Electrochemical and spectrophotometric characterisation of protein kinase inhibitor and anticancer drug danusertib

Popa, Oana M.; Diculescu, Victor C.

doi:10.1016/j.electacta.2013.09.017

Cited by 17 publications

(8 citation statements)

References 29 publications

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“…The expected mechanism behind the generated irreversible oxidation current at the higher potential is the loss of an electron in the piperazine ring as reported for other piperazine‐containing compounds . Thus, the deprotonated piperazine functional group may be regarded as the electroactive form.…”

Section: Resultsmentioning

confidence: 99%

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A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

et al. 2016

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For the first time, the use of carbon nanotubes was exploited for the development of a sensitive electrochemical method for determination of the newly antifungal posaconazole (POS). The electrochemical activity of POS was investigated at the surface of multi‐walled nanotubes (MWNTs) modified electrode. The cyclic voltammograms showed a sharp oxidation peak at potential around 671 mV vs. Ag/AgCl. To reach the assay optimization, factors affecting the method sensitivity have been investigated, such as types of carbon nanotubes and its concentration in the electrode matrix, type of supporting electrolyte, pH, accumulation time and scan rate. A good linear relationship was obtained within the concentration range from 32–1280 ng/ml with the limit of detection and quantification of 11 ng/ml and 33 ng/ml, respectively. The proposed method was successfully applied for the determination of POS in its commercial dosage form, spiked human plasma samples, and dried blood spots. The in vivo results obtained were also used to study the pharmacokinetics of POS in human plasma. The results obtained were validated and found to be in accordance with those obtained by the reference methods.

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Section: Resultsmentioning

confidence: 99%

“…To decrease the volume of blood sampling, SPE was used for the determination of the drug in human plasma and DBS after sample pretreatment. The results obtained by the proposed method were compared with those obtained from the reported methods ,, respectively according to t‐test and F‐test (Table ).…”

Section: Resultsmentioning

confidence: 99%

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

et al. 2016

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“…Longer incubation times would cause the oxidation peak to reduce where intercalation of the drug with DNA will take over as the main interaction mechanism. [29][30][31] Figure 5B shows a linear regression curve obtained (r 2 = 0.99) for the detection of ciprofloxacin in acetate buffer (background measurement was subtracted from each reading) with a wide linear range of 0.1 -100 μM and a detection limit of 0.1 μM . The limit of detection in this investigation, which corresponds to the amount of ciprofloxacin that can be truly detected in a sample solution, 32 was acquired by visual evaluation.…”

Section: Analytical Performance Of Dna Based Biosensormentioning

confidence: 99%

A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene

Lim

Ahmed

2016

ANAL. SCI.

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In this work we exploited the electrostatic interaction of double stranded DNA (dsDNA) with drug components to construct a simple, but highly sensitive, DNA-electrochemical sensor for detecting ciprofloxacin. The following straightforward three-step procedure was performed to determine ciprofloxacin: (i) dsDNA-layer immobilization on the surface of the working graphene-modified screen-printed carbon electrode; (ii) dsDNA-ciprofloxacin interaction for 2 min; and (iii) electrochemical measurement using square-wave voltammetry. An increased oxidation of the guanine component was observed, at +1.0 V, as a result of the electrostatic interaction of positively charged ciprofloxacin with the negatively charged nucleic acid sugar phosphate. Based on the International Conference on Harmonization Guidelines, a linear relationship between the guanine oxidation peak and ciprofloxacin concentration (0.1 to 100 μM) was obtained with a detection limit of 0.1 μM. Our developed sensor is straightforward to construct and use, requiring no multi-step time-consuming preconditioning of electrodes. It is highly sensitive and selective in the detection of ciprofloxacin, and has the potential to be useful in the future fabrication of rapid and portable on-site food safety analysis devices.Keywords Ciprofloxacin, DNA electrochemical biosensor, graphene, screen-printed electrodes

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“…6B) for SNPC using the method reported in literature. 36 The 2 nd oxidation signal of SNPC showed saturation at 0.7 mM due to the possible adsorption of the oxidation product formed around 0.3 V. Therefore, the peak at 0.3 V was used for analytical determination. constant was determined at different temperatures for the calculation of G # was then calculated.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, Spectroscopic Characterization, pH Dependent Electrochemistry and Computational Studies of Piperazinic Compounds

Parveen

Shah

Khan

et al. 2014

J. Electrochem. Soc.

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This work presents the synthesis, redox behavior and spectroscopic characterization of two novel compounds Sodium 4-(3-methoxyphenyl) piperazine-1-carbodithioate and sodium 4-(4-nitrophenyl) piperazine-1-carbodithioate. Pulse voltammetric techniques were utilized to determine the number of electrons involved in the oxidation and/or the reduction step and to ensure the nature of the redox processes. The pH dependent redox mechanistic pathways of the compounds were proposed on the basis of electrochemical and computational results. Different thermodynamic parameters like G # and H # revealed that electrode processes are non-spontaneous and endergonic in nature. Increase in S # values at higher temperature indicated the randomness of the electrode reactions at higher temperatures. Limits of detection and quantification were determined by square wave voltammetry due to its high sensitivity and fast speed. Ionization energy, electron affinity, dipole moment and charge distribution on atoms were computationally determined. Acid-base dissociation constant (pKa) values of the compounds evaluated by voltammetry and electronic spectroscopy were found comparable. Molecular architectures bearing piperazine group have gained the utmost attention due to their vast applications in medical and industrial fields. Piperazines have special importance among nitrogen containing heterocyclic compounds due to their hydrogen bonding ability that make such compounds very specific for the generation of supramolecular structures.1 Piperazine can exhibit chair or boat conformation with chair form being more stable by 17.2 kJ/mol.2 Compounds containing piperazine ring are used as raw materials for the synthesis of epoxy resins, antioxidants, urethane catalysts, insecticides and accelerators for rubber.3 Such compounds possess versatile binding properties and act as potent and selective ligands for different biological activities. The attachment of piperazine to porphrin ring has been reported to enhance the anticancer activity and play the role of sensitizer in photodynamic therapy.4-8 Piperazine derivatives show good activities against chloroquine resistant strain of Plasmodium falciparum in vitro with low toxicity against murine monocyte/macrophage cells.9 Such compounds are also used as chelating agents due to their ability of forming complex with metal ions. Free radicals formation in many bioorganic redox processes may result in oxidation of lipids, proteins, or DNA and thus initiate cancer, cardiovascular, autoimmune, inflammatory and age-related degenerative brain diseases. So, piperazine as reducing agent is gaining mounting attention due to free radicals scavenging ability. 10 Dithio ligands have been documented to play vital roles in numerous biological and non-biological processes.11-13 Dithiocarbamate (DTC) derivatives are used as organic intermediates, vulcanizing agents, rubber additives and fungicides.14 DTC a putative immunomodulator plays pivotal roles in agriculture and enhances immune response in AIDS treatment. 15 Comple...

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Electrochemical and spectrophotometric characterisation of protein kinase inhibitor and anticancer drug danusertib

Cited by 17 publications

References 29 publications

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

A Disposable Carbon Nanotubes‐screen Printed Electrode (CNTs‐SPE) for Determination of the Antifungal Agent Posaconazole in Biological Samples

A Simple DNA-based Electrochemical Biosensor for Highly Sensitive Detection of Ciprofloxacin Using Disposable Graphene

Synthesis, Spectroscopic Characterization, pH Dependent Electrochemistry and Computational Studies of Piperazinic Compounds

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