Electrochemical aptamer-based biosensors as potential tools for clinical diagnostics

Jarczewska, Marta; Górski, Łukasz; Malinowska, Elżbieta

doi:10.1039/c6ay00499g

Cited by 85 publications

(52 citation statements)

References 96 publications

(95 reference statements)

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“…Composites of graphene oxide with Bi 2 S 3 nanorods were used for the electrochemical determination of dopamine [13], and arrays of cylindrical gold nanoelectrodes could be used for both the detection of dopamine and for the immobilization and growth of human neural cells [14]. Many other systems based on organic and inorganic materials have been reported for the sensing of dopamine [2,3].Synthetic bio-receptors such as aptamers appeared to be highly attractive for selective detection of dopamine [15][16][17]. Aptamers are artificial, relatively short single strand DNA or RNA oligomers having a particular sequence of nucleotides designed to accommodate a target molecule, e.g., dopamine.…”

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confidence: 99%

“…On binding their target, the aptamers adopt a conformational change from a relatively simple chain structure into a complex 3D shape that wraps around the desired target. A combination of high selectivity of biological systems with the stability and possibly for large scale synthesis of artificial systems make aptamers especially attractive for a wide range of applications; several reviews on the properties and applications of aptamers have been published recently [15][16][17].Several research groups have studied the use of aptamers as the recognition elements in dopamine sensors. A 58-nucleotide aptamer could be combined with gold nanoparticles to give a colorimetric sensor for dopamine where the process of aptamer-dopamine binding facilitates aggregation of gold nanoparticles and subsequent color change [18]; a linear detection range of 5.4 × 10 −7 M to 5.4 × 10 −6 M and a detection limit of 3.6 × 10 −7 M were reported.…”

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Electrochemical Aptasensor for Detection of Dopamine

et al. 2020

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This work presents a proof of concept of a novel, simple, and sensitive method of detection of dopamine, a neurotransmitter within the human brain. We propose a simple electrochemical method for the detection of dopamine using a dopamine-specific aptamer labeled with an electrochemically active ferrocene tag. Aptamers immobilized on the surface of gold screen-printed gold electrodes via thiol groups can change their secondary structure by wrapping around the target molecule. As a result, the ferrocene labels move closer to the electrode surface and subsequently increase the electron transfer. The cyclic voltammograms and impedance spectra recorded on electrodes in buffer solutions containing different concentration of dopamine showed, respectively, the increase in both the anodic and cathodic currents and decrease in the double layer resistance upon increasing the concentration of dopamine from 0.1 to 10 nM L −1 . The high affinity of aptamer-dopamine binding (KD ≈ 5 nM) was found by the analysis of the binding kinetics. The occurrence of aptamer-dopamine binding was directly confirmed with spectroscopic ellipsometry measurements.Chemosensors 2020, 8, 28 2 of 11 done on the electrochemical detection of dopamine and other neurotransmitters [2,3]. Dopamine can be very easily detected in an aqueous solution by electrochemical methods such as cyclic voltammetry since dopamine undergoes electrochemical oxidation. However, a major issue is the selectivity since complex matrices such as blood contain a range of other oxidizable compounds such as urate or ascorbate which would also generate signals, leading to inaccurate readings. Selectivity towards dopamine is therefore required.A wide range of electrode materials have been proposed to increase the selectivity of dopamine detection [2,3]. A few recent examples include the use of materials such as Nafion combined with graphite [4] or multi-walled carbon nanotubes [5] to improve the selectivity towards dopamine. Other researchers have utilized graphene-modified screen-printed electrodes [6] or graphene aerogels [7] as substrates for the selective determination of dopamine. Composites of carbon nanotubes and graphene oxide could be used to determine dopamine, nitrate, ascorbate, and urate [8]. Metal nanoparticles have also been utilized, for example palladium nanoparticles combined with graphene [9], which were used as a base of electrochemical determination of dopamine whereas a gold nanoparticle/DNA/polymer composite could be used for the simultaneous determination of dopamine, uric acid, guanine, and adenine [10]. Composites of gold nanoparticles with conducting polymers have also been used for the selective determination of dopamine [11]. Boron-doped carbon nanotubes could be used to determine levels of dopamine and ephedrine in the presence of urate [12]. Composites of graphene oxide with Bi 2 S 3 nanorods were used for the electrochemical determination of dopamine [13], and arrays of cylindrical gold nanoelectrodes could be used for both the detection of dopamine an...

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Electrochemical Aptasensor for Detection of Dopamine

et al. 2020

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“… GONC is first modified with OTA‐apt. In the presence of OTA, its specific binding to OTA‐apt causes the aptamer conformational change from a random coil to an antiparallel G‐quadruplex . This allows the partial detachment of the stable antiparallel G‐quadruplex from GONC surface.…”

Section: Resultsmentioning

confidence: 99%

“…In the presence of OTA, its specific binding to OTA-apt causes the aptamer conformational change from a random coil to an antiparallel G-quadruplex. [12] This allows the partial detachment of the stable antiparallel G-quadruplex from GONC surface. 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 contrast, upon incubation with OTA a decrease in R CT values is generally observed, possibly due to a partial release of OTAaptamers from the surface of the OTA-apt modified GONCs.…”

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confidence: 99%

The Role of Surface Chemistry in Impedimetric Aptasensing

2018

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Surface chemistry is a key parameter in the choice of proper materials for electrochemical detection. It has been previously shown that the presence of oxygen containing groups (OCGs) on the surface of graphene oxide (GO) can be both effective and detrimental. This poses a question when GO materials are used as electrochemical platforms for biosensing. In this work, we study how the surface chemistry of graphene oxide nanocolloids (GONCs) affects the impedimetric biosensing of ochratoxin A (OTA), in terms of immobilization of biorecognition element and detection step. OCGs on GONCs were tuned by applying increasing reduction potentials from −0.3 V to −1.2 V, resulting in GONC platforms with decreasing amounts of oxygen functionalities. It was discovered that the sensitivity of biosensing is correlated to the residual amount of OCGs on GO surface. For a more detailed investigation, three representative materials, namely unreduced GONCs, as well as GONCs reduced at potentials of −0.8 V and −1.2 V were chosen. Results were compared in terms of calibration sensitivity, selectivity and reproducibility of the impedimetric response. GONCs reduced at −1.2 V have shown the best electroanalytical response for the impedimetric detection of OTA. These findings are anticipated to contribute to the design of novel biosensors, whereby an optimized platform is employed for the immobilization of the biorecognition element.

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“…This stability facilitates derivatization, purification and immobilization on different surfaces without losing their biorecognition abilities. Although aptamers have been successfully employed in biosensing applications with different transducers (optical and piezoelectric transducers, for instance), its use in electrochemical biosensing applications appears to be more advantageous due to the low limit of detection achieved by this technique, in addition to its high selectivity, simplicity and possibility of miniaturization . In electrochemical aptasensors, proper attachment of the aptamer to the electrode surface is very important.…”

Section: Aptamer‐based Electrochemical Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors in Point‐of‐Care Devices: Recent Advances and Future Trends

et al. 2017

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The use of biosensors in point‐of‐care (POC) testing devices has attracted considerable attention in the past few years, mainly because of their high specificity, portability, and relatively low cost. Coupling these devices with miniaturized electrochemical transducers has shown great potential toward simple, rapid, and cost‐effective analysis that can be performed in the field, especially for healthcare, environmental monitoring, and food quality control. For this reason, the number of publications in this field has grown exponentially over the past decade, making it a trending topic in current research. Although great improvement has been achieved in the field of electrochemical biosensing, there are still some challenges to overcome, especially concerning the improvement of sensing materials and miniaturization. In this Review, we summarize some of the most recent advances achieved in POC electrochemical biosensor applications, focusing on new materials and modifiers for biorecognition developed to improve sensitivity, specificity, stability, and response time.

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Electrochemical aptamer-based biosensors as potential tools for clinical diagnostics

Abstract: A schematic representation of the aptamer–antibody sandwich assay.

Cited by 85 publications

References 96 publications

Electrochemical Aptasensor for Detection of Dopamine

Electrochemical Aptasensor for Detection of Dopamine

The Role of Surface Chemistry in Impedimetric Aptasensing

Electrochemical Biosensors in Point‐of‐Care Devices: Recent Advances and Future Trends

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