, which can provide a reference for clinical acupuncture treatment of chronic stress depression. With the rapid development of the economy and society, everyone faces a stressful environment in daily life. Stress is a systemic, non-specific, adaptive response to any physiological or psychological stimulus that disrupts persistent homeostasis 1. The effect of stress on the body seems to depend on the duration, not the intensity of external or internal stress stimuli 2. The introduction of unpredictable factors makes it difficult for individuals to adapt to stressors. Therefore, chronic stress may induce and aggravate damage to tissues and organs 3. Chronic stress is an important risk factor for depression 4. Despite some findings about chronic stress, we still lack effective clinical treatments. Acupuncture is the most popular adjuvant and alternative therapy in China, and it has been used for thousands of years 5. Electro-acupuncture is an innovation of traditional Chinese acupuncture, which improves the clinical effect by transmitting electrical pulses to the needle 6. Studies show that acupuncture has been used as an alternative therapy for depression in clinical practice 7-9. Acupuncture can help reduce chronic stress-like behaviour by regulating hypothalamic-pituitary-adrenal (HPA) axis function 10. However, it cannot reflect the biological state and regulatory function of the entire human body after external stimulation. At present, there are few studies on chronic stress-induced dysfunction in EA, and empirical evidence is lacking. Therefore, it is important to study the effects of EA on the mechanism of chronic stress effects. Metabolomics can simultaneously monitor and evaluate changes in the metabolic spectrum caused by changes in disease and other stimuli in a holistic context. It can be characterised in a timely and sensitive manner 11,12. This provides a good technical means for us to study the mechanism of chronic stress and the intervention effect of electro-acupunctu...

O uso da acupuntura como prática complementar para o tratamento de pacientes com diabetes mellitus tipo 2: uma revisão integrativa da literatura

“…Além disso, o método de EA também pode ser utilizado para tratar a obesidade através de outros mecanismos, como a supressão da inflamação e a melhora do metabolismo lipídico. A técnica também pode contribuir para a melhora do quadro de sensibilidade à insulina, promove a perda de peso, possui efeito anti-inflamatório e atua no metabolismo lipídico e de adipocinas (Shapira et al, 2000;Ma et al, 2020).…”

Electroacupuncture Prevents the Depression-Like Behavior by Inhibiting the NF-κB/NLRP3 Inflammatory Pathway in Hippocampus of Mice Subjected to Chronic Mild Stress

“…An early study demonstrated that manual stimulation of the Kl 3 acupoint influenced significantly peripheral vascular circulation, by means of sympathetic stimulation causing vasoconstriction [30]. Acupuncture is widely used in clinical practice and had been shown to have antidepressant effects in both clinical and animal studies [7,8,[31][32][33].…”

“…Clinically, depressive patients prefer complementary therapies to drug therapy [6]. Acupuncture is widely used in clinical practice and had been shown to have antidepressant effects in both clinical and animal studies [7, 8]. Combined antidepressants and acupuncture therapy are more effective than drugs alone [7].…”

“…Electroacupuncture (EA) is defined as the application of a small current through acupuncture needles; it is thought to deliver a greater dose of acupuncture compared with manual acupuncture, as a result of the intensity and duration of stimulation [8]. EA is widely used in clinical practice, as well as in experimental investigations into the physiological mechanisms of acupuncture.…”

Effects of Electroacupuncture on Gastrointestinal Motility Function, Pain, and Inflammation via Transient Receptor Potential Vanilloid 1 in a Rat Model after Colonic Anastomoses

“…EA Treatment. ST36 is localized on 5 mm below the caput fibulae on the posterolateral side of the knee according to "The Veterinary Acupuncture of China" [13]. Animals in the EA group were fastened to a specially designed holder.…”