Electroanalytical determination of d(GCGAAGC) hairpin

Trnková, Libuše; Postbieglová, Irena; Holík, Miroslav

doi:10.1016/j.bioelechem.2003.09.012

Cited by 28 publications

(34 citation statements)

References 18 publications

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“…3a). At reference scan rate 200 mV/s the EVLS detection limit of H3 was determined with value 10 ng/mL (i.e., 3.8 nM) and confirmed chemometrically using the confidence ellipse [21]. From Figure 3b is obvious that the dependence of I p on t a has the form of adsorption isotherm not starting from zero.…”

Section: Resultsmentioning

confidence: 99%

“…Note that Equation 2 has been obtained under the assumption that the sweep has started at sufficiently positive potentials where the electrode reaction did not proceed. Thus, in the case of the total adsorbed electroactive substance, the elimination gives a much sharper peak (p) which passes through zero current line into a sharp opposite peak (cp), and creates the characteristic peak -counterpeak form [20,21,32,33,36,40]. The Equation 2 can be written as:…”

Section: Theorymentioning

confidence: 99%

“…In case the electrode process controlled only by diffusion, the transformation of the I -E curve of the electroactive species is given by Equation 1, but the adsorption preceding an electrode process can change this elimination curve. For irreversible current transformation of an adsorbed electroactive substance, the current -potential curve [20,21,32,33,36,39,40] has been used:…”

Section: Theorymentioning

confidence: 99%

“…Recently, we have reported about the resolution of A and C reduction signals in mixtures of short homo-oligonucleotides (dA 9 and dC 9 ) [20] and in d(GCGAAGC) hairpin by means of elimination voltammetry with linear scan (EVLS) [21].…”

Section: Introductionmentioning

confidence: 99%

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Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero‐Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides

Trnková¹,

Jelen²,

Postbieglová³

2006

Electroanalysis

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Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents (I c , I k ) and conserves the diffusion current (I d ). Since for an adsorbed electroactive substance this elimination function yields a well readable peak -counterpeak signal, the adsorptive stripping (AdS) procedure was favorably used. The adsorption of ODNs was carried out at À 0.1 V for accumulation time of 120 s under stirring. It was found that heights and potentials of LSV signals were affected by ODNs concentrations, pH, scan rates, time of accumulation, and stirring speed during the adsorption. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Our results showed that the EVLS in connection with the AdS procedure is a useful tool for qualitative and quantitative studies of short ODNs and a promising sensitive method for the development of electrochemical sensor following the ODN sequences.

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Section: Resultsmentioning

confidence: 99%

Section: Theorymentioning

confidence: 99%

Section: Theorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero‐Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides

Trnková¹,

Jelen²,

Postbieglová³

2006

Electroanalysis

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“…The elimination voltammetry with linear scan (EVLS) has been successfully applied to data analysis of oligodeoxynucleotides (ODNs) signal, aiming at better understanding of basic electrochemical processes on working electrode surfaces, and for better resolution of voltammetric signals of A and C in ODNs [14,16,32]. Using hanging mercury drop electrode (HMDE), A and C produce closely lying, overlapping reduction cathodic signals.…”

Section: Introductionmentioning

confidence: 99%

Resolution of Overlapped Reduction Signals in Short Hetero‐oligonucleotides by Elimination Voltammetry

Mikelová¹,

Trnková²,

Jelen³

et al. 2007

Electroanalysis

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The resolution of overlapped reduction voltammetric signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C, but with the same ratio (A/C ¼ 1/2), was carried out by elimination voltammetry with linear scan (EVLS). The EVLS, as a mathematical transformation of voltammetric total currents measured at different scan rates, is capable to yield very sensitive resolution of potentially closed signals, which cannot be separated by common electrochemical methods. The best resolution of A and C signals was observed when the EVLS function eliminating the charging and kinetic currents (I c , I k ) and conserving the diffusion current (I d ) was combined with adsorption stripping technique (AdS). The resulting peak-counterpeak signal made it possible not only to separate the responses of A and C residues, but also to follow the dynamics of adsorption processes on mercury electrode surfaces. Together with the effect of the accumulation time on peak-counterpeak signal, the effect of pH and temperature on this signal was studied. The relation between elimination signals and the structure changes of ODNs was discussed.

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Double Elimination Voltammetry of Short Oligonucleotides

2007

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In connection with adsorptive stripping (AdS) technique, elimination voltammetry with linear scan (EVLS) in double mode was successfully employed in the analysis of homo-ODNs: 5'-AAA AAA AAA-3' (dA 9 ) and 5'-CCC CCC CCC-3' (dC 9 ), and hetero-ODNs: 5'-CCC AAA CCC-3' (H3), 5'-CAC CAC CAC-3' (H4), and 5'-ACC CAC CCA-3' (H9) on hanging mercury drop electrode (HMDE). Analogously to single EVLS function E4 (conserving the diffusion current I d and eliminating kinetic and charging currents I k , I c ) for the electroactive substance adsorbed, the double EVLS function E4 yields well readable peak-counterpeaks of reducible nucleic acid bases (adenine A and cytosine C). In comparison with single EVLS these peak-counterpeaks are higher by more than one order of magnitude (twenty times for dA 9 , fourteen times for dC 9 , from eight to sixteen times for hetero-ODNs). The increase of reduction signals with higher resolution was also observed using other two EVLS functions in double mode, the functions E5 eliminating I c and I d , but conserving I k and E6 eliminating I d and I k , but conserving I c . The amplifications of double / single EVLS signals of A and C range from 3.4 to 8.4, and from 3.1 to 8.3 for E5 and E6, respectively. It was proved that AdS double EVLS offers a new, fast, simple and inexpensive electroanalytical tool, which can be considered as a device not only for very good resolution of A and C in ODNs, but also for sensitive detection of changes in the primary structure of nucleic acid bases in ODN chains, depending on experimental conditions, such as pH, temperature, and time and potential of accumulation.

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Electroanalytical determination of d(GCGAAGC) hairpin

Cited by 28 publications

References 18 publications

Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero‐Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides

Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides II. Hetero‐Oligodeoxynucleotides with Different Sequences of Adenine and Cytosine Nucleotides

Resolution of Overlapped Reduction Signals in Short Hetero‐oligonucleotides by Elimination Voltammetry

Double Elimination Voltammetry of Short Oligonucleotides

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