Electro-therapies for sarcomas

Sundararajan, Raji; Poompavai, S.; Sree, Gowri; Madhivanan, S; Prabakaran, Sudhakaran; Camarillo, Ignacio G.

doi:10.15406/jcpcr.2019.10.00403

Cited by 7 publications

(9 citation statements)

References 30 publications

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“…Colloidal materials can be used as microscale bots capable of translation [1][2][3][4][5][6][7][8] and cargo delivery. [9][10][11][12][13][14] Methods to power and direct them include electric, 15 optical, 16 magnetic, [17][18][19] or chemical 20,21 gradients, self-propelling particles, [22][23][24] various motile structures including rotors, 25 microworms, 26 and swarms, 27,28 and particle geometries that break symmetry. [29][30][31] Our previous work has focused on the use of colloidal building blocks that can be assembled in situ using alternating current (ac) magnetic fields to create rotating microwheels (μwheels) that translate by rolling on nearby surfaces.…”

Section: Introductionmentioning

confidence: 99%

ac/dc Magnetic Fields for Enhanced Translation of Colloidal Microwheels

2019

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Microscale devices must overcome fluid reversibility to propel themselves in environments where viscous forces dominate. One approach, used by colloidal microwheels (μwheels) consisting of superparamagnetic particles assembled and powered by rotating ac magnetic fields, is to employ a nearby surface to provide friction. Here, we used total internal reflection microscopy to show that individual 8.3 μm particles roll inefficiently with significant slip because of a particle-surface fluid gap of 20-80 nm. We determined that both gap width and slip increase with the increasing particle rotation rate when the load force is provided by gravity alone, thus providing an upper bound on translational velocity. By imposing an additional load force with a dc magnetic field gradient superimposed on the ac field, we were able to decrease the gap width and thereby enhance translation velocities. For example, an additional load force of 0.2 F g provided by a dc field gradient increased the translational velocity from 40 to 80 μm/s for a 40 Hz rotation rate. The translation velocity increases with the decreasing gap width whether the gap is varied by dc field gradient-induced load forces or by reducing the Debye length with salt. These results present a strategy to accelerate surface-enabled rolling of microscale particles and open the possibility of high-speed μwheel rolling independent of the gravitational field.

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Section: Introductionmentioning

confidence: 99%

ac/dc Magnetic Fields for Enhanced Translation of Colloidal Microwheels

2019

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“…Taken together, the cancer cells are more susceptible to the electrical stimulation than the normal cells. The electric field is inversely proportional to distance between the electrode and tissues, and the proximity effect is negligible . These results suggest that Ni–Ti wire-based electrodes effectively deliver electrical energy to cancer cells through ion pumps and channels in the cell membrane pathway .…”

Section: Results and Discussionmentioning

confidence: 85%

“…The electric field is inversely proportional to distance between the electrode and tissues, and the proximity effect is negligible. 37 These results suggest that Ni−Ti wire-based electrodes effectively deliver electrical energy to cancer cells through ion pumps and channels in the cell membrane pathway. 38 In addition, selective cell death was directly generated in contact with the electrode; therefore, this IRE system is expected to provide a new therapeutic option to treat endoluminal tumor in the future.…”

Section: Cytotoxicity and Viability Using The Chemically Polished Ni−...mentioning

confidence: 92%

Self-Expandable Electrode Based on Chemically Polished Nickel–Titanium Alloy Wire for Treating Endoluminal Tumors Using Bipolar Irreversible Electroporation

Kim

Kang

Park

et al. 2023

ACS Appl. Mater. Interfaces

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The application of irreversible electroporation (IRE) to endoluminal organs is being investigated; however, the current preclinical evidence and optimized electrodes are insufficient for clinical translation. Here, a novel self-expandable electrode (SE) made of chemically polished nickel−titanium (Ni−Ti) alloy wire for endoluminal IRE is developed in this study. Chemically polished heat-treated Ni−Ti alloy wires demonstrate increased electrical conductivity, reduced carbon and oxygen levels, and good mechanical and self-expanding properties. Bipolar IRE using chemically polished Ni−Ti wires successfully induces cancer cell death. IRE-treated potato tissue shows irreversibly and reversibly electroporated areas containing dead cells in an electrical strengthdependent manner. In vivo study using an optimized electric field strength demonstrates that endobiliary IRE using the SE evenly induces well-distributed mucosal injuries in the common bile duct (CBD) with the overexpression of the TUNEL, HSP70, and inflammatory cells without ductal perforation or stricture formation. This study demonstrates the basic concept of the endobiliary IRE procedure, which is technically feasible and safe in a porcine CBD as a novel therapeutic strategy for malignant biliary obstruction. The SE is a promising electrical energy delivery platform for effectively treating endoluminal organs.

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“…IRE generally ablates tumor tissues by damaging the cellular membrane using the specific conditions of voltage (1000 V), duration (100 µs), and 8 pulses [14,40]. However, since voltages above 1000 V showed too much toxicity in vitro condition (data not shown), we evaluated the cancer cell death rate by fixing the voltage at 1000 V and changing the pulses.…”

Section: In Vitro Cellular Experiments To Establish Electrical Conditions For Irementioning

confidence: 99%

Combination of Irreversible Electroporation and STING Agonist for Effective Cancer Immunotherapy

Yang

Chon

et al. 2020

Cancers

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Recently, cancer immunotherapy has received attention as a viable solution for the treatment of refractory tumors. However, it still has clinical limitations in its treatment efficacy due to inter-patient tumor heterogeneity and immunosuppressive tumor microenvironment (TME). In this study, we demonstrated the triggering of anti-cancer immune responses by a combination of irreversible electroporation (IRE) and a stimulator of interferon genes (STING) agonist. Optimal electrical conditions inducing damage-associated molecular patterns (DAMPs) by immunogenic cell death (ICD) were determined through in vitro 2D and 3D cell experiments. In the in vivo syngeneic lung cancer model, the combination of IRE and STING agonists demonstrated significant tumor growth inhibition. We believe that the combination strategy of IRE and STING agonists has potential for effective cancer immunotherapy.

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Electro-therapies for sarcomas

Cited by 7 publications

References 30 publications

ac/dc Magnetic Fields for Enhanced Translation of Colloidal Microwheels

ac/dc Magnetic Fields for Enhanced Translation of Colloidal Microwheels

Self-Expandable Electrode Based on Chemically Polished Nickel–Titanium Alloy Wire for Treating Endoluminal Tumors Using Bipolar Irreversible Electroporation

Combination of Irreversible Electroporation and STING Agonist for Effective Cancer Immunotherapy

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