Electrochemical analysis of copper ion using a Gly–Gly–His tripeptide modified poly(3-thiopheneacetic acid) biosensor

Lin, Meng; Cho, M.S.; Choe, Wonyoung; Lee, Y.

doi:10.1016/j.bios.2009.05.035

Cited by 67 publications

(19 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The peptide Gly–Gly–His (Figure 1) is known to bind copper ions with high affinity. A large range of association constants (10 7 –10 16 ) can be found in literature [15,31,32,33,34]. The selectivity of the GGH peptide towards Cu 2+ has been shown in literature [35,36].…”

Section: Introductionmentioning

confidence: 99%

Detection of Cu2+ Ions with GGH Peptide Realized with Si-Nanoribbon ISFET

Synhaivska

Mermoud

Baghernejad

et al. 2019

Sensors

View full text Add to dashboard Cite

The presence of heavy metal ions such as copper in the human body at certain concentrations and specific conditions can lead to the development of different diseases. The currently available analytical detection methods remain expensive, time-consuming, and often require sample pre-treatment. The development of specific and quantitative, easy-in-operation, and cost-effective devices, capable of monitoring the level of Cu2+ ions in environmental and physiological media, is necessary. We use silicon nanoribbon (SiNR) ion-sensitive field effect transistor (ISFET) devices modified with a Gly–Gly–His peptide for the detection of copper ions in a large concentration range. The specific binding of copper ions causes a conformational change of the ligand, and a deprotonation of secondary amine groups. By performing differential measurements, we gain a deeper insight into the details of the ion–ligand interaction. We highlight in particular the importance of considering non-specific interactions to explain the sensors’ response.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of Cu2+ Ions with GGH Peptide Realized with Si-Nanoribbon ISFET

Synhaivska

Mermoud

Baghernejad

et al. 2019

Sensors

View full text Add to dashboard Cite

show abstract

“…According to Irving–Williams series, Cu(II) complexes are the most stable complexes compared with other divalent first-row transition-metal ions when the major coordination sites are N atoms . GGH, a peptide Cu chelator, has been used in biosensors to detect Cu ions, , and the GGH-Cu 2+ complex has been reported as a critical unit to test S 2– in water by fluorescent measurements . Therefore, GGH was selected as the Cu ion chelator in this study.…”

mentioning

confidence: 99%

Tripeptide GGH as the Inhibitor of Copper-Amyloid-β-Mediated Redox Reaction and Toxicity

Zhang

Wang

et al. 2016

ACS Chem. Neurosci.

View full text Add to dashboard Cite

The Aβ complexes of some redox-active species, such as Cu, cause oxidative stress and induce severe toxicity by generating reactive oxygen species (ROS). Thus, Cu chelation therapy should be considered as a valuable strategy for the treatment of Alzheimer's disease (AD). However, more attention should be paid to the specific chelating ability of these chelating agents. Herein, a tripeptide GGH was used to selectively chelate the Cu(2+) in Aβ-Cu complex in the presence of other metal ions (e.g., K(+), Ca(2+), Ni(2+), Mg(2+), and Zn(2+)) as shown by isothermal titration calorimetry results. GGH decreased the level of HO(•) radicals by preventing the formation of intermediate Cu(I) ion. Thus, the Cu species completely lost its catalytic activity at a superequimolar GGH/Cu(II) ratio (4:1) as observed by UV-visible spectroscopy, coumarin-3-carboxylic acid fluorescence, and BCA assay. Moreover, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay indicates that GGH increased PC-12 cell viability from 36% to 63%, and neurotoxicity partly triggered by ROS decreased. These results indicate potential development of peptide chelation therapy for AD treatment.

show abstract

“…The traditional detection of Cu 2+ relies on atomic absorption spectroscopy (AAS) [ 9 , 10 , 11 ], inductively coupled plasma mass spectroscopy (ICP-MS) [ 12 ], and high-performance liquid chromatography (HPLC) [ 13 ], where bulky equipment, complicated sample preparation, and long testing times are inevitable. To simplify the process, many analytical methods have been developed based on colorimetry [ 14 , 15 , 16 ], electrochemistry [ 17 , 18 ], and fluorescence [ 19 , 20 ]. Among them, some researchers have achieved Cu 2+ detection based on the oxidation-reduction property of Cu 2+ , which can produce hydroxyl radicals [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Visual Quantitation of Copper Ions Based on a Microfluidic Particle Dam Reflecting the Cu(II)-Catalyzed Oxidative Damage of DNA

Cui

Chen

2021

Biosensors

View full text Add to dashboard Cite

Due to the use of copper water pipes and the discharge of industrial wastewater, contamination of copper ions in drinking water has become a severe hazard globally. To routinely check water safety on a daily basis, easy-to-use platforms for quantitative analysis of trace amounts of copper ions (Cu2+) in drinking water is needed. Here, we report microfluidic particle accumulation integrated with a Cu(II)-catalyzed Fenton reaction for visual and quantitative copper ion detection. Microparticles (MMPs) and polystyrene microparticles (PMPs) are connected via a single strand DNA, MB155. However, when Cu2+ is present, MB155 is cleaved by hydroxyl free radicals (•OH) produced from Cu2+/hydrogen peroxide (H2O2) Fenton reactions, causing an increased amount of free PMPs. To visually count them, the particle solution is loaded onto a microfluidic chip where free MMPs and MMPs–MB155–PMPs can be collected by the magnetic separator, while the free PMPs continue flowing until being accumulated at the particle dam. The results showed a good linear relationship between the trapping length of PMP accumulation and the Cu2+ concentration from 0 to 300 nM. A limit of detection (LOD) of 70.1 nM was achieved, which is approximately 449 times lower than the 2 × 103 μg·L−1 (~31.5 μM) required by the World Health Organization (WHO). Moreover, the results showed high selectivity and good tolerance to pH and hardness, indicating compatibility for detection in tap water, suggesting a potential platform for the routine monitoring of copper contamination in drinking water.

show abstract

Electrochemical analysis of copper ion using a Gly–Gly–His tripeptide modified poly(3-thiopheneacetic acid) biosensor

Cited by 67 publications

References 37 publications

Detection of Cu2+ Ions with GGH Peptide Realized with Si-Nanoribbon ISFET

Detection of Cu2+ Ions with GGH Peptide Realized with Si-Nanoribbon ISFET

Tripeptide GGH as the Inhibitor of Copper-Amyloid-β-Mediated Redox Reaction and Toxicity

Visual Quantitation of Copper Ions Based on a Microfluidic Particle Dam Reflecting the Cu(II)-Catalyzed Oxidative Damage of DNA

Contact Info

Product

Resources

About