Electrocatalytic oxidation and determination of dexamethasone at an Fe3O4/PANI–CuII microsphere modified carbon ionic liquid electrode

Fatahi, Azadeh; Malakooti, Reihaneh; Shahlaei, Mohsen

doi:10.1039/c6ra26125f

Cited by 36 publications

(8 citation statements)

References 34 publications

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“… Method Linear dynamic range LOD Applications/Comment Ref. Label-free electrochemical aptasensor 2.5–100 nM 2.12 nM Applied to tab water and drinking water, recovery = 81.5–103.2% This work reverse-phase high-performance liquid chromatography with diode array detection Not report water = 6 ng/mL Feed = 190 ng/g Applied to water and feed for meat-producing animals: recovery = 99.4 ± 1.3% Flumethasone was used as internal standard 3 Electrochemical sensor based Fe 3 O 4 /PANI–Cu II microsphere 0.05 to 30 mM 3.0 nM Applied to human urine and serum samples Recovery = 97.0–102.0% 11 high-performance liquid chromatography with diode array detection Not report 10 ng/mL Applied to human plasma with recovery = 96.96% to 106.07% 27 Hanging mercury drop electrode 25.5–122.3 µM 7.6 µM Applied to drug sample; recovery = 99.8–100% 28 square-wave adsorptive voltammetry 0.0498–0.61 µM 2.54 nM Applied to eye drops, injectable, elixir; recovery = 94.14–112.41% 29 …”

Section: Resultsmentioning

confidence: 99%

“…Therefore, accurate monitoring of the existence and the concentration of DXN in environmental and clinical samples is very important. The concentrations of DXN in real samples were found from sub nanomolar to micromolar range with different matrices 1 , 10 , 11 . Thus, the development of a rapid and sensitive detection method for the identification of DXN is required.…”

Section: Introductionmentioning

confidence: 99%

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Selection of high affinity aptamer-ligand for dexamethasone and its electrochemical biosensor

Mehennaoui

Poorahong

Jiménez

2019

Sci Rep

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A high specificity aptamer-ligand biorecognition and binding system to monitor of dexamethasone (DXN) was developed. The detection principle was based on a label-free electrochemical aptasensor. The selection of the aptamer was successfully performed by the systematic evolution of ligands through exponential enrichment technique (SELEX). From a random library of 1.08 × 10 15 single-stranded DNA, an aptamer designated as DEX04 showed a highest affinity with a dissociation constant of 18.35 nM. It also showed a good conformational change when binding with DXN. In addition, the aptamer DEX04 did not show any cross-reactivity with other commonly used hormones. An impedimetric aptasensor for DXN was then developed by immobilizing DEX04 on a gold electrode. The binding upon to DXN was monitored by following the change in the charge transfer resistance (Rct) of the [Fe(CN) 6 ] 4−/3− redox couple. The aptasensor exhibited a linear range from 2.5 to 100 nM with a detection limit of 2.12 nM. When applied aptasensor to test in water samples, it showed good recovery percentages. The new DXN aptamer can be employed in other biosensing applications for food control and the diagnosis of some diseases in medicine as a cost-effective, sensitive and rapid detection method.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection of high affinity aptamer-ligand for dexamethasone and its electrochemical biosensor

Mehennaoui

Poorahong

Jiménez

2019

Sci Rep

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“…However, these methods are time‐consuming and expensive and need large amounts of highly pure organic solvents, while electrochemical methods are inexpensive, simple and easy to miniaturize for small‐volume samples. In recent years, some studies have been conducted on the electrochemical behavior of dexamethasone using different techniques and different electrodes, each of which has its own advantages and disadvantages .…”

Section: Introductionmentioning

confidence: 95%

Detection of Dexamethasone Sodium Phosphate in Blood Plasma: Application of Hematite in Electrochemical Sensors

2020

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We studied sensor application of a graphene oxide and hematite (α‐Fe2O3/GO) composite electrode well‐characterized by the SEM and XRD. Through differential pulse voltammetry (DPV), oxidation of dexamethasone sodium phosphate (DSP) was studied at the surface of a glassy carbon electrode (GCE) modified with graphene oxide nanosheets (GO) and the α‐Fe2O3/GO composite. The values of the transfer coefficient (α) and the diffusion coefficient (D) of DSP were 0.5961 and 4.71×10−5 cm2 s−1 respectively. In the linear range of 0.1–50 μM, the detection limit (DL) was 0.076 μM. In the second step, a GCE was modified with α‐Fe2O3/GO composite and the DSP measurement step was repeated to analyzed and compare the effects of hematite nanoparticles present on graphene oxide surfaces. According to the results, α and D were 0.52 and 2.406×10−4 cm2 s−1 respectively and the DL was 0.046 μM in the linear range of 0.1–10.0 μM. The sensor is simple, inexpensive and uses blood serum.

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“…These methods include spectrophotometry,[ 5 6 ] kinetic spectrophotometry,[ 7 ] liquid chromatography,[ 8 9 10 ] high-performance liquid chromatography (HPLC),[ 11 12 ] HPLC with mass spectrometry (HPLC/MS),[ 13 14 ] reversed-phase HPLC in combination with other drugs,[ 15 16 ] and electrochemical methods. [ 17 ]…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method

Al-Owaidi

Alkhafaji

Mahood

2021

Journal of Advanced Pharmaceutical Technology &Amp; Research

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Dexamethasone sodium phosphate (DSP) is an ester of dexamethasone with anti-inflammatory action. This study provides new insights to develop a simple, precise, and accurate spectrophotometric method for the quantitative determination of DSP in bulk and pharmaceuticals. The method was validated before being applied to determine the DSP in six pharmaceutical injection forms from different companies. DSP is soluble in phosphate buffer, so it was used as a solvent, and a pH of 6 was found to be suitable for determination purposes. The DSP solution was scanned in the ultraviolet range (200–400 nm) using a double-beam spectrophotometer with a 1-cm quartz cell. The wavelength (λ max) of DSP was set at 242.5 nm, following the Beer–Lambert law for concentrations from 2 to 50 μg/ml. Dexa AIWA (Germany) showed the best results, being very close to the bulk value with no significant variation. Similarly, Dexamed (Cyprus) and HEMAZON (Syria) showed no significant differences from the bulk; however, the three remaining injections, DEXAKAL (India), DEXABRU (India), and DEXARON (China), showed significant variations from the bulk. Estimated limit of detection and limit of quantitation values for DSP were 0.83 and 2.5 μg/ml, respectively, with a regression coefficient of 0.999. Recovery studies were then used to determine the accuracy of the suggested method. The percentage of recovery was found to be 98.58%–102.52%. All results are suggesting a pivotal method for the routine analysis of DSP both in pure form and the commercially pharmaceutical forms.

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Electrocatalytic oxidation and determination of dexamethasone at an Fe₃O₄/PANI–Cu^II microsphere modified carbon ionic liquid electrode

Abstract: A novel, simple, sensitive and selective electrochemical sensor based on an Fe3O4/PANI–CuII microsphere modified carbon ionic liquid electrode is constructed and utilized for the determination of dexamethasone.

Cited by 36 publications

References 34 publications

Selection of high affinity aptamer-ligand for dexamethasone and its electrochemical biosensor

Selection of high affinity aptamer-ligand for dexamethasone and its electrochemical biosensor

Detection of Dexamethasone Sodium Phosphate in Blood Plasma: Application of Hematite in Electrochemical Sensors

Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method

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