Electroantennogram Responses of <I>Nilaparvata lugens</I> (Homoptera: Delphacidae) to Plant Volatile Compounds

Abstract

Electroantennogram (EAG) recordings were made from the plaque organ receptors of the rice brown plant hopper, Nilaparvata lugens (Stål). The receptors of the distal plaque organ of both male and female hoppers responded to the air from above chopped rice plants, and those of females gave a larger response. Female hoppers, tested with 27 plant volatiles, gave dose-related responses to 16 of the compounds, including a range of green leaf volatiles. The aliphatic aldehydes hexanal and (E)-2-hexenal elicited a larger response than a range of green leaf alcohols. Of the remaining compounds, acetophenone, benzaldehyde, ethyl and methyl benzoate, and amyl and isoamyl acetate were among the most effective in eliciting responses. Receptor recovery times showed some variation between compounds. A comparison of the dose-related response of 16 compounds in paraffin oil and in pentane showed that considerably larger responses were recorded when the highly volatile solvent pentane was used.

Distinct Expression Profiles and Different Functions of Odorant Binding Proteins in Nilaparvata lugens Stål

“…6C, D, Table 3 and Table 4). Two green leaf volatiles (6-carbon compounds) Hexanal and (E)-2-Hexenal were among the most abundant volatiles of rice [39], and generate high Electroantennogram (EAG) response in BPH and some other Hemiptera insects [40], [41], [42]. As expected, NlugOBP3 could bind these two volatiles, although the Ki were not very high.…”

Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

“…The six ligands included three terpenes (β‐ionone, nerolidol, and safranal) and three benzoates (butyl benzoate, hexyl benzoate, and benzyl benzoate). SfurOBP11, NlugOBP8, and LstrOBP2 displayed moderate binding affinities of 12.43, 16.13, and 18.95 µM, respectively, with β‐ionone, which has been identified as a rice plant volatile and a constituent compound of rice plant extracts that is capable of evoking an electrophysiological response from BPH and attracting the three rice planthoppers . The ligand also showed relatively high binding abilities with other OBPs of WBPH and BPH .…”

Fine morphology of the antennae and mouthparts of Dentatissus damnosa (Chou & Lu) (Hemiptera: Issidae)

“…PO in D. damnosa may function as olfactory sensilla (Aljunid and Anderson, 1983;Bourgoin and Deiss, 1994;Youn, 2002). The olfactory function of plaque organ is reflected in the pores on the flat central area of the plate in D. damnosa.…”