Electrocatalytic Oxidation of NADH at Glassy Carbon Electrodes Modified with Transition Metal Complexes Containing 1,10-Phenanthroline-5,6-dione Ligands

Wu, Qin; Maskus, M.; Pariente, F.; Tobalina, F.; Fernandez, V.; Lorenzo, Encarnación; Abruña, Héctor D.

doi:10.1021/ac960395y

Cited by 120 publications

(80 citation statements)

References 38 publications

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“…4). The linear range was wider than that of 3 -50 mM from GCEs modified with transition metal complexes containing 1, 10-Phenanthroline-5, 6-dione ligands [32] and 10 À4 -10 À2 M from enzyme modified by electropolymerization of aminobenzene isomer and PPQ on electrode [33]. The detection limit of 8.0 mM were obtained at a signal to noise ratio of 3 which was lower than 5 Â 10 À4 from electrode modified by PPQ [33] since the large specific surface area and high enzyme loading on the porous materials.…”

Section: Flow Injection Analysis For Nadh Determinationmentioning

confidence: 87%

Detection of NADH and Ethanol at Titanium Containing MCM‐41 with Low Overpotential

Dai

Bao

et al. 2007

Electroanalysis

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Titanium-containing MCM-41 (Ti-MCM-41) modified glassy carbon electrode (GCE) can exhibit an excellent electrocatalytic activity towards the oxidation of b-Nicotinamide adenine dinucleotide (NADH). A dramatic decrease in the overvoltage of NADH oxidation reaction is observed at 0.28 V vs. SCE. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this material. The proposed sensor shows a highly sensitivity, an acceptable reproducibility and a good stability. The linear range of ethanol is 25 -1000 mM and the detection limit is 8.0 mM. Ti-MCM-41 modified electrode not only can be used to detect the concentration of NADH in biochemical reaction, but also as the potential matrix for the construction of dehydrogenases sensor.

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Section: Flow Injection Analysis For Nadh Determinationmentioning

confidence: 87%

Detection of NADH and Ethanol at Titanium Containing MCM‐41 with Low Overpotential

Dai

Bao

et al. 2007

Electroanalysis

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“…Redox mediators used are ferrocene and its derivatives [12], organic dyes (e.g., phenazines, phenothiazines or phenoxazines) [13], quinones [14][15][16] and (Ru, Os, Re) transition metal complexes [17][18][19]. Shortcomings associated with the use of these mediators are still present even though the overvoltage for the electroxidation of NADH is significantly reduced.…”

Section: Introductionmentioning

confidence: 99%

Cobalt Polypyridyl Complexes as Redox Mediators for Lipoamide Dehydrogenase

Colón

Guadalupe

1998

Electroanalysis

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A series of cobalt polypyridyl complexes were synthesized and characterized by cyclic voltammetry in aqueous and acetonitrile solutions. Their mediation ability toward FADH 2 oxidation in a monoenzyme (lipoamide dehydrogenase) and a bienzyme (lipoamide dehydrogenase coupled to lactate dehydrogenase) system was studied. Of all the complexes tested, only the [Co(tpy) 2 ](PF 6 ) 2 , [Co(NH 2 -phen) 3 ](PF 6 ) 2 and [Co(CH 3 -phen) 3 ](PF 6 ) 2 showed a current enhancement indicative of an EC cat mechanism in the presence of lipoamide dehydrogenase and NADH. No cross reactivity was observed for these complexes in the presence of lactate dehydrogenase/ NAD þ and lactate. The results also suggest that factors such as hydrophobic interactions, steric hindrance, H-bonding and driving force are important when designing a redox mediator for a biosensor. Protein and/or cofactor adsorption fouled the electrode surface lowering the current response. A self-contained bienzymatic biosensor exhibited a linear response (peak current versus log [lactate]) for lactate concentrations between 5 M to 5 mM with a lowest detectable lactate concentration of 4.76 M.

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“…Meanwhile, the stability of the Nafion ® coated PtO x /BDD was much higher than that observed on a ethanol biosensor based on the enzymatic activity of immobilized (on a nylon mesh) alcohol dehydrogenase, coupled with the electrocatalytic oxidation of NADH. 51 It is important to point out that the responses for the ethanol oxidation reaction observed in Figure 5 present a similar shape and a reaction onset potential (~ 600 mV vs. HESS) than those previously reported for polycrystalline platinum electrodes (between 550 and 600 mV vs. RHE) [52][53][54] and for Pt particles dispersed on carbon (~ 600 mV vs. HESS), 55 demonstrating that these responses are only due to the Pt catalyst activity on the BDD surfaces as well as the high purity of deposits. On the other hand, the ethanol oxidation reaction in acid media has a onset potential higher than 1000 mV vs. HESS on the IrO 2 /BDD, RuO 2 /BDD and PbO 2 /BDD electrodes (not shown) in a clear indication of their low electrocatalytic activity for such reaction.…”

Section: Resultsmentioning

confidence: 99%

AFM studies and electrochemical characterization of boron-doped diamond surfaces modified with metal oxides by the Sol-Gel method

Suffredini¹,

Salazar‐Banda²,

Okamura³

et al. 2006

J. Braz. Chem. Soc.

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Com o objetivo de aprofundar investigações anteriores, foram realizadas modificações superficiais diretas em eletrodos de diamante dopados com boro (DDB), com óxidos metálicos (PtO x , RuO 2 , IrO 2 e PbO 2 ) e com alguns compósitos mistos, utilizando a técnica Sol-Gel. Estes materiais foram estudados por microscopia de força atômica (MFA) e os resultados indicaram a formação de sítios de deposição heterogênea. Técnicas eletroquímicas foram utilizadas para estabelecer a atividade catalítica para a reação de desprendimento de oxigênio (RDO) e para reação de oxidação de etanol em meio ácido utilizando os compósitos de PtO x e PtO x -RuO 2 . Testou-se ainda a estabilidade de um depósito de PtO x , recoberto por um filme de Nafion ® , por meio de operação contínua de longa duração. Estudos de voltametria cíclica comparativa mostraram que a área superficial ativa dos novos materiais mudou consideravelmente com o método de modificação proposto. O eletrodo de IrO 2 /BDD mostrou o melhor desempenho para a RDO, iniciando o processo de oxidação em ~ 1.4 V vs. HESS, valor este 200 mV menor do que o observado sobre um eletrodo de PtO x /BDD. Foi observada uma pequena perda da atividade do eletrodo de PtO x /BDD (6%) depois de realizada eletrólise de 4 horas, enquanto que 1000 ciclos voltamétricos deixaram a superfície praticamente inalterada. Os estudos realizados mostram que as atividades satisfatórias dos eletrodos preparados pelo método Sol-Gel podem constituir ferramentas interessantes de pesquisa, visando principalmente a utilização destes materiais em aplicações práticas.Continuing previous investigations, direct surface modifications of boron-doped diamond (BDD) electrodes with metal oxides (PtO x , RuO 2 , IrO 2 and PbO 2 ) and with some mixed composites were carried out by the Sol-Gel technique. The materials were studied by atomic force microscopy (AFM) to determine their surface topologies and by electrochemical techniques to establish the catalytic activity towards the oxygen evolution reaction (OER) and also, for the PtO x and PtO xRuO 2 composites, the ethanol oxidation reactions in acid media. The stability of PtO x coating covered by a Nafion ® film was also tested by long-term operation. The AFM results indicated sites of heterogeneous deposition and the electrochemical studies demonstrated that the active surface area changed considerably with the proposed method of modification. The IrO 2 /BDD electrode showed the best performance to the OER with the onset of the oxidation current at ~1.4 V, a value 200 mV lower than for the PtO x /BDD electrode. The enhanced stability of PtO x /BDD electrodes achieved by the application of a Nafion ® film and already reported in acid media was further proved using the ethanol oxidation reaction. Only a small loss of activity (6%) was observed after 4-hours electrolysis while one-thousand voltammetric cycles left the surface practically unchanged. In addition, preliminary studies for the same reaction on PtO x /BDD and PtO x -RuO 2 / BDD electrodes demonstrated the excell...

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Electrocatalytic Oxidation of NADH at Glassy Carbon Electrodes Modified with Transition Metal Complexes Containing 1,10-Phenanthroline-5,6-dione Ligands

Cited by 120 publications

References 38 publications

Detection of NADH and Ethanol at Titanium Containing MCM‐41 with Low Overpotential

Detection of NADH and Ethanol at Titanium Containing MCM‐41 with Low Overpotential

Cobalt Polypyridyl Complexes as Redox Mediators for Lipoamide Dehydrogenase

AFM studies and electrochemical characterization of boron-doped diamond surfaces modified with metal oxides by the Sol-Gel method

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