Electrochemical biosensing strategy for highly sensitive pesticide assay based on mercury ion-mediated DNA conformational switch coupled with signal amplification by hybridization chain reaction

Yang, Yi‐Chun; Liu, Xiaojuan; Wu, Min; Wang, Xiuzhong; Hou, Ting; Li, Feng

doi:10.1016/j.snb.2016.06.044

Cited by 23 publications

(13 citation statements)

References 45 publications

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“…In the absence of pesticide molecules, ATCh molecules are catalyzed by AChE enzyme to produce thiocholine (TCh). The thiol group (−SH) contained in TCh makes it reactive with sequestered Hg 2+ because of the reported higher affinity between −SH and Hg 2+ than that of T-T mismatch. ,, As a consequence, Hg 2+ ions are released from the hairpin structured HP, generating many unfolded HP with single-stranded structure. Since the 5′- and 3′-end of the padlock probe are complementary to the HP, it can then be ligated and circularized by using the single-stranded HP as a template and T4 DNA ligase as a tool enzyme.…”

Section: Resultsmentioning

confidence: 99%

“…Although impressive progress has been made, the key problem in these methods is that the immobilization of enzymes usually leads to the impairment of the enzyme bioactivity. To overcome this problem, our group has been devoted to developing electrochemical biosensors that need no immobilization of AChE onto the electrode surface. , In our previous work, we developed a new electrochemical biosensor based on the combination of AChE catalysis and signal amplification via hybridization chain reaction (HCR) to realize sensitive detection of pesticide residues . However, as AChE-catalyzed hydrolysis product-triggered DNA configurational change and HCR reaction occur on the solution–electrode interface, the spatial hindrance and the loss of configurational freedom usually give this heterogeneous analysis relatively lower binding efficiency than that of the homogeneous analysis.…”

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confidence: 99%

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Label-Free Homogeneous Electroanalytical Platform for Pesticide Detection Based on Acetylcholinesterase-Mediated DNA Conformational Switch Integrated with Rolling Circle Amplification

et al. 2017

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This study addresses the need for sensitive pesticide assay by reporting a new label-free and immobilization-free homogeneous electroanalytical strategy, which combines acetylcholinesterase (AChE)-catalyzed hydrolysis product-mediated DNA conformational switch and rolling circle amplification (RCA) to detect organophosphorous and carbamate pesticides in a "signal-on" mode. When target pesticides were present, AChE activity was inhibited and could not trigger the following DNA conformational change and the RCA reaction, which results in numerous methylene blue (MB) molecules in a free state, generating a strong electrochemical response. This proposed strategy was highly sensitive for omethoate detection with a detection limit as low as 2.1 μg/L and a linear range from 10 to 10 000 μg/L. Furthermore, this strategy was demonstrated to be applicable for pesticide detection in real samples. Thus, this novel label-free homogeneous electroanalytical strategy holds great promise for pesticide detection and can be further exploited for sensing applications in the environment and the food safety field.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Label-Free Homogeneous Electroanalytical Platform for Pesticide Detection Based on Acetylcholinesterase-Mediated DNA Conformational Switch Integrated with Rolling Circle Amplification

et al. 2017

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“…), [ 22,23,30–32 ] small metabolites (e.g., glutathione, ATP, glucose, pesticides, etc. ), [ 12,33–35 ] nucleic acids (e.g., miRNA‐122, miRNA‐21, miRNA‐141, the DNA of Helicobacter pylori , etc.) [ 36–39 ] , proteins (e.g., methyltransferase, prion protein, Alpha‐fetoprotein and so on), [ 40–42 ] and cells (e.g., CTC cells, HeLa cell, etc.).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensors Employing Hybridization Chain Reaction: From Structural Design to Applications

et al. 2022

Adv Materials Inter

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Electrochemical biosensors are one of the most emerging sensor technologies for many applications including disease diagnosis, environmental monitoring, and food safety, etc. They often rely on amplification strategies to achieve ultrasensitivity for the specific analytes of interest, as they feature extremely low abundance, such as ctDNA and other protein‐type cancer biomarkers. Among all the amplification strategies, hybridization chain reaction (HCR) is extremely cost‐effective, simple, enzyme‐free, and reacts under isothermal conditions, thus often employed in the electrochemical biosensors towards the goal of sensitivity enhancement. By coupling HCR and electrochemistry, it has proved to benefit a great variety of analytes, including ions, small molecules, nucleic acids, proteins, and cells, etc. Given the recent advance of HCR‐amplified electrochemical biosensors, here one aims to review the significant achievements of this platform over the past 10 years, covering their design in HCR constructs in response to the respective targets, and their challenges and opportunities.

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“…The usage of pesticides in agriculture is very critical for the increase in crop productivity . However, the increase in the amount of pesticides’ residue and their metabolites due to too much usage of carbamates and organophosphates (OPs) can cause damage to the environment and human health . The toxic effect of OPs in vertebrates was studied in detail, where OPs is also known as one of the widely used types of pesticides .…”

Section: Introductionmentioning

confidence: 99%

High Stability of Immobilized Acetylcholinesterase on Chitosan Beads

Işık

2020

ChemistrySelect

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The enzymes are unstable and have a short lifetime; this causes a decrease in the number of enzyme usage and increases the cost. In the study, the acetylcholinesterase (AChE) enzyme was covalently immobilized to the surface of chitosan via glutaraldehyde by cross-linking method, where glutaraldehyde contains functional amine group. The immobilized enzyme was characterized by scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX). The operational and storage stability of immobilized AChE was found higher than the free enzyme by the spectrophotometric method. The storage stability of the immobilized AChE was found 81% for 60 days, while the free AChE lost a high ratio of its activity in 30 days. It was observed that 93% activity was preserved for the immobilized enzyme after reusing it approximately 30 times. Moreover, kinetic parameters, the Km and Vmax, were found 0.073 mM and 0.244 EU/mL for immobilized AChE and 0.044 mM and 0.362 EU/mL for free AChE, respectively. The AChE enzyme is used in the analysis of pesticides; that is why the high stability and reusability of the enzyme as a result of immobilization proses are important in biotechnological applications.[a] Dr. M. Işık

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Electrochemical biosensing strategy for highly sensitive pesticide assay based on mercury ion-mediated DNA conformational switch coupled with signal amplification by hybridization chain reaction

Cited by 23 publications

References 45 publications

Label-Free Homogeneous Electroanalytical Platform for Pesticide Detection Based on Acetylcholinesterase-Mediated DNA Conformational Switch Integrated with Rolling Circle Amplification

Label-Free Homogeneous Electroanalytical Platform for Pesticide Detection Based on Acetylcholinesterase-Mediated DNA Conformational Switch Integrated with Rolling Circle Amplification

Electrochemical Biosensors Employing Hybridization Chain Reaction: From Structural Design to Applications

High Stability of Immobilized Acetylcholinesterase on Chitosan Beads

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