Electrochemical biosensor based on biomimetic material for myoglobin detection

Abstract

a b s t r a c tA novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVC COOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification.The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 g mL −1 , of positive slope 6.50 ± 1.48 (k mL g −1 ). The limit of detection was 2.25 g mL −1 . Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 g mL −1 . A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 A mL g −1 . MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.

Selective electrochemical sensing of human serum albumin by semi-covalent molecular imprinting

“…Until now, the semi-covalent imprinting was not used for protein templates. There are some examples of MIP fabrication where proteins were covalently immobilized on the substrate surface (Moreira et al, 2013a(Moreira et al, , 2013b(Moreira et al, , 2013cTretjakov et al, 2013). Moreover, cytochrome c was labeled with a cleavable functional monomer bearing a disulfide bond and terminal maleimide moiety capable of binding cys-17 located on the cytochrome c surface (Suga et al, 2013).…”

Protein-responsive polymers for point-of-care detection of cardiac biomarker

“…The present biosensor does not have a better analytical performance than most of the previous devices employing molecular imprinting procedures [67,68,28,25]. However, its response is among the range of concentration of clinical significance and the device set-up is by far the quicker, the simplest and the cheaper process presented so far.…”

Molecularly-imprinted polymer sensors: realising their potential

“…Production cost may be tackled by using cheaper starting materials such as silica, screen-printed electrodes, disposable plastic fibre optics, disposable biochips and paper etc (Wan et al, 2013;Moreira et al, 2013;Bakas et al, 2014;Feng et al, 2014;Ton et al, 2015;Hong et al, 2010;Wang et al 2013). Wan et al (2013) used cheaper materials as the core and then imprinted the target into shell in order to limit the production cost.…”

“…Wan et al (2013) used cheaper materials as the core and then imprinted the target into shell in order to limit the production cost. Another case is the use of inexpensive screen-printed carbon/gold electrodes to design MIP-based biosensor architectures through surface imprinting (Moreira et al, 2013), sol-gel (Bakas et al, 2014) and dual-template imprinting . In addition, other disposable materials including evanescent-wave plastic fibre optics, olefin-based microfluidics and especially paper have recently attracted much more attention as a substrate material to develop MIP-based biosensors ( Figure 9).…”