Electro-gene therapy of collagen-induced arthritis by using an expression plasmid for the soluble p75 tumor necrosis factor receptor-Fc fusion protein

Jm, Kim; Sh, Ho; Hahn, Woong; Jeong, Joo‐Won; Park, EJ; Hj, Lee; Yu, Shiying; Cs, Lee; Lee, YW; S, Kim

doi:10.1038/sj.gt.3301985

Cited by 49 publications

(18 citation statements)

References 45 publications

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“…Anti-inflammatory cytokine IL-10 showed interesting properties in an atherosclerosis model [60] and exhibited a protective role in a mouse model of rheumatoid arthritis [23]. In the same model of chronic inflammatory disease, electrotransfer of a soluble TNF-α receptor led to reduction of the signs of the disease [73].…”

Section: Intramuscular Electrotransfermentioning

confidence: 99%

“…This chimeric protein is bioactive as shown by electrotransfer of 50 µg of plasmid encoding this protein in a model of LPS systemic inflammation: a clear reduction in the amount of circulating TNF-α and IL-1β is observed (Figure 8). It has also proven efficient in collagen-induced arthritis on clinical and histological scores of the disease [73]. The same kind of protein was constructed by linking viral IL-10 gene to the non-cytolytic immunoglobulin Fc portion.…”

Section: Design Of Molecules For Electrotransfermentioning

confidence: 99%

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Plasmid DNA electrotransfer for intracellular and secreted proteins expression: new methodological developments and applications

et al. 2004

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SummaryIn vivo electrotransfer is a physical method of gene delivery in various tissues and organs, relying on the injection of a plasmid DNA followed by electric pulse delivery. The importance of the association between cell permeabilization and DNA electrophoresis for electrotransfer efficiency has been highlighted. In vivo electrotransfer is of special interest since it is the most efficient non-viral strategy of gene delivery and also because of its low cost, easiness of realization and safety. The potentiality of this technique can be further improved by optimizing plasmid biodistribution in the targeted organ, plasmid structure, and the design of the encoded protein. In particular, we found that plasmids of smaller size were electrotransferred more efficiently than large plasmids. It is also of importance to study and understand kinetic expression of the transgene, which can be very variable, depending on many factors including cellular localization of the protein, physiological activity and regulation. The most widely targeted tissue is skeletal muscle, because this strategy is not only promising for the treatment of muscle disorders, but also for the systemic secretion of therapeutic proteins. Vaccination and oncology gene therapy are also major fields of application of electrotransfer, whereas application to other organs such as liver, brain and cornea are expanding. Many published studies have shown that plasmid electrotransfer can lead to long-lasting therapeutic effects in various pathologies such as cancer, blood disorders, rheumatoid arthritis or muscle ischemia. DNA electrotransfer is also a powerful laboratory tool to study gene function in a given tissue.

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Section: Intramuscular Electrotransfermentioning

confidence: 99%

Section: Design Of Molecules For Electrotransfermentioning

confidence: 99%

Plasmid DNA electrotransfer for intracellular and secreted proteins expression: new methodological developments and applications

et al. 2004

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“…Gene transfer by in vivo electroporation has been shown to be effective in several animal models including arthritis [25][26][27][28][29]. We previously observed that intramuscular electrotransfer of plasmid expressing soluble TNF receptor (sTNFR : Fc) could exert beneficial effects on CIA in mice [29].…”

Section: Discussionmentioning

confidence: 98%

“…Intramuscular electrotransfer has been tested in several animal models [25]. In particular, recent reports have shown that experimental arthritis in animal models was ameliorated by electrotransfer of plasmid DNA encoding IL-10, TIMP-4 or soluble TNF receptor [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Electrotransfer of human IL‐1Ra into skeletal muscles reduces the incidence of murine collagen‐induced arthritis

Jeong

Kim

et al. 2004

The Journal of Gene Medicine

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“…The local injection of expression plasmids encoding immunosuppressive cytokines such as IL-4, TGF 1 and IL-10 has been shown to increase the level of these cytokines in circulation, enhance humoral and cellular immune responses and protect against several autoimmune diseases in animal models [242][243][244][245][246][247]. Antagonizing the cytokines with proinflammatory effects has also demonstrated to prevent progression of the disease in experimental autoimmune diseases such as arthritis, IDDM and SLE [248][249][250][251]. Also, ex vivo gene transfer of these cytokines or cytokine inhibitory proteins through modifying antigen-specific T-cells and fibroblasts has shown to be an effective therapy in induced forms of arthritis in animal models [252,253].…”

Section: N O T F O R D I S T R I B U T I O Nmentioning

confidence: 99%

Autoimmunity and Apoptosis - Therapeutic Implications

Rashedi

Panigrahi²,

Ezzati³

et al. 2007

CMC

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Acquisition of a complex immune system during evolution provided organisms with the most effective defense mechanism against "foreign" or "non-self" invaders. This efficient protection against pathogens, however, has been achieved at the expense of a higher risk for "self"-directed reaction or autoimmunity. Establishment of self-tolerance and homeostasis in the immune system is regulated at different physiological stages of immune cells development. The breakdown in discrimination between "self" and "non-self" causes an aberrant immune response against autoantigens that promote damage to the "self" cells and tissue(s), resulting in various autoimmune phenotypes. Whereas activation and clonal proliferation of autoreactive T-and B-lymphocytes underlies the pathogenesis of autoimmune diseases, the mechanism by which self-tolerance is lost and autoimmune responses are induced is not clear yet. Autoimmunity is a multi-step process that occurs as a consequence of complex interaction between genetic susceptibility and non-genetic factors. Programmed cell death, as a key mechanism to regulate immune system function, has a crucial influence on both the selection process of immune cells and the maintenance of this immune tolerance in peripheral repertoire. Thus, defects in apoptotic death pathways may contribute to the development of autoimmune response in susceptible individuals in certain conditions.

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Electro-gene therapy of collagen-induced arthritis by using an expression plasmid for the soluble p75 tumor necrosis factor receptor-Fc fusion protein

Cited by 49 publications

References 45 publications

Plasmid DNA electrotransfer for intracellular and secreted proteins expression: new methodological developments and applications

Plasmid DNA electrotransfer for intracellular and secreted proteins expression: new methodological developments and applications

Electrotransfer of human IL‐1Ra into skeletal muscles reduces the incidence of murine collagen‐induced arthritis

Autoimmunity and Apoptosis - Therapeutic Implications

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