Electrocatalytic reduction of NAD+ at glassy carbon electrode modified with single-walled carbon nanotubes and Ru(III) complexes

Salimi, Abdollah; Izadi, Mohadeseh; Hallaj, Rahman; Soltanian, Saeid; Hadadzadeh, Hassan

doi:10.1007/s10008-008-0583-6

Cited by 19 publications

(9 citation statements)

References 56 publications

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“…50 A number of studies on the utilization of Rh and Ru complexes in the electrochemical regeneration of NAD(P)H have been presented in the literature. [97][98][99][100][101][102] Other electroactive materials, like tetracyanoquinodimethane, tetrathiafulvalene and dimethyl ferrocene, have been proved to act as mediators to modify electrodes for the electrochemical regeneration of coenzymes. 103 Azine dyes and their derivatives, and quinones and their derivatives, have been broadly used as two-electron mediators for the oxidation of NADH.…”

Section: Modification Of Electrodes By Mediatorsmentioning

confidence: 99%

Methods for the regeneration of nicotinamide coenzymes

et al. 2013

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Oxidoreductase is the largest class of enzymes and has broad applications in biotechnology since a number of bioconversions involve oxidation/reduction reactions. Coenzymes are always required in oxidoreductase-catalyzed reactions, where nicotinamide coenzymes, NAD(P)H/NAD(P) + , are the most commonly used. They undergo reactions with substrates in biocatalytic processes by converting into their reductive or oxidative forms. The efficient and economical regeneration of nicotinamide coenzymes is therefore of particular significance for industrial applications due to their high cost and large usage. The principal methods used for the regeneration of nicotinamide coenzymes, including enzymatic, chemical, electrochemical and photochemical regeneration methods are surveyed with emphasis on the crucial issues and the state-of-art research relevant to each method. Screening and improving the performance of the enzymes, designing and implementing efficient regeneration routes as well as retaining/recycling coenzymes are the three key issues for the enzymatic method. Development of efficient catalysts with high selectivity is the top priority of the chemical regeneration method. For the electrochemical regeneration method, improvement of the electrode by modification of either the nano-materials or electron mediators is the major concern. The focus of the photochemical regeneration method lies in the exploitation of efficient visible-light photosensitizers.

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Section: Modification Of Electrodes By Mediatorsmentioning

confidence: 99%

Methods for the regeneration of nicotinamide coenzymes

et al. 2013

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“…It has been reported that Ru(trpy)Cl 3 promotes NADH conversion from NAD+ by mediating electron transfer. 18 It is remarkable that a photosystem composed of Tyr-C7 and ZnDPEG (without Ru(trpy)Cl 3 ) showed better performance than ZnDPEG or the combination of ZnDPEG and Ru(trpy)Cl 3 . Although Tyr-C7 does not have electron mediating properties, the introduction of the Tyr-C7 assembly significantly enhanced the photosynthesis performance of ZnDPEG.…”

Section: Light-harvesting Performance Under Visible Lightmentioning

confidence: 97%

An enzyme-coupled artificial photosynthesis system prepared from antenna protein-mimetic tyrosyl bolaamphiphile self-assembly

2016

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An artificial photosynthesis system coupled with an enzyme was constructed using the nanospherical self-assembly of tyrosyl bolaamphiphiles, which worked as a host matrix exhibiting an antenna effect that allowed enhanced energy transfer to the ZnDPEG photosensitizer. The excited electrons from the photosensitizer were transferred to NAD+ to produce NADH, which subsequently initiated the conversion of an aldehyde to ethanol by alcohol dehydrogenase. Production of NADH and ethanol was enhanced by increasing the concentration of tyrosyl bolaamphiphiles. Spectroscopic investigations proved that the photosensitizer closely associated with the surface of the bolaamphiphile assembly through hydrogen bonds that allowed energy transfer between the host matrix and the photosensitizer. This study demonstrates that the self-assembly of bolaamphiphiles could be applicable to the construction of biomimetic energy systems exploiting biochemical activity.

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“…[10] Similarly, iron nitride nanoparticles/nitrogen doped graphene modified GCE was also used to detect NADH in human blood serum. [14] On the other hand, electrocatalytic reduction of NAD + was studied on single-walled carbon nanotube/ruthenium (III) complex [15] and ruthenium modified electrodes. [16,17] Nevertheless, these methods have shown some limitations such as poor selectivity and complicated electrode modification strategies.…”

Section: Introductionmentioning

confidence: 99%

Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

2020

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Reduced form of β‐nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD+) are the main cofactors involved in more than 300 dehydrogenase reactions. NAD+ is one of the important oxidizing agent for the oxidation of alcohol, aldehyde and ketones. Similarly, NADH has been used for the treatment of Alzheimer and Parkinson's diseases. Herein, we have reported synthesis of graphene flakes by electrochemical exfoliation of graphite in sodium tungstate solution. It was found that tungstate (WO42−) and hydroxyl (OH−) ions were intercalated into graphite layers and enabled the production of graphene flakes. As‐obtained graphene flakes were characterized by UV‐Visible (UV‐Vis), Fourier transform infrared (FT‐IR), Raman, Field emission scanning electron microscopy (FE‐SEM), Energy dispersive X‐ray (EDX) and High‐resolution transmission electron microscopies (HR‐TEM). FT‐IR, Raman and EDX analysis were confirmed that tungstate (WO42−) ions were present on the surface of graphene flakes. Moreover, graphene‐WO42− (Gr−W) dispersion was prepared by probe‐sonication to form a thin‐film on the surface of glassy carbon electrode (Gr−W/GCE). Interestingly, Gr‐W modified GCE reduced the overpotentials of NADH oxidation and NAD+ reduction. This new senor was also showed linear responses for NADH and NAD+ from 10–270 μM and 100–500 μM, respectively. Furthermore, the selectivity of the Gr−W/GCE was tested in the presence of L‐tyrosine, L‐isoleucine, L‐alanine, glutathione, dopamine (DA), ascorbic acid (AA), uric acid (UA), oxalic acid (OA), glucose, hydrogen peroxide (H2O2), acetaminophen (PA), potassium chloride (KCl) and sodium chloride (NaCl). It was found that selective detection of both NADH and NAD+ could be achieved by using Gr−W/GCE. Finally, the real application of the sensor was demonstrated by accurately detecting spiked NADH concentrations in human blood serum. The recovery analysis was also confirmed that Gr−W/GCE could be useful to detect NADH in biological samples.

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Electrocatalytic reduction of NAD+ at glassy carbon electrode modified with single-walled carbon nanotubes and Ru(III) complexes

Cited by 19 publications

References 56 publications

Methods for the regeneration of nicotinamide coenzymes

Methods for the regeneration of nicotinamide coenzymes

An enzyme-coupled artificial photosynthesis system prepared from antenna protein-mimetic tyrosyl bolaamphiphile self-assembly

Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

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Electrocatalytic reduction of NAD+ at glassy carbon electrode modified with single-walled carbon nanotubes and Ru(III) complexes

Cited by 19 publications

References 56 publications

Methods for the regeneration of nicotinamide coenzymes

Methods for the regeneration of nicotinamide coenzymes

An enzyme-coupled artificial photosynthesis system prepared from antenna protein-mimetic tyrosyl bolaamphiphile self-assembly

Selective Electrochemical Sensing of NADH and NAD+ Using Graphene/Tungstate Nanocomposite Modified Electrode

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Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode