Electrocatalytic oxidation of NADH based on polyluminol and functionalized multi-walled carbon nanotubes

Abstract

This work presents that the electrocatalytic oxidation of NADH can be enhanced by the hybrid composites of polyluminol and functionalized multi-walled carbon nanotubes (MWCNTs). The hybrid composites can be easily prepared by the electropolymerization of luminol and the adsorption of functionalized MWCNTs. The modified electrode exhibits two redox couples which can show two electrocatalytic peaks at about 0.1 and 0.3 V (vs. Ag/AgCl) to NADH oxidation. The kinetic constant, k(kin), for the electrocatalytic oxidation of NADH, evaluated by chronoamperometry and voltammetry using a rotating disk electrode (RDE), provided values close to 10(5) M(-1) s(-1). At an applied potential of 0.1 V, the sensor provides a linear response range for NADH from 5 × 10(-6) up to 1.5 × 10(-4) M with a sensitivity of 183.9 μA mM(-1) cm(-2), and detection and quantification limits of 0.6 and 5 μM (S/N = 3), respectively.

Single-walled carbon nanotubes covalently functionalized with polytyrosine: A new material for the development of NADH-based biosensors

“…However, there are just few reports about the electrocatalytic oxidation of NADH at electrodes modified with polymers that works themselves as redox mediators, e.g. poly (3,4)-ethylenedioxytiophene (Rajaram et al, 2015), poly-thionine (Li et al, 2013a), poly-xanthurenic acid (Dos-Santos et al, 2010;Lin et al, 2013), poly-luminol (Lin et al, 2012) or pyrocatechol violet (Zhu et al, 2014.…”

Electrochemical behavior and voltammetric determination of dihydronicotinamide adenine dinucleotide using a glassy carbon electrode modified with single-walled carbon nanohorns

“…However, the direct oxidation of NADH at a traditional solid electrode (bare Pt or GCE) encounters some problems, such as large overvoltage and surface fouling associated with the adsorption of reaction products [7]. Consequently, various nanomaterials, including carbon nanotubes (CNTs) [8], carbon nanofibers [9], gold nanoparticles [10], and graphene [11] have been proved to be suitable mediators to detect NADH with low overpotential and increased peak currents.…”

Electrochemical Studies of Inclusion Complex Formed Between Glutathione and β‐cyclodextrin‐modified Carbon Electrodes and its Application for Determination of Glutathione

“…Moreover, the reduction current gradually decreased. This behavior is congruent with an electrocatalytic reaction process , and an electrocatalytic effect of β‐CD in the oxidation of GSH (in solution) has been previously noted . For all electrodes, the current increased at around the same potential, 1.14 V, but the response was better defined for the CPE‐(β‐CD) s electrode.…”