Electrochemical Aptasensor for Zearalenone Based on DNA Assembly and Exonuclease III as Amplification Strategy

Abstract

A sensitively electrochemical aptasensor was developed to detect zearalenone, utilizing DNA assembly based on hybridization chain reaction to amplify the signal current and exonuclease III to reduce the background current. The linear range 5.0×10−5 ng/mL‐50 ng/mL, and the limit of detection is 0.013 pg/mL. The fabricated aptasensor showed the high specificity toward aflatoxin B1 (AFB1), fumonisin B1 (FB1) and ochratoxin A (OTA), good repeatability and reproducibility. In addition, the average recoveries of spiked corn and beer samples were in the range of 89 % to 102 %. The established method is of great significance in the field of food safety detection.

Recent Advances in Recognition Receptors for Electrochemical Biosensing of Mycotoxins—A Review

“…Wei et al [ 52 ] fabricated a new electrochemical aptasensor for the sensitive detection of zearalenone by combining hybridization chain reaction, methylene blue, and exonuclease III for signal amplification and minimizing background current. Exo III operates on double-stranded DNA (dsDNA) and does not require a particular recognition sequence; it may progressively remove single nucleotides along the 3′-5′ direction.…”

Electrochemical sensor propelled by exonuclease III for highly efficient microRNA-155 detection

“…Electrochemical aptasensors have been developed based on Exo-III-aided catalytic reaction and HCR, such as the detection of Hg 2+ , 42,43 and zearalenone. 44 Luo reported an electrochemical sensing method to detect DNA based on coupled Exo-III catalysis and HCR, with a detection limit as low as 0.10 fM. 45 In this paper, we designed an electrochemical sensor based on an Exo-III-assisted amplification reaction to detect miRNA-155.…”