Electroacupuncture Reverses CUMS-Induced Depression-Like Behaviors and LTP Impairment in Hippocampus by Downregulating NR2B and CaMK II Expression

Abstract

Objective. Depression is a global mental health problem with high disability rate, which brings a huge disease burden to the world. Electroacupuncture (EA) has been shown to be an effective method for the treatment of depression. However, the mechanism underling the antidepressant effect of EA has not been clearly clarified. The change of synaptic plasticity is the focus in the study of antidepressant mechanism. This study will observe the effect of EA on LTP of hippocampal synaptic plasticity and explore its possible mechanism. Methods. The depression-like behavior rat model was established by chronic unpredictable mild stress (CUMS). EA stimulation (Hegu and Taichong) was used to treat the depressed rats. The depression-like behavior of rats was tested by weight measurement, open field test, depression preference test, and novelty suppressed feeding test. Long-term potentiation (LTP) was recorded at CA1 synapses in hippocampal slices by electrophysiological method. N-methyl-D-aspartate receptor subunit 2B (NR2B) and calmodulin-dependent protein kinase II (CaMK II) protein levels were examined by using western blot. Results. After the establishment of CUMS-induced depression model, the weight gain rate, sucrose preference rate, line crossing number, and rearing times of rats decreased, and feeding time increased. At the same time, the LTP in hippocampus was impaired, and the expressions of NR2B and CaMK II were upregulated. After EA treatment, the depression-like behavior of rats was improved, the impairment of LTP was reversed, and the expression levels of NR2B and CaMK II protein were downregulated. Conclusion. EA can ameliorate depression-like behaviors by restoring LTP induction, downregulating NR2B and CaMK II expression in CUMS model rats, which might be part of the mechanism of EA antidepressant.

Electroacupuncture prevents astrocyte atrophy to alleviate depression

“…Pharmacological management of MDD remains a challenge, with many patients being resistant to traditional antidepressants. Electroacupuncture (EA) stimulation is employed as an effective and safe therapy to treat multiple diseases including inflammation [ 22 , 23 ], Parkinson’s disease [ 24 ], insomnia [ 25 – 27 ], anxiety [ 28 ] and depression [ 29 – 31 ]. The EA at selective acupoints Dazhui (DU14) and Baihui (DU20) was shown to restore inhibitory/excitatory balance and improve cognition in mouse models of Alzheimer’s disease [ 32 ].…”

Electroacupuncture prevents astrocyte atrophy to alleviate depression

“…Pharmacological management of MDD remains a challenge, with many patients being resistant to traditional antidepressants. Electroacupuncture (EA) stimulation is employed as an effective and safe therapy to treat multiple diseases including inflammation (Liu et al, 2021; Liu et al, 2020), Parkinson’s disease (Tamtaji et al, 2019), insomnia (Ruan et al, 2009; Yin et al, 2017; Yin et al, 2020), anxiety (Shen et al, 2020) and depression (Jiang et al, 2021; Xu et al, 2020b; Yao et al, 2021). The EA at selective acupoints Dazhui (DU14) and Baihui (DU20) was shown to restore inhibitory/excitatory balance and improve cognition in mouse models of Alzheimer’s disease (Li et al, 2023).…”