Electroacupuncture ameliorates neuronal injury by Pink1/Parkin-mediated mitophagy clearance in cerebral ischemia-reperfusion

Wang, Huanyuan; Chen, Su-Hui; Zhang, Yamin; Xu, Hong; Sun, Hua

doi:10.1016/j.niox.2019.07.004

Cited by 107 publications

(71 citation statements)

References 27 publications

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“…Inducible NOS (iNOS) expression can be upregulated by ischemia and delayed reperfusion after a stroke episode, which plays a pathological role in oxidative/nitrative stress [ 76 ]. In I/R injury rats, EA or MA effectively alleviated oxidative/nitrative stress-induced mitochondrial dysfunction and provided neuroprotection against infarct expansion by downregulating iNOS expression and peroxynitrite production, which ameliorated cognitive impairment and improved neurological function [ 77 – 79 ].…”

Section: Acupuncture Regulates Oxidative Stress In Ischemic Strokementioning

confidence: 99%

“…The Pink1/Parkin pathway is an important pathway in regulating mitophagy clearance, which can maintain neuronal mitochondrial quality [ 86 ]. By activating Pink1/Parkin-mediated mitophagy clearance, EA could decrease dysfunctional mitochondria and ROS production in transient MCAO models [ 77 ]. Moreover, EA also downregulated the level of iNOS via regulating the p38 mitogen-activated protein kinase (MAPK)/nuclear factor- κ B (NF- κ B) signaling pathway, which contributed salutary effects to the attenuation of nitrative stress as well as inflammation after cerebral I/R [ 79 ].…”

Section: Acupuncture Regulates Oxidative Stress In Ischemic Strokementioning

confidence: 99%

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Mechanisms of Acupuncture in the Regulation of Oxidative Stress in Treating Ischemic Stroke

Wang

et al. 2020

Oxidative Medicine and Cellular Longevity

122

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Ischemic stroke is the major type of cerebrovascular disease usually resulting in death or disability among the aging population globally. Oxidative stress has been closely linked with ischemic stroke. Disequilibrium between excessive production of reactive oxygen species (ROS) and inherent antioxidant capacity leads to subsequent oxidative damage in the pathological progression of ischemic brain injury. Acupuncture has been applied widely in treating cerebrovascular diseases from time immemorial in China. This review mainly lays stress on the evidence to illuminate the possible mechanisms of acupuncture therapy in treating ischemic stroke through regulating oxidative stress. We found that by regulating a battery of molecular signaling pathways involved in redox modulation, acupuncture not only activates the inherent antioxidant enzyme system but also inhibits the excessive generation of ROS. Acupuncture therapy possesses the potential in alleviating oxidative stress caused by cerebral ischemia, which may be linked with the neuroprotective effect of acupuncture.

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Section: Acupuncture Regulates Oxidative Stress In Ischemic Strokementioning

confidence: 99%

Section: Acupuncture Regulates Oxidative Stress In Ischemic Strokementioning

confidence: 99%

Mechanisms of Acupuncture in the Regulation of Oxidative Stress in Treating Ischemic Stroke

Wang

et al. 2020

Oxidative Medicine and Cellular Longevity

122

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“…Mitophagy, as a way to clear damaged mitochondria, plays a vital role in CI/R [6], but it still remains controversial. Recent study has reported that electroacupuncture improves CI/R injury by activating Pink1/Parkin-induced mitophagy [7]. Pink1/Parkin-mediated mitophagy ameliorates CI/R injury by eliminating ATF4 and NLRP3 inflammasome [8].…”

Section: Introductionmentioning

confidence: 99%

LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells

Deng

Ren

et al. 2020

Biol Res

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Background Long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 (SNHG14) is associated with cerebral ischemia–reperfusion (CI/R) injury. This work aims to explore the role of SNHG14 in CI/R injury. Methods HT22 (mouse hippocampal neuronal cells) cell model was established by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. The interaction among SNHG14, miR-182-5p and BNIP3 was verified by luciferase reporter assay. Flow cytometry, western blot and quantitative real-time PCR were performed to examine apoptosis, the expression of genes and proteins. Results SNHG14 and BNIP3 were highly expressed, and miR-182-5p was down-regulated in the OGD/R-induced HT22 cells. OGD/R-induced HT22 cells exhibited an increase in apoptosis. SNHG14 overexpression promoted apoptosis and the expression of cleaved-caspase-3 and cleaved-caspase-9 in the OGD/R-induced HT22 cells. Moreover, SNHG14 up-regulation enhanced the expression of BNIP3, Beclin-1, and LC3II/LC3I in the OGD/R-induced HT22 cells. Furthermore, SNHG14 regulated BNIP3 expression by sponging miR-182-5p. MiR-182-5p overexpression or BNIP3 knockdown repressed apoptosis in OGD/R-induced HT22 cells, which was abolished by SNHG14 up-regulation. Conclusion Our study demonstrates that lncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells. Thus, SNHG14/miR-182-5p/BINP3 axis may be a valuable target for CI/R injury therapies.

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“…Mitochondrial autophagy is a selective form of autophagy that removes unwanted or damaged mitochondria (37,38). PinK1/Parkin-mediated induction of mitophagy and its protective effects have been demonstrated in cerebral ischemia (39)(40)(41). However, little is known regarding the role of mitophagy in resveratrol-induced neuroprotection.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol alleviates oxygen/glucose deprivation/reoxygenation‑induced neuronal damage through induction of mitophagy

2020

Mol Med Rep

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resveratrol confers neuroprotective effects in cerebral ischemia; however, the involvement of mitophagy in the neuroprotective function of resveratrol remains unclear. The aim of the present study was to investigate whether resveratrol exerts neuroprotective effects on primary cortical neurons subjected to oxygen/glucose deprivation/reoxygenation (oGd/r) via modulating mitophagy. The data demonstrated that resveratrol at 1-10 µM during reoxygenation improved cell viability and suppressed apoptosis following oGd/r in a concentration-dependent manner. Moreover, resveratrol alleviated oGd/r-induced loss of mitochondrial membrane potential and excessive oxidative stress. confocal imaging of lc3 and ToM20 antibody-labeled mitochondria, as well as western blot analysis, demonstrated that mitophagy was further enhanced following resveratrol treatment. in addition, resveratrol was revealed to stimulate the phosphatase and tensin homolog-induced kinase 1/Parkin pathway. Mitophagy inhibition then inhibited the protective effects of resveratrol. These results indicated that resveratrol exerts its protective effects against oGd/r damage, at least in part, by promoting mitophagy.

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Electroacupuncture ameliorates neuronal injury by Pink1/Parkin-mediated mitophagy clearance in cerebral ischemia-reperfusion

Cited by 107 publications

References 27 publications

Mechanisms of Acupuncture in the Regulation of Oxidative Stress in Treating Ischemic Stroke

Mechanisms of Acupuncture in the Regulation of Oxidative Stress in Treating Ischemic Stroke

LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells

Resveratrol alleviates oxygen/glucose deprivation/reoxygenation‑induced neuronal damage through induction of mitophagy

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