Electrochemical acetylcholinesterase biosensor based on ZnO nanocuboids modified platinum electrode for the detection of carbosulfan in rice

Nesakumar, Noel; Sethuraman, Swaminathan; Krishnan, Uma Maheswari; Rayappan, John Bosco Balaguru

doi:10.1016/j.bios.2015.11.010

Cited by 78 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum response appears at pH 7.4. This value corresponds to those reported . Thus, pH 7.4 was used in the subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Compared with such traditional analytical methods as gas chromatography , liquid chromatography , mass spectrometry , enzyme‐linked immune‐adsorbent assays , surface plasmon resonance , and surface‐enhanced Raman scattering , amperometric acetylcholinesterase (AChE) biosensors have aroused more and more attention recently because of their easy operation, high sensitivity, fast response and low cost . However, it is still urgent to develop a highly sensitive AChE biosensor with low detection limit.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Zhao

Han

et al. 2018

Electroanalysis

View full text Add to dashboard Cite

Holey graphene oxide (HGO) has been synthesized by one step hydrothermal method and applied to construct a highly sensitive acetylcholinesterase (AChE) biosensor. The developed system is characterized by using SEM, TEM, nitrogen adsorption‐desorption, Raman, and electrochemical impedance spectroscopy. It is proved that HGO can effectively immobilize AChE and accelerate electron transfer rate. The prepared biosensor AChE/HGO/glass carbon electrode (GCE) shows excellent affinity to the acetylthiocholine chloride (ATCl) with a Michaelis‐Menten constant value of 0.23 mM. Under optimum conditions, AChE/HGO/GCE can effectively detect paraoxon with a linear ranged from 10 to 45 ng/mL and a detection limit of 1.58 ng/mL. Furthermore, the prepared AChE/HGO/GCE biosensor demonstrates excellent stability and reproducibility, which provides a reliable method for the detection of organophosphate pesticides. The developed biosensor is used for detecting paraoxon in real samples of lettuce and cabbage and shows a satisfactory recovery.

show abstract

“…The maximum response appears at pH 7.4. This value corresponds to those reported . Thus, pH 7.4 was used in the subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Zhao

Han

et al. 2018

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…[5] In recent years, electrochemical biosensors based on acetylcholinesterase (AChE) have replaced the aforementioned detection methods, and this method has become one of the promising methods for the detection of pesticides due to the simplicity of the preparation process and its high sensitivity and selectivity for organophosphorus. [6][7][8][9][10][11][12] However, the high cost of enzymatic biosensors and the shortcomings of longterm stability and poor reproducibility also limit their application. [13] In contrast, non-enzymatic sensors are a promising analytical method.…”

Section: Introductionmentioning

confidence: 99%

Free‐enzymatic Indirect Detection of Malathion by SiC@CuO‐NPs Composite Nanomaterial Modified Glassy Carbon Electrode

Bakytkarim¹,

Tursynbolat

Huang

et al. 2021

ChemistrySelect

View full text Add to dashboard Cite

We prepared a highly sensitive electrochemical sensor for the free-enzymatic indirect detection of malathion. The electrochemical sensor was based silicon carbide and copper oxide nanocomposite material. The structural and morphological properties of the SiC@CuO-NPs nanocomposite was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). SiC-NPs has the function of malathion preconcentration and SiC@CuO-NPs nanocomposite was noted to possess high affinity towards malathion, thus the redox reaction of CuO could be hindered. This feature was utilized to design as a simple strategy for electrochemical detection of malathion. Electrochemical detection of malathion was investigated via cyclic voltammetry (CV), differential pulse voltammetry (DPV), respectively. Under the optimized experimental conditions, the inhibition rate displays a good linear relationship with the concentration of malathion, the linear range is 0.03 nM ∼ 3.0 nM, and the detection limit is 0.01 nM (S/N = 3). In addition, the sensor exhibits good selectivity, stability and reproducibility, and has been successfully used in the detection of malathion in actual water samples with recoveries between 99.33 % and 106.6 %, which is satisfactory experimental results.

show abstract

“…Recently, various nanoparticles, functional nanomaterials, nanoporous materials, nanocomposites etc have been used as transducing elements in enzyme-based electrochemical biosensors to detect OPs. For example, a platinum working electrode was modified with ZnO nanoparticles in order to detect carbosulfan 21 , poly-(amidoamine) with cystamine core immobilized with AChE enzyme was developed for the detection of carbaryl 22 and nanocomposite composed of multi-walled carbon nanotubes, tin oxide, and chitosan constructed to detect chlorpyrifos 23 . Metal Organic Frameworks (MOFs) are currently being explored for their potential use as a transducing element for ultra-sensitive detection of OPs.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things

Nagabooshanam

Roy

Mathur

et al. 2019

Sci Rep

View full text Add to dashboard Cite

An Electrochemical micro Analytical Device (EµAD) was fabricated for sensitive detection of organophosphate pesticide chlorpyrifos in the food chain. Gold microelectrode (µE) modified with Zinc based Metal Organic Framework (MOF-Basolite Z1200) and Acetylcholinesterase (AChE) enzyme served as an excellent electro-analytical transducer for the detection of chlorpyrifos. Electrochemical techniques such as Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) were performed for electrochemical analysis of the developed EµAD. The sensor needs only 2 µL of the analyte and it was tested within the linear range of 10 to 100 ng/L. The developed EµAD’s limit of detection (LoD) and sensitivity is 6 ng/L and 0.598 µ A/ng L−1/mm2 respectively. The applicability of the device for the detection of chlorpyrifos from the real vegetable sample was also tested within the range specified. The fabricated sensor showed good stability with a shelf-life of 20 days. The EµAD’s response time is of 50 s, including an incubation time of 20 s. The developed EµAD was also integrated with commercially available low-cost, handheld potentiostat (k-Stat) using Bluetooth and the results were comparable with a standard electrochemical workstation.

show abstract

Electrochemical acetylcholinesterase biosensor based on ZnO nanocuboids modified platinum electrode for the detection of carbosulfan in rice

Cited by 78 publications

References 23 publications

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Free‐enzymatic Indirect Detection of Malathion by SiC@CuO‐NPs Composite Nanomaterial Modified Glassy Carbon Electrode

Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things

Contact Info

Product

Resources

About