Electrochemical attack and corrosion of platinum electrodes in dielectrophoretic diagnostic devices

Heineck, Daniel; Sarno, Benjamin; Kim, Sejung; Heller, Michael

doi:10.1007/s00216-020-02607-7

Cited by 7 publications

(8 citation statements)

References 30 publications

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“…It also mitigates against ion screening of the particle, electrolysis of the media, and electroosmotic flow. Frequency choice may also control the electrochemistry in some cases [22]. Additional factors may affect the electrical properties of the constituents as well, such as the local ion concentration in the medium or temperature of the system.…”

Section: Background/theorymentioning

confidence: 99%

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Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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The 20th century has seen tremendous innovation of dielectrophoresis (DEP) technologies, with applications being developed in areas ranging from industrial processing to micro‐ and nanoscale biotechnology. From 2010 to present day, there have been 981 publications about DEP. Of over 2600 DEP patents held by the United States Patent and Trademark Office, 106 were filed in 2019 alone. This review focuses on DEP‐based technologies and application developments between 2010 and 2020, with an aim to highlight the progress and to identify potential areas for future research. A major trend over the last 10 years has been the use of DEP techniques for biological and clinical applications. It has been used in various forms on a diverse array of biologically derived molecules and particles to manipulate and study them including proteins, exosomes, bacteria, yeast, stem cells, cancer cells, and blood cells. DEP has also been used to manipulate nano‐ and micron‐sized particles in order to fabricate different structures. The next 10 years are likely to see the increase in DEP‐related patent applications begin to result in a greater level of technology commercialization. Also during this time, innovations in DEP technology will likely be leveraged to continue the existing trend to further biological and medical‐focused applications as well as applications in microfabrication. As a tool leveraged by engineering and imaginative scientific design, DEP offers unique capabilities to manipulate small particles in precise ways that can help solve problems and enable scientific inquiry that cannot be addressed using conventional methods.

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Section: Background/theorymentioning

confidence: 99%

“…Heineck et al. explored the limits of DEP device function using two different architectures, with the end point being either electrode failure or sample degradation [22]. Sinusoidal signals were tested across different frequencies and voltages.…”

Section: Biological Applicationsmentioning

confidence: 99%

Dielectrophoresis: Developments and applications from 2010 to 2020

et al. 2020

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“…These reactions degrade the integrity of electrodes, ultimately making them unusable for dielectrophoretic experiments. A recent study by Heineck et al [95] reported on the interactions of sputtered platinum electrodes with plasma and PBS buffers. The study was conducted to determine the voltages and frequency regions at which these electrodes could be safely used in experiments.…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021

et al. 2021

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Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021Dielectrophoresis is a well-understood phenomenon that has been widely utilized in biomedical applications. Recent advancements in miniaturization have contributed to the development of dielectrophoretic-based devices for a wide variety of biomedical applications. In particular, the integration of dielectrophoresis with microfluidics, fluorescence, and electrical impedance has produced devices and techniques that are attractive for screening and diagnosing diseases. This review article summarizes the recent utility of dielectrophoresis in assays of biomarker detection. Common screening and diagnostic biomarkers, such as cellular, protein, and nucleic acid, are discussed. Finally, the potential use of recent developments in machine learning approaches toward improving biomarker detection performance is discussed. This review article will be useful for researchers interested in the recent utility of dielectrophoresis in the detection of biomarkers and for those developing new devices to address current gaps in dielectrophoretic biomarker detection.

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“…Furthermore, electrochemical reactions at the electrodes resulting in the formation of ions of PBS lead to the erosion of electrodes forming metal hydroxides. 47 Besides electrochemical reactions, the generation of tinier of bubbles at the electrodes could lead to pitting by cavitation and erosion of electrodes. Without passivation, at a DC voltage of 10 V, the electrodes were degraded within 2-3 min of operation.…”

Section: A Electrode Passivation and Transmembrane Potentialmentioning

confidence: 99%

Continuous electrical lysis of cancer cells in a microfluidic device with passivated interdigitated electrodes

et al. 2020

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Cell lysis is a critical step in genomics for the extraction of cellular components of downstream assays. Electrical lysis (EL) offers key advantages in terms of speed and non-interference. Here, we report a simple, chemical-free, and automated technique based on a microfluidic device with passivated interdigitated electrodes with DC fields for continuous EL of cancer cells. We show that the critical problems in EL, bubble formation and electrode erosion that occur at high electric fields, can be circumvented by passivating the electrodes with a thin layer (∼18 μm) of polydimethylsiloxane. We present a numerical model for the prediction of the transmembrane potential (TMP) at different coating thicknesses and voltages to verify the critical TMP criterion for EL. Our simulations showed that the passivation layer results in a uniform electric field in the electrode region and offers a TMP in the range of 5-7 V at an applied voltage of 800 V, which is well above the critical TMP (∼1 V) required for EL. Experiments revealed that lysis efficiency increases with an increase in the electric field (E) and residence time (t r ): a minimum E ∼ 10 5 V/m and t r ∼ 1.0 s are required for efficient lysis. EL of cancer cells is demonstrated and characterized using immunochemical staining and compared with chemical lysis. The lysis efficiency is found to be ∼98% at E = 4 × 10 5 V/m and t r = 0.72 s. The efficient recovery of genomic DNA via EL is demonstrated using agarose gel electrophoresis, proving the suitability of our method for integration with downstream on-chip assays.

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Electrochemical attack and corrosion of platinum electrodes in dielectrophoretic diagnostic devices

Cited by 7 publications

References 30 publications

Dielectrophoresis: Developments and applications from 2010 to 2020

Dielectrophoresis: Developments and applications from 2010 to 2020

Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021

Continuous electrical lysis of cancer cells in a microfluidic device with passivated interdigitated electrodes

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