Electroacupuncture Prevents Ovariectomy-Induced Osteoporosis in Rats: A Randomised Controlled Trial

Zeng

et al. 2017

Bioelectromagnetics

Self Cite

The aim of this study is to explore the effect of timing of initiation of pulsed electromagnetic field (PEMF) therapy on bone mass, microarchitecture, and biomechanical properties, and to investigate receptor activator of NF-kB (RANK) expression in ovariectomized (OVX) rats. Sixty female Sprague-Dawley rats were randomly divided into two equal batches of three groups each (10 rats in each group). The first batch comprised of sham-operated (Sham-0 group), ovariectomized (OVX-0 group), and ovariectomized plus treated with PEMF starting from the day of OVX (Early PEMF group). The second batch comprised of sham-operated (Sham-12 group), ovariectomized (OVX-12 group), and ovariectomized plus treated with PEMF starting 12 weeks after OVX (Late PEMF group). Rats (whole body) in the early and late PEMF groups were exposed to PEMF (3.8 mT peak, 8 Hz pulse burst repetition rate). After 12 weeks of PEMF therapy, Early PEMF prevented OVX-induced deterioration in bone mineral density (BMD) and mechanical properties in lumbar vertebral body and femur, and deterioration in bone microarchitecture in lumbar vertebral body and proximal tibia. Late PEMF intervention only inhibited deterioration of BMD, bone microarchitecture, and mechanical properties in lumbar vertebral body. Both early and late PEMF therapy suppressed RANK protein expression in OVX rats without a concomitant effect on RANK mRNA expression. These results demonstrate that timing of initiation of PEMF therapy plays an important role in achieving optimal beneficial effects. The specific PEMF parameters may exert these favorable biological responses, at least partially, via inhibition of protein expression of RANK. Bioelectromagnetics. 38:456-465, 2017. © 2017 Wiley Periodicals, Inc.

Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Effect of intervention initiation timing of pulsed electromagnetic field on ovariectomy‐induced osteoporosis in rats

Zeng

et al. 2017

Bioelectromagnetics

Self Cite

“…First, the mechanisms underlying the synergistic efficacy of IBN + PEMF are not fully understood, and further research is needed. Secondly, although osteoporosis can be successfully established using 3‐month‐old rats (matured but young) [Sehmisch et al, ; Yang et al, ; Zhou et al, ,, , ], growth‐dependent effects may have influenced the results.…”

Section: Discussionmentioning

confidence: 99%

“…Animals were housed in stainless‐steel wire cages under standard laboratory conditions (12 h light/dark cycle, relative humidity of 55 ± 5%, 24 ± 2 °C) with free access to water and food. After 1 week of acclimatization, all rats were subjected to either bilateral ovariectomy or sham surgery, as we previously described [Zhou et al, ,, , ].…”

Section: Methodsmentioning

confidence: 99%

Effects of combined treatment with ibandronate and pulsed electromagnetic field on ovariectomy‐induced osteoporosis in rats

Xie

et al. 2016

Bioelectromagnetics

Self Cite

Ibandronate (IBN) and pulsed electromagnetic field (PEMF) have each shown positive effects for treating osteoporosis, but no study has evaluated the relative effects of these treatments combined. This study investigated the effects of IBN þ PEMF on bone turnover, mineral density, microarchitecture, and biomechanical properties in an ovariectomized (OVX) rat model of osteoporosis. Fifty 3-month-old rats were randomly apportioned to receive a sham-operation (n ¼ 10), or ovariectomy (n ¼ 40). The latter group was equally divided as the model (OVX control) or to receive IBN, PEMF, or IBN þ PEMF. Beginning the day after surgery, the IBN and IBN þ PEMF groups received weekly subcutaneous IBN; the PEMF and IBN þ PEMF groups were given daily PEMF during the same 12 weeks. After 12 weeks of treatments, biochemical parameters, bone mineral density (BMD), microarchitecture parameters, biomechanical properties, and some metabolic modulators that are involved in bone resorption were compared. The L5 lumbar vertebral body BMDs of the IBN, PEMF, and IBN þ PEMF groups were 121.6%, 119.5%, and 139.6%; maximum loads were 111.4%, 112.7%, and 121.9%; and energy to failure was 130.8%, 129.2%, and 154.9% of the OVX model, respectively. The IBN þ PEMF group had significantly lower levels of serum tartrate-resistant acid phosphatase 5b, and greater improvement in BMD, bone microarchitecture, and strength of the lumbar spine compared with monotherapy groups. Results showed that IBN þ PEMF had a more favorable effect on the lumbar spine in this osteoporosis model than did either monotherapy. Bioelectromagnetics. 38:31-40, 2017.

“…To evaluate the bone strength, the three-point bending examination for the left femur was performed by the AG-IS (Shimadzu, Japan) biomechanical testing system according to our previous description [24]. The femurs were slowly thawed and warmed to room temperature.…”

Section: Biomechanical Examinationmentioning

confidence: 99%

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

et al. 2014

Endocrine

Self Cite

Evidences have shown that pulsed electromagnetic fields (PEMFs) can partially prevent bone loss in streptozotocin (STZ)-induced diabetic rats. However, the precise mechanisms accounting for these favorable effects are unclear. This study aimed to investigate the effects of PEMFs on bone mass and receptor activator of nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) and Wnt/β-catenin signaling pathway in STZ rats. Thirty 3-month-old Sprague Dawley rats were randomly divided into the following three groups (n = 10): control group (injection of saline vehicle), DM group (injection of STZ), and PEMFs group (injection of STZ + PEMFs exposure). One week following injection of STZ, rats in the PEMFs group were subject to PEMFs stimulus for 40 min/day, 5 days/week, and lasted for 12 weeks. After 12 week intervention, the results showed that PEMFs increased serum bone-specific alkaline phosphatase level and bone mineral density, and inhibited deterioration of bone microarchitecture and strength in STZ rats. Furthermore, PEMFs up-regulated the mRNA expressions of low-density lipoprotein receptor-related protein 5, β-catenin and runt-related gene 2 (Runx2), and down-regulated dickkopf1 in STZ rats. However, mRNA expressions of RANKL and OPG were not affected by PEMFs. PEMFs can prevent the diabetes-induced bone loss and reverse the deterioration of bone microarchitecture and strength by restoring Runx2 expression through regulation of Wnt/β-catenin signaling, regardless of its no glucose lowering effect.