Electroacupuncture Attenuates Bone Cancer Pain and Inhibits Spinal Interleukin-1β Expression in a Rat Model

Zhang, Rui-Xin; Li, Aihui; Liu, Bing; Wang, Linbo; Ren, Ke; Qiao, Jian‐Tian; Berman, Brian M.; Lao, Lixing

doi:10.1213/01.ane.0000284705.34629.c5

Cited by 65 publications

(54 citation statements)

References 37 publications

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“…A previous study demonstrated that EA inhibits bone cancer-caused IL-1β expression and bone cancer pain [24]. IL-1β also promotes NR1 phosphorylation [6].…”

Section: Fig (5)mentioning

confidence: 95%

“…A symmetrical biphasic wave was delivered to each electrode so that it was alternately positive and negative to stimulate the bilateral needles. To minimize discomfort, stimulation intensity was gradually increased over a period of 2 min to 2 mA, which we have found to be the maximum level that can be tolerated by unrestrained rats [24]. During EA treatment, each rat was placed under an inverted clear plastic chamber approximately 5" × 8" × 11" and neither restrained nor given any anesthetic.…”

Section: Electroacupuncturementioning

confidence: 99%

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Electroacupuncture Inhibits Spinal Interleukin-17A to Alleviate Inflammatory Pain in a Rat Model

Meng¹,

Lao²,

Shen³

et al. 2013

TOPAINJ

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Although acupuncture analgesia has been reported in clinical trials, its mechanisms have been unclear. It was recently reported that spinal astrocytes-produced interleukin-17A (IL-17A) facilitates inflammatory pain. Hypothesizing that electroacupuncture (EA) would suppress inflammation-enhanced IL-17A synthesis to inhibit pain, we induced hyperalgesia, as measured by decreased paw withdrawal latency (PWL) to a noxious thermal stimulus, by subcutaneously injecting complete Freund's adjuvant (CFA, 0.08 ml, 40 µg Mycobacterium tuberculosis) into the hind paws of rats, or intrathecal (i.t.) IL-17A (400 ng in 10 µl) into the lumbar spinal cord. We then gave EA at acupoint GB30 for two 20-min periods, once immediately after CFA or IL-17A administration and again 2 h post-injection. For sham control, EA needles were inserted into GB30 without stimulation. PWL was measured before and 2.5 and 24 h after injection. Spinal IL-17A, IL-17 receptor A (IL-17RA), and phosphorylated NR1, an essential subunit of the N-methyl D-aspartate receptor (NMDAR), were determined 24 h post-CFA or -IL-17A using immunohistochemistry and western blot. Compared to sham control, EA inhibited CFA-caused thermal hyperalgesia 2.5 and 24 h post-CFA and concurrently suppressed inflammation-enhanced IL-17A and IL-17RA synthesis and NR1 phosphorylation in the ipsilateral spinal cord. EA inhibited IL-17A-produced thermal hyperalgesia, IL-17RA synthesis and NR1 phosphorylation. Our data suggest that EA inhibits inflammatory pain by blocking spinal IL-17A synthesis. Since previous study shows that IL-17A is located in astrocytes and IL-17RA and NR1 are in neurons, the data suggest that EA alleviates pain by modulating glia-neuronal interactions that involve IL-17A, IL-17RA, and NR1 phosphorylation.

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“…A previous study demonstrated that EA inhibits bone cancer-caused IL-1β expression and bone cancer pain [24]. IL-1β also promotes NR1 phosphorylation [6].…”

Section: Fig (5)mentioning

confidence: 95%

Section: Electroacupuncturementioning

confidence: 99%

Electroacupuncture Inhibits Spinal Interleukin-17A to Alleviate Inflammatory Pain in a Rat Model

Meng¹,

Lao²,

Shen³

et al. 2013

TOPAINJ

Self Cite

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“…[22][23][24][25][26] A recent study also showed that electroacupuncture alleviates bone cancer pain by suppressing spinal expression of interleukin-1b in glial cells that are involved in the spinal transmission and processing of noxious inputs from the peripheral sites and facilitates pain. 27 It is conceivable that acupuncture reduces BIPN symptoms by suppressing activities of glial cells and stimulating neurohormonal pathways, which increases endorphine release and reduces proinflammatory cytokines. 28 Lastly, animal studies suggest that acupuncture might stimulate nerve growth factors and accelerate nerve regeneration.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Painful Bortezomib-Induced Peripheral Neuropathy Following Acupuncture Treatment in a Case Series of Multiple Myeloma Patients

Bao

Lao

Medeiros

et al. 2012

Medical Acupuncture

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Background: Bortezomib-induced peripheral neuropathy (BIPN) is a common and severe dose-limiting side effect in multiple myeloma (MM) patients. Treatment with narcotics, antidepressants, and anticonvulsants are of limited value in reducing symptoms, and they have the potential for significant side effects. Acupuncture has been reported to be effective in treating neuropathic pain. There has been limited reporting on the effect of acupuncture in treating BIPN. Objective: The aim of this study was to report on the effect of acupuncture in treating BIPN. Design and Patients: This study was a retrospective case series of five MM patients experiencing painful BIPN. Intervention: The patients were treated with the same acupuncture protocol on a weekly basis, adjusted to twice a week or once every 2 weeks based on response. Treatment included insertion of disposable sterile acupuncture needles, which were retained for 25 minutes, at the following bilateral ear points: shen men, point zero, and two auricular points where electro-dermal signal was detected; and bilateral body acupuncture points: LI 4, TE 5, LI 11, ST 40, and Ba Feng in the upper and lower extremities. Main Outcome Measures: All patients were assessed for severity of painful BIPN using a 0-10 numeric pain scale, with one patient assessed using the Clinical Total Neuropathy Score, Functional Assessment of Cancer Therapy-Neurotoxicity questionnaire, and Neuropathy Pain Scale. Results: All five patients experienced immediate pain reduction after one acupuncture treatment. Two of three patients who had more than three acupuncture treatments experienced long-lasting pain reduction and function improvement. There were no adverse events associated with acupuncture. Conclusion: Acupuncture is a viable treatment option for MM patients experiencing painful BIPN. However, further prospective research is needed.

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“…At this point in time, there is not convincing evidence that acupuncture signiicantly reduces cancer pain in the human literature. That being said there are several studies using animal models of cancer bone pain that suggest that acupuncture can reduce bone tumor-induced pain including osteosarcoma-induced pain [1,106,107]. The animal data suggest that electroacupuncture can alleviate bone cancer pain, at least in part by suppressing IL-1β expression and by altering nerve innervation and the vasculature of osteosarcoma.…”

Section: Complementary/alternative Therapiesmentioning

confidence: 99%

Osteosarcomagenesis: Biology, Development, Metastasis, and Mechanisms of Pain

Smeester¹,

Moriarity²,

Beitz³

2017

Osteosarcoma - Biology, Behavior and Mechanisms

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Osteosarcoma is the most common primary cancer of the bone and third most common cancer in children and adolescents with approximately 900 new cases annually in the United States. A major facet of osteosarcoma is its high level of genomic instability, in particular chromosomal instability, which is the result of increased or decreased chromosome number in a cell. Furthermore, pain is the most common symptomatic feature of osteosarcoma that lacks efective therapy. Pain in osteosarcoma is relatively more complicated than many other painful conditions requiring a more thorough understanding of its etiology, pathobiology, and neurobiology to allow the development of beter therapies for reducing pain in osteosarcoma patients. Studies are underway to deine the diverse modalities of presentation, growth, development, metastases, and nociception in osteosarcoma. New data from human studies in combination with data from studies incorporating transgenic mouse models of osteosarcoma are providing valuable insights into the mechanisms underlying the development of both the tumor and the tumor-induced pain. These new data will undoubtedly lead to improved prognoses, as well as the development of novel therapeutics that will signiicantly decrease bone cancer pain.

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Electroacupuncture Attenuates Bone Cancer Pain and Inhibits Spinal Interleukin-1β Expression in a Rat Model

Abstract: The data suggest that EA alleviates bone cancer pain, at least in part by suppressing IL-1beta expression. The results support the clinical use of EA in the treatment of cancer pain.

Cited by 65 publications

References 37 publications

Electroacupuncture Inhibits Spinal Interleukin-17A to Alleviate Inflammatory Pain in a Rat Model

Electroacupuncture Inhibits Spinal Interleukin-17A to Alleviate Inflammatory Pain in a Rat Model

Improvement of Painful Bortezomib-Induced Peripheral Neuropathy Following Acupuncture Treatment in a Case Series of Multiple Myeloma Patients

Osteosarcomagenesis: Biology, Development, Metastasis, and Mechanisms of Pain

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