Electrochemical Behavior of Zanamivir at Gold-Modified Boron-Doped Diamond Electrodes for an Application in Neuraminidase Sensing

Abstract

This study emphasizes the electrochemical detection of Neuraminidase (NA) based on NA inhibition by Zanamivir. The detection method was developed based on the difference of electrochemical responses of Zanamivir in the presence and the absence of NA in phosphate buffer solution (pH 5.5). Gold and gold-modified boron-doped diamond was used as the working electrode and the measurement was conducted using cyclic voltammetry method. A linear calibration curve of Zanamivir was observed in the concentration range of 5 × 10−4 mol/L (R 2 = 0.991), with the estimated limit of detection (LOD) of 1.49 × 10 −6 mol/L. The presence of NA increases the peak current of gold oxidation and reduction with linear calibration curves were monitored in the concentration range of 0-15 mU (R 2 = 0.996). An estimated LOD of 0.25 mU NA and an excellent reproducibility of the detection with an RSD of 1.18% can be achieved. Application of a real sample was successfully demonstrated for NA detection in the presence of mucin, suggested that the method is promising for pharmaceutical or medical application.

Micro-band Boron-doped Diamond Electrode in Capillary Electrophoresis for Simultaneous Detection of AMP, ADP, and ATP

“…BDD is known as an electrode that exhibits interesting electrochemical properties, such as low background current and wide potential window. These are excellent characteristics of sensor applications (Wahyuni et al, 2015;Hayat et al, 2019). The wide potential window in aqueous media has also been reported and proven to suppress the evolution of H2 in applications of CO2 reduction (Natsui et al, 2018;Jiwanti et al, 2020).…”

Electroanalysis of Pharmaceuticals on Boron‐Doped Diamond Electrodes: A Review

“…Neuraminidase (NA) is an exosialidase enzyme that is present in many pathogenic microbes and viruses. Wahyuni and coauthors characterized the electrochemical behavior of Zanamivir (a NA inhibitor), in the presence of NA and 0.33 mg mL −1 of mucin Bovine Submaxillary Glands type I‐S M3895, using CV in 0.1 mol L −1 PBS (pH 5.5) at BDD electrodes modified with gold nanoparticles, the formation of Au microarray electrode at BDD electrode provides the more sensitive responses in comparison to that of gold bulk electrode. This modification was performed in 1×10 −3 mol L −1 HAuCl 4 ⋅4H 2 O solution in 5×10 −4 mol L −1 H 2 SO 4 solution by applying 200 mV during 100 s. The calibration curve was linear in the concentration range from 5×10 −6 to 1×10 −4 mol L −1 .…”

Modification of Boron-doped Diamond Electrodes with Platinum to Increase the Stability and Sensitivity of Haemoglobin-based Acrylamide Sensors

“…(12,13) Some techniques that are generally used to modify BDD with metals have been reported, such as electrochemical deposition, photochemical reaction, and ion implantation. (10,11,(14)(15)(16) However, these methods could not achieve a suitable amount or high stability of the deposited metal on the surface BDD. (10,11,14) Moreover, the ion implantation method gives a more promising result that indicates the high stability of metal particles, but it needs a quite expensive special instrument .…”

“…(10,11,(14)(15)(16) However, these methods could not achieve a suitable amount or high stability of the deposited metal on the surface BDD. (10,11,14) Moreover, the ion implantation method gives a more promising result that indicates the high stability of metal particles, but it needs a quite expensive special instrument . (10,11) In this study, we propose to develop platinum-modified BDD (Pt-BDD) with high stability and sensitivity for application in acrylamide biosensors.…”