Electrochemical biosensor for detection of MON89788 gene fragments with spiny trisoctahedron gold nanocrystal and target DNA recycling amplification

Peng, Yuanfeng; Ruiyi, Li; Yu, Minyi; Yi, Xiaowen; Zhu, Haiyan; Li, Zaijun; Yang, Yongqiang

doi:10.1007/s00604-020-04467-5

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…Previous studies have also confirmed the regulation. 41,42 CV was used to scan the surface of the electrode at different scan rates to determine the dynamics on the modified gold electrode surface. In Figure 3c, the peak current of the redox process gradually increases with the increase of scan rates from 30 to 300 mV/s.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Sensor for Directional Recognition and Measurement of Antibiotic Resistance Genes in Water

Wan

et al. 2021

Anal. Chem.

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The establishment of rapid targeted identification and analysis of antibiotic resistance genes (ARGs) is very important. In this study, an electrochemical sensor, which can detect ARGs was obtained by modifying the sulfhydryl single-stranded DNA probe onto the thin-film gold electrode through self-assembly. The sensor can perform a hybridization reaction with a target sequence to obtain an electrochemical impedance spectroscopy signal. The results showed that when the concentration of the probe used to modify thin-film gold electrodes during preparation was 1 μM, the hybridization time was 1 h, and the hybridization temperature was 35 °C, the self-assembled sensor showed good detection performance for the ARGs encoding β-lactam hydrolase. The measurement ARG concentration linear range is 6.3–900.0 ng/mL, and the R 2 is 0.9992. The sensor shows good specific recognition ability for single-base, double-base, and three-base mismatch DNA. In addition, after 30 days of storage at 4 °C, the accurate identification and analysis of ARGs can still be maintained.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Sensor for Directional Recognition and Measurement of Antibiotic Resistance Genes in Water

Wan

et al. 2021

Anal. Chem.

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“…Trisoctahedral gold seeds (TO–Au) were prepared using the reported method. 19 The formed TO–Au were endowed with an asymmetrical structural evolution induced by the ECGECE hexapeptide. Typically, HAuCl 4 solution was mixed with KBr solution and KI solution to form growth solution II comprising 0.4 mM HAuCl 4 , 15 mM CTAC, 10 μM KBr and 1 μM KI.…”

Section: Methodsmentioning

confidence: 99%

“…The resultant chiral gold nanocrystals displays high stereoselectivity and catalytic activity. 19 However, the gold nanocrystals prepared using the seed growth method do not have a three-dimensional structure. This causes the partial blockage of channels for electron transfer between the electrode and the electrolyte, low atom utilization and instability.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of coral-like gold nanocrystals with the glutamic acid–cysteine–glycine–glutamic acid–cysteine–glycine hexapeptide for the electrochemical detection of α-amanitin in urine

et al. 2023

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Electrochemical biosensor for detection of MON89788 gene fragments with spiny trisoctahedron gold nanocrystal and target DNA recycling amplification

Cited by 2 publications

References 41 publications

Electrochemical Sensor for Directional Recognition and Measurement of Antibiotic Resistance Genes in Water

Electrochemical Sensor for Directional Recognition and Measurement of Antibiotic Resistance Genes in Water

Synthesis of coral-like gold nanocrystals with the glutamic acid–cysteine–glycine–glutamic acid–cysteine–glycine hexapeptide for the electrochemical detection of α-amanitin in urine

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