Electrochemical biosensor for assessment of the total antioxidant capacity of orange juice beverage based on the immobilizing DNA on a poly l-glutamic acid doped silver hybridized membrane

Wang, Xueliang; Jiao, Chunlei; Yu, Zhangyu

doi:10.1016/j.snb.2013.11.025

Cited by 38 publications

(12 citation statements)

References 35 publications

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“…Thus, immobilization of DNA on multi‐walled carbon nanotubes resulted in about 30 % changes of R et after Fenton reagent incubation 30. In this work, damaging effect of reactive oxygen species can be enhanced by silver ions 39 and quinone units 40, 41 of thiacalix[4]arene derivative as was reported in other investigations on oxidative DNA damage. Thus, the reaction of ascorbic acid with quinone structure can produce hydroxyl radicals even in the absence of metal ions as cations 40.…”

Section: Resultssupporting

confidence: 75%

Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

et al. 2015

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1IntroductionSince 1990, deoxyribonucleica cid (DNA) has been utilized as biorecognition element in the assembly of electrochemical biosensors.T he interest to DNAa satarget and tool of biochemical assayi sr elated to critical importance of this biomolecule in the storage and transfero f genetici nformation [1,2].T he investigation of molecular recognition with participation of DNAi sd emanded in molecular biology,t oxicology,m edicine,p harmacy and many other areas of research activity considering human health problems.I na ddition to detection of DNAÀana-lyte interactionsi ncluding genetic polymorphism,h ybridization events etc.,t he DNAd amage,w hich potentially results in many diseases like cancer, is recentlyi nt he focus of many research efforts [3,4].D ifferentt ypes of the DNAd amage induced by reactiveo xygen species [5], UV exposure[ 6],a nd mutagenic species [7][8][9] were successfully detectedbyb iosensor approaches.Fast screening of DNAd amage is vital for understanding the mechanisms and establishing necessary preventive measures.T he electrophoresis mainly applied for detection of the DNAs trand breaks [10] is rather sensitiveb ut cannot be used in field conditions. Electrochemical DNAbased biosensors are portable devices integrating DNAa s biorecognitionelementand an electrode as physicochemical transducer [ 11].T he progress in detection of the DNAd amage by biosensors is caused by undisputed advantages that involves imple design and measurement protocol, sufficient sensitivity,c ompatibility with conventional electrochemical equipment and analogy to real redox-processes that take place in living cells under oxidative stress.I ts hould be also mentioned that many of the protocols of the DNAd amage detection are applied for the estimationo fp rotecting effecte xerted by antioxidants.A ssessment of the antioxidant activity is required in agriculture and food quality analysis [6,[12][13][14].Electrochemical detection of the oxidativeD NA damage is mainly based on recording signals referred to selected nucleic basesa nd productso ft heir transformation [15][16][17][18]. Thus,t he accessibility of guaninef or direct oxidation on the electrode increased withD NA helix distortion. Fort he samer eason, increased peak of guanine oxidation is often followed by appearance of the peak of biomarker of its oxidation, 8-oxo-guanine [19].G uanine oxidation can be promoted by redox mediators either directly or via formation of oxygen radical species.T his acceleratest he DNAo xidationi nt he presence of dissolved oxygen as as ource for catalytic formationo fs uperoxide anionr adical. Such reactionsw ere describedf or bipyridyl [20][21][22] and phenanthroline [23][24][25] complexes of Ru, Co and some other transient metals.C hanges in the DNA structurec ausedb yi ts damage influence the intercalation of redox active species,e .g.,M ethyleneb lue thatc hanges Abstract:N ovele lectrochemical DNA-sensor based on glassy carbon electrode( GCE) modified with Ag nanoparticles,N eutral red covalently attached to its surfa...

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Section: Resultssupporting

confidence: 75%

Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

et al. 2015

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“…Recently, different biosensors have been reported in order to measure the antioxidant capacity of wine [ 29 ], fruit [ 30 ], infusions [ 31 ] and medicinal plant extracts [ 32 ]. The present research work shows that after mushroom tyrosinase is immobilized through the entrapment and cross-linking methods, under three different experimental conditions, the apparent Michaelis-Menten constant for all of them is used as a parameter to establish the system's kinetic behavior, the reaction product's formation rate and other kinetic, physicochemical and analytic parameters.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors

Rodríguez-Sevilla

Ramírez-Silva

Romero-Romo

et al. 2014

Sensors

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The working area of a screen-printed electrode, SPE, was modified with the enzyme tyrosinase (Tyr) using different immobilization methods, namely entrapment with water-soluble polyvinyl alcohol (PVA), cross-linking using glutaraldehyde (GA), and cross-linking using GA and human serum albumin (HSA); the resulting electrodes were termed SPE/Tyr/PVA, SPE/Tyr/GA and SPE/Tyr/HSA/GA, respectively. These biosensors were characterized by means of amperometry and EIS techniques. From amperometric evaluations, the apparent Michaelis-Menten constant, Km′, of each biosensor was evaluated while the respective charge transfer resistance, Rct, was assessed from impedance measurements. It was found that the SPE/Tyr/GA had the smallest Km′ (57 ± 7) μM and Rct values. This electrode also displayed both the lowest detection and quantification limits for catechol quantification. Using the SPE/Tyr/GA, the Trolox Equivalent Antioxidant Capacity (TEAC) was determined from infusions prepared with “mirto” (Salvia microphylla), “hHierba dulce” (Lippia dulcis) and “salve real” (Lippia alba), medicinal plants commonly used in Mexico.

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“…Recently, also electrochemical methods have greatly contributed to measure of TAC based on biosensors [28,29,30,31] and sensors [32,33]. They offer sensitivity, inexpensive instrumentation, fast response, small volumes [34,35,36,37].…”

Section: Introductionmentioning

confidence: 99%

Metal Oxide Nanoparticle Based Electrochemical Sensor for Total Antioxidant Capacity (TAC) Detection in Wine Samples

et al. 2018

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A single-use electrochemical screen-printed electrode is reported based on biomimetic properties of nanoceria particles (CeNPs). The developed tool showed an easy approach compared to the classical spectrophotometric methods reported in literature in terms of ease of use, cost, portability, and unnecessary secondary reagents. The sensor allowed the detection of the total antioxidant capacity (TAC) in wine samples. The sensor has been optimized and characterized electrochemically and then tested with antioxidant compounds occurred in wine samples. The electrochemical CeNPs modified sensor has been used for detection of TAC in white and red commercial wines and the data compared to the 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS)-based spectrophotometric method. Finally, the obtained results have demonstrated that the proposed sensor was suitable for the simple and quick evaluation of TAC in beverage samples.

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Electrochemical biosensor for assessment of the total antioxidant capacity of orange juice beverage based on the immobilizing DNA on a poly l-glutamic acid doped silver hybridized membrane

Cited by 38 publications

References 35 publications

Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors

Metal Oxide Nanoparticle Based Electrochemical Sensor for Total Antioxidant Capacity (TAC) Detection in Wine Samples

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