Electrochemical and Optical Biosensors for the Detection of Campylobacter and Listeria: An Update Look

Abstract

Foodborne safety has aroused tremendous research interest in recent years because of a global public health problem. The rapid and precise detection of foodborne pathogens can reduce significantly infection diseases and save lives by the early initiation of an effective treatment. This review highlights current advances in the development of biosensors for detection of Campylobacter spp. and Listeria monocytogenes that are the most common causes of zoonosis. The consumption of pathogen contaminated food is responsible for humans hospitalization and death. The attention focused on the recognition elements such as antibodies (Ab), DNA probes and aptamers able to recognize cells, amplicons, and specific genes from different samples like bacteria, food, environment and clinical samples. Moreover, the review focused on two main signal-transducing mechanisms, i.e., electrochemical, measuring an amperometric, potentiometric and impedimetric signal; and optical, measuring a light signal by OLED (Organic Light Emitting Diode), SPR (Surface Plasmon Resonance), and Optical fiber. We expect that high-performance of devices being developed through basic research will find extensive applications in environmental monitoring, biomedical diagnostics, and food safety.

Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

“…Biosensors attract considerable attention because of their simplicity, sensitivity and rapidity ( Figure 2). Electrochemical biosensors are the most rapidly growing compared to other methods, because they are low cost, sensitive and easy to miniaturize [93][94][95]. In 2012, Bruno and Carrillo reported an aptameric sequence, which specifically binds to spores of B. anthracis and B. cereus [96].…”

Sensitive Detection of E. coli in Artificial Seawater by Aptamer-Coated Magnetic Beads and Direct PCR

“…For instance, optical biosensors that use filters and camera enable to detect bacteria in water by fluorescence or hemiluminescence [12][13][14][15]. In the literature, electrochemical sensors are proposed that detect pathogens thanks to the current or potential variations that occur upon pathogen or its biomarker interaction with an electrode [16][17][18][19][20][21]. Among various detection methods, the most robust are those based on ELISA immunological tests and on PCR-based molecular tests [22].…”

Highly Sensitive Detection ofCampylobacter spp.in Chicken Meat using a Silica Nanoparticle Enhanced Dot Blot DNA Biosensor

“…Identification and quantification of Campylobacter spp. rely on conventional, culturebased methods, and bacterial phenotypical characterization (Vizzini et al 2019).…”

“…Biosensors for detection of Campylobacter show marked advantages over traditional methods in terms of rapidity, facility of utilization and cost-effectiveness (Manzano et al 2015;Masdor et al 2016;Morant-Miñana and Elizalde 2015;Vidic et al 2017;Vidic et al 2019;Vizzini et al 2019;Yang et al 2013). However, today no commercial biosensor is available to detect Campylobacter in food matrices, mainly because of the difficulty to reach high sensitivity.…”