Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification

Ma, Jianfeng; Gong, Lingbo; Cen, Yingying; Lin, Feng; Su, Yan; Liu, Xingfen; Chao, Jie; Wan, Ying; Su, Shao; Wang, Lianhui

doi:10.1016/j.cclet.2022.108012

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…14−17 Enzyme-free or enzyme-assisted isothermal amplification strategies are especially popular. 18,19 For example, rolling circle amplification (RCA) generates DNA/RNA structures with unique advantages of high efficiency and enhanced targeting ability. 20 Hou et al utilized RCA to fabricate a multiregion linear DNA walker and achieved highly sensitive detection of target miRNA.…”

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confidence: 99%

“…Microarrays suffer from limited discrimination capability between closely related miRNA sequences . Currently, a number of other sensing strategies have been proposed with the techniques of electrochemistry, fluorescence, surface-enhanced Raman scattering (SERS), and so on. − Enzyme-free or enzyme-assisted isothermal amplification strategies are especially popular. , For example, rolling circle amplification (RCA) generates DNA/RNA structures with unique advantages of high efficiency and enhanced targeting ability . Hou et al utilized RCA to fabricate a multiregion linear DNA walker and achieved highly sensitive detection of target miRNA .…”

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confidence: 99%

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Quantification of Multiplex miRNAs by Mass Spectrometry with Duplex-Specific Nuclease-Mediated Amplification

Qian

Tang

2023

Anal. Chem.

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Early quantification of multiplex biomarkers such as microRNAs (miRNAs) is critical during disease pathologic development and therapy. To tackle challenges of low abundance and multiplexing, we herein report a mass-encoded biosensing approach with duplex-specific nuclease (DSN) mediated signal amplification. Magnetic Fe3O4 cores are coated with small gold nanoparticles (AuNPs), which are applied to achieve facile DNA immobilization subsequent separation. This biosensor integrates multiple mass reporters corresponding to different targets (five miRNAs as examples). Due to the excellent resolution of mass spectrometry, these targets can be successfully distinguished in a single spectrum. Wide detection ranges from 10 fM to 1 nM are achieved, and the limits of detection are estimated to be 10 fM. High selectivity is promised due to the enzyme activity of DSN, and practical application in human serum samples performs satisfactorily. The number of targets to be tested can be further expanded by designing different specific mass tags in theory. Therefore, the proposed method can be utilized as an important and valuable tool to quantify multiplex miRNAs for disease screening as well as biomedical investigations.

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confidence: 99%

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confidence: 99%

Quantification of Multiplex miRNAs by Mass Spectrometry with Duplex-Specific Nuclease-Mediated Amplification

Qian

Tang

2023

Anal. Chem.

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“…4 shows the electrochemical detection of miRNA based on triple signal amplification based on the hybrid chain reaction. 67 The capture hairpin probe is connected to the electrode surface by the Au–S bond. HCR amplification is triggered in the presence of target miRNA, and target miRNA-21 is released by strand replacement reaction.…”

Section: Signal Amplification and Amplification Strategymentioning

confidence: 99%