Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell

Zani, Ana Clara Bonizol; Almeida, Erica Janaina Rodrigues de; Furlan, João Pedro Rueda; Pedrino, Matheus; Guazzaroni, María‐Eugenia; Stehling, Eliana Guedes; Andrade, Adalgisa R. de; Reginatto, Valéria

doi:10.1016/j.chemosphere.2023.139073

Cited by 13 publications

(9 citation statements)

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“…A considerable number of Pseudomonas species produce phenazine derivatives, such as PYO and phenazine-1-carboxamide (PCA) [ 57 ], which act as electron carriers [ 34 ]. In addition, pyoverdins, a class of yellowish-green siderophores, also exhibit redox properties and were reported as EET mediators in MFC [ 28 ]. Unfortunately, the redox signals observed in the voltammograms and the analysis of the BJa5 genome do not suggest that phenazine and pyoverdin are the redox-active species produced by BJa5.…”

Section: Resultsmentioning

confidence: 99%

“…Some bacteria, such as Pseudomonas aeruginosa, are considered weak electrogens, meaning that they are capable of EET at a low rate [ 27 ]. In this case, EET is mediated by phenazines and pyoverdines [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…are promising biocatalysts, being reported as one of the most representative genera in electrogenic biofilm consortia in MFCs in medium with acetate [ 35 , 36 ] or glycerol [ 37 ]. In addition to these natural characteristics, Pseudomonas can be easily modified with several synthetic engineering techniques and tools that can be useful to increase productivity in MFCs, such as increasing power density and current density [ 23 , 24 , 25 , 26 , 27 , 28 ]. Moreover, by synthetic biology techniques, EET kinetics can be improved, which is useful for microbial anodic electro-respiration and to promote pollutant removal at both the anode and cathode [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

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Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Microorganisms

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Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat various refuse, such as wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC’s anode. Moreover, Pseudomonas species can grow under low-oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species grown with 1 g.L−1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m−2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium’s potential as a synthetic biology non-model chassis. Through testing various genetic parts, including constitutive promoters, replication origins and cargos using pSEVA vectors as a scaffold, we assessed the bacterium’s suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Microorganisms

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“…Bacteria-producing electron transport mediators [164] can be used as an approach to reduce anodic overvoltage. Riboflavins [165], phenazines [166][167][168], pyocyanins [169], and quinones [170] are distinguished among endogenous media. It should be noted that the use of a mediator increases the current density by increasing the electron transfer rate [166,171].…”

Section: Integration Of Pmfc Into Hydrobotanical Sites For Wastewater...mentioning

confidence: 99%

State of the Art and Environmental Aspects of Plant Microbial Fuel Cells’ Application

Lepikash,

Lavrova,

Stom

et al. 2024

Energies

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Environmental pollution is becoming ubiquitous; it has a negative impact on ecosystem diversity and worsens the quality of human life. This review discusses the possibility of applying the plant microbial fuel cells (PMFCs) technology for concurrent processes of electricity generation and the purification of water and soil ecosystems from organic pollutants, particularly from synthetic surfactants and heavy metals. The review describes PMFCs’ functioning mechanisms and highlights the issues of PMFCs’ environmental application. Generally, this work summarizes different approaches to PMFC development and to the potential usage of such hybrid bioelectrochemical systems for environmental protection.

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“…Some bacteria, such as Pseudomonas aeruginosa, are considered weak electrogens, meaning that they are capable of EET at a low rate [27]. In this case, EET is mediated by phenazines and pyoverdines [28].…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolkit for a New Species of <em>Pseudomonas</em> Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Preprint

View full text Add to dashboard Cite

Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC's anode. Moreover, Pseudomonas species can grow under low oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species, grown with 1 g.L-1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m-2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium's potential as a synthetic biology non model chassis. Through testing various genetic parts, including constitutive promoters, replication origins, and cargos using pSEVA vectors as a scaffold, we assessed the bacterium's suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

show abstract

Electrobiochemical skills of Pseudomonas aeruginosa species that produce pyocyanin or pyoverdine for glycerol oxidation in a microbial fuel cell

Cited by 13 publications

References 50 publications

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

State of the Art and Environmental Aspects of Plant Microbial Fuel Cells’ Application

Synthetic Biology Toolkit for a New Species of <em>Pseudomonas</em> Promissory for Electricity Generation in Microbial Fuel Cells

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