Electroactive 3D Scaffolds Based on Silk Fibroin and Water‐Borne Polyaniline for Skeletal Muscle Tissue Engineering

Zhang, Mengyao; Guo, Baolin

doi:10.1002/mabi.201700147

Cited by 56 publications

(25 citation statements)

References 58 publications

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“…The results showed that dually crosslinked GelMA-alginate bioinks with 8% (w/v) alginate promoted the myotube formation compared to other bioinks. This result is consistent with the previous report that scaffolds for myoblast culture require a compression modulus of at least 200 kPa to tolerate cell-induced deformation in the differentiation process [24,25]. High mechanical strength of dually crosslinked GelMA-8% (w/v) alginate bioinks supported cell-cell contact and thereby cell-cell fusion during the differentiation process more as compared to other bioinks.…”

Section: Resultssupporting

confidence: 91%

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

et al. 2019

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Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL were used as the cell model. The effects of alginate concentration (0, 6, and 8% (w/v)) and crosslinking mechanism (UV crosslinking or ionic crosslinking with UV crosslinking) on printability, cell viability, proliferation, and differentiation of bioinks were studied. The results showed that 10% (w/v) GelMA-8% (w/v) alginate crosslinked using UV light and 0.1 M CaCl2 provided the optimum niche to induce muscle tissue formation compared to other hydrogel compositions. Furthermore, metabolic activity of cells in GelMA bioinks was improved by addition of oxygen-generating particles to the bioinks. It is hoped that such bioprinted muscle tissues may find wide applications in drug screening and tissue regeneration.

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Section: Resultssupporting

confidence: 91%

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

et al. 2019

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“…The protein‐based scaffolds hold promise in tissue engineering, since the extracelluar matrix is composed of the natural proteins and glycosaminoglycans . Therefore, many natural proteins have been applied to fabricate tissue engineering scaffold, such as collagen and silk fibroin (SF) . SF, which is synthesized by an enormous variety of insect and spider species, has received attentions as potential biomedical purposes in the field of tissue engineering and drug delivery system since of its impressive mechanical strength, low inflammation reaction, and degradation properties .…”

Section: Introductionmentioning

confidence: 99%

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Zhang

Liu

et al. 2018

J Biomedical Materials Res

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In tissue regenerative medicine, developing tunable degradation rate of biomaterials for predictive functional outcomes remains critical. The implanted scaffolds should degrade gradually along with the tissue regeneration, and the optimal degradation rate of scaffold depends on the tissue type to be regenerated. Herein, the tunable degradation rates of silk fibroin (SF) scaffolds were fabricated through controlling dissolution, hydrolyzing conditions, and freeze-drying. The pore size, water adsorption capacity, and mechanical properties of scaffolds were associated with their average molecular weights. Moreover, in vitro cytotoxicity tests demonstrated that rapid degradation of SF scaffolds would facilitate the Schwann cells proliferation. Furthermore, in vitro enzymatic degradation and in vivo subcutaneous implantation experiments illustrated that SF scaffolds degradation behaviors could be well regulated. Immunohistochemistry staining experiments suggested that SF scaffold-degradation products could promote the endothelial cells proliferation. These results indicate that SF tunable degradation rates are promising candidates in regenerative medicine.

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“… Electroconductive Du et al [ 43 ] Poly (citric acid-octanediol-polyethylene glycol)(PCE)-graphene (PCEG) nanocomposite Murine C2C12 myoblasts In vivo Addition of reduced graphene oxide (RGO) improved scaffold mechanical properties and electrical conductivity. Addition of RGO increased scaffold myofiber and capillary density in vivo Zhang et al [ 84 ] SF/PASA: Silk fibroin with poly(aniline‐co‐N‐(4‐sulfophenyl) aniline) Murine L929 fibroblast and C2C12 myoblasts In vitro Characterisation of scaffold electroconductivity and biodegradability. Increasing PASA content enhanced myogenic differentiation of C2C12 myoblasts Ostrovidov et al [ 69 ] Gelatin-polyaniline (PANI) electrospun nanofibers Murine C2C12 myoblastis In vitro The addition of PANI increased nanofiber electroconductivity by 10 4 S/cm.…”

Section: Scaffold Use For Skeletal Muscle Engineeringmentioning

confidence: 99%

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Langridge

Butler

2021

J Mater Sci: Mater Med

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Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by limited donor tissue and significant donor morbidity. Tissue-engineered skeletal muscle constructs promise the potential to revolutionise the treatment of VML through the regeneration of functional skeletal muscle. Herein, we review the current status of tissue engineering approaches to VML; firstly the design of biocompatible tissue scaffolds, including recent developments with electroconductive materials. Secondly, we review the progenitor cell populations used to seed scaffolds and their relative merits. Thirdly we review in vitro methods of scaffold functional maturation including the use of three-dimensional bioprinting and bioreactors. Finally, we discuss the technical, regulatory and ethical barriers to clinical translation of this technology. Despite significant advances in areas, such as electroactive scaffolds and three-dimensional bioprinting, along with several promising in vivo studies, there remain multiple technical hurdles before translation into clinically impactful therapies can be achieved. Novel strategies for graft vascularisation, and in vitro functional maturation will be of particular importance in order to develop tissue-engineered constructs capable of significant clinical impact.

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Electroactive 3D Scaffolds Based on Silk Fibroin and Water‐Borne Polyaniline for Skeletal Muscle Tissue Engineering

Cited by 56 publications

References 58 publications

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

Tailoring degradation rates of silk fibroin scaffolds for tissue engineering

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

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