Electroacupuncture Pretreatment Elicits Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Rats Associated with Transient Receptor Potential Vanilloid 1-Mediated Anti-Oxidant Stress and Anti-Inflammation

Long, Min; Wang, Zhigang; Zheng, Dan; Chen, Jiaojiao; Tao, Wenting; Wang, Lei; Yin, Ning; Chen, Zebin

doi:10.1007/s10753-019-01040-y

Cited by 43 publications

(32 citation statements)

References 29 publications

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“…miR-340-5p has also been demonstrated to suppress hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes (16). oxidative stress and inflammation are the main causes of cerebral ischemia-reperfusion injury, which has been established through numerous previous studies (17)(18)(19)(20)(21). For example, in one previous study, mir-340-5p was found to inhibit inflammation and oxidative stress in cardiomyocytes and serve a role in cerebral ischemia-reperfusion injury (16).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4

Wang

Liu

2020

Mol Med Rep

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The expression levels of microrna (mir)-340-5p are reportedly decreased in the peripheral blood during acute ischemic stroke; however, the direct effect and mechanism of action of mir-340-5p in ischemic stroke remains largely unknown. The present study aimed to investigate the effects of mir-340-5p, and its mechanism of action, on Pc12 cells following oxygen-glucose deprivation/reperfusion (oGd/r) induction. oGd/r-induced Pc12 cells served as the cellular model and subsequently, mrna expression levels of mir-340-5p and neuronal differentiation 4 (neurod4) were analyzed using reverse transcription-quantitative Pcr. Tumor necrosis factor-α, interleukin (il)-1β and il-6 expression levels were detected using ELISA kits, and flow cytometry was used to determine the rate of cellular apoptosis. in addition, a nitric oxide (no) synthase activity assay kit was used to detect no levels and a nadPH assay kit was used to measure nadPH levels. Western blotting was also performed to analyze protein expression levels of bax, bcl-2, cleaved caspase 3 and phosphorylated endothelial noS (enoS), and the target gene of mir-340-5p was predicted using TargetScan software and verified using a dual-luciferase reporter assay. The expression levels of mir-340-5p were decreased in Pc12 cells following oGd/r induction and Neurod4 was identified as a target gene of miR-340-5p. In addition, miR-340-5p overexpression reduced inflammation, apoptotic rate, no production and nadPH levels, in addition to increasing enoS expression in Pc12 cells following oGd/r induction. notably, the overexpression of neurod4 reversed the aforementioned effects of mir-340-5p on Pc12 cells following OGD/R induction. In conclusion, the findings of the present study suggested that mir-340-5p may protect Pc12 cells against oGd/r through targeting neurod4, which could provide important implications for the treatment of ischemia-reperfusion injury based on mir-340-5p expression levels in vivo.

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Section: Introductionmentioning

confidence: 99%

Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4

Wang

Liu

2020

Mol Med Rep

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“…TRPV1 was also found to be involved in ischemic stroke and neurological deficits in a murine model of middle cerebral artery occlusion [52]. Pretreatment with electroacupuncture had a neuroprotective effect in rats with cerebral ischemia-reperfusion injury through reducing the infarct volumes, decreasing the risk of oxidative stress injury, inhibiting inflammatory cytokine production, and suppressing the expression of TRPV1 [53].…”

Section: Plos Onementioning

confidence: 97%

Acupuncture decreased the risk of stroke among patients with fibromyalgia in Taiwan: A nationwide matched cohort study

et al. 2020

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Background The aim of this study was to investigate the effectiveness of acupuncture in decreasing the risk of stroke in patients with fibromyalgia. Methods We enrolled patients who was newly diagnosed as having fibromyalgia between 1 January, 2000 and 31 December, 2010 from the Taiwanese National Health Insurance Research Database. The claim data for both the acupuncture cohort and non-acupuncture cohort were assessed from the index date to 31 December, 2013. A Cox regression model adjusted for age, sex, comorbidities, and drugs use was used to compare the hazard ratios of the two cohorts. The cumulative incidence of stroke was estimated by using the Kaplan-Meier method. Results After performing a propensity score matching with a 1:1 ratio, there were 65,487 patients in the acupuncture and non-acupuncture cohorts with similar distributions in the baseline characteristics. The cumulative incidence of stroke was significantly lower in the acupuncture cohort (log-rank test, p < 0.001). In the follow-up period, 4,216 patients in the acupuncture cohort (11.01 per 1000 person-years) and 6,849 patients in the non-acupuncture cohort (19.82 per 1000 person-years) suffered from stroke (adjusted HR 0.53, 95% CI 0.51-0.55).

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“…Another study also reported that EA at GV20 and GV24 increased the Bcl-2/Bax ratio [50]. In addition, EA pretreatment at not only prevents P38/MAPK activation, but also increased the activities of GSH and SOD and downregulated the levels of MDA [51].…”

Section: Inhibiting Downstream Of the Ros Pathway To Cell Apoptosis Omentioning

confidence: 83%

Effect of Acupuncture on Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury

Chen

Hsieh

2020

Antioxidants

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In this article, we review how acupuncture regulates oxidative stress to prevent ischemia–reperfusion injury. We electronically searched databases, including PubMed, Clinical Key and the Cochrane Library, from their inception to November 2019 by using the following medical subject headings and keywords: acupuncture, ischemia-reperfusion injury, oxidative stress, reactive oxygen species, and antioxidants. We concluded that acupuncture is effective in treating oxidation after ischemia-reperfusion injury. In addition to increasing the activity of antioxidant enzymes and downregulating the generation of reactive oxygen species (ROS), acupuncture also repairs the DNA, lipids, and proteins attacked by ROS and mediates downstream of the ROS pathway to apoptosis.

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Electroacupuncture Pretreatment Elicits Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Rats Associated with Transient Receptor Potential Vanilloid 1-Mediated Anti-Oxidant Stress and Anti-Inflammation

Cited by 43 publications

References 29 publications

Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4

Protective effect of microRNA‑340‑5p against oxygen‑glucose deprivation/reperfusion in PC12 cells through targeting neuronal differentiation 4

Acupuncture decreased the risk of stroke among patients with fibromyalgia in Taiwan: A nationwide matched cohort study

Effect of Acupuncture on Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury

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