Electrochemical biosensor for the detection of a sequence of the TP53 gene using a methylene blue labelled DNA probe

Otero, Fernando; Shortall, Kim; Salaj-Kośla, Urszula; Tofail, Syed A. M.; Magner, Edmond

doi:10.1016/j.electacta.2021.138642

Cited by 18 publications

(7 citation statements)

References 39 publications

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“…The detection limit of the electrochemical DNA sensor was up to 10 nM due to the hairpin structure and signal amplification of MB. The obtained detection limit for TP53 is significantly better than that of the field-effect transistor sensor (100 nM) [ 45 ], and is comparable to that of the electrochemical biosensor developed by Otero et al [ 37 ]. With an increase in cDNA concentration, the binding efficiency of the double-stranded was improved, and a larger amount of MB was far away from the electrode surface, resulting in a significant decrease in peak current.…”

Section: Resultsmentioning

confidence: 68%

“…The DNA surface coverage (

, pmol/cm 2 ) was calculated using Equation (1).

where Q (C), n , F , and A eff represent the reduced charge of an electrochemical indicator (MB) in cyclic voltammetry obtained for the ssDNA-Au electrodes, the quantity of electrons involved in the reaction (2e − ), the Faraday constant (96,485 × 10 −12 C/pmol), and the effective area of the Au disk electrode, respectively [ 36 , 37 ]. The DNA surface coverage was determined by integrating the second scan’s reduction peak of cyclic voltammograms.…”

Section: Methodsmentioning

confidence: 99%

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Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot

Sun

Niu

et al. 2022

Biosensors

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Rapid and sensitive detection of cancer biomarkers is crucial for cancer screening, early detection, and improving patient survival rate. The present study proposes an electrochemical gene-sensor capable of detecting tumor related TP53 gene mutation hotspots by self-assembly of sulfhydryl ended hairpin DNA probes tagged with methylene blue (MB) onto a gold electrode. By performing a hybridization reaction with the target DNA sequence, the gene-sensor can rearrange the probe’s structure, resulting in significant electrochemical signal differences by differential pulse voltammetry. When the DNA biosensor is hybridized with 1 μM target DNA, the peak current response signal can decrease more than 60%, displaying high sensitivity and specificity for the TP53 gene. The biosensor achieved rapid and sensitive detection of the TP53 gene with a detection limit of 10 nmol L−1, and showed good specific recognition ability for single nucleotide polymorphism (SNP) and base sequence mismatches in the TP53 gene affecting residue 248 of the P53 protein. Moreover, the biosensor demonstrated good reproducibility, repeatability, operational stability, and anti-interference ability for target DNA molecule in the complex system of 50% fetal bovine serum. The proposed biosensor provides a powerful tool for the sensitive and specific detection of TP53 gene mutation hotspot sequences and could be used in clinical samples for early diagnosis and detection of cancer.

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Section: Resultsmentioning

confidence: 68%

“…The DNA surface coverage (

, pmol/cm 2 ) was calculated using Equation (1).

Section: Methodsmentioning

confidence: 99%

Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot

Sun

Niu

et al. 2022

Biosensors

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“…15 However, the requirement for a labelling step to transduce the hybridization event, electrode fouling, and the difficulty in distinguishing the electrochemical signal produced by targets with a low concentration from the background signal are the main limitations of an electrochemical genosensor, that can result in inaccurate results. [16][17][18] Therefore, efforts have been made to use a gene-specic bioreceptor for the fabrication of a specic biosensing platform. In this context, the mA gene of E. coli shows enormous potential for the development of a genosensor for NS detection.…”

Section: Introductionmentioning

confidence: 99%

Designing of a unique bioreceptor and fabrication of an efficient genosensing platform for neonatal sepsis detection

et al. 2023

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“…Most of these products are expensive and difficult to synthesize. As a result, redox‐active substances such as methylene blue [ 32 ] and ferrocene [ 33 ] are widely used that can directly obtain signals and are simple, inexpensive, and reversible. However, methylene blue and ferrocene only produce one electron, and two electrons transfer during a redox process, leading to low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…Most of these products are expensive and difficult to synthesize. As a result, redox-active substances such as methylene blue [32] and ferrocene [33] are widely used that can directly obtain signals and are simple, inexpensive, and reversible.…”

Section: Introductionmentioning

confidence: 99%

A sensitive electrochemical DNA sensor based on reduced graphene oxide modified electrode

Shu

et al. 2022

J Chinese Chemical Soc

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In this work, an electrochemical sensing platform, analogous to a DNA walker, was explored with exonuclease III (EXO III) as a driving force, tetraferrocene as a signal marker, and reduced graphene oxide (RGO) electrode as twodimensional (2D) orbit to realize ultrasensitive detection of target DNA. We designed a single hairpin DNA probe with 3 0 -end modified tetraferrocene, immobilized on the electrode surface by π-π stacking between the 5 0 terminus and reduced graphene oxide. In addition, EXO III digested the double-stranded DNA with a blunt 3 0 terminus formed by the target DNA and the probe DNA, thus degrading the 3 0 end of probe DNA, and resulting in the away movement of tetraferrocene from the electrode surface, decreasing the electrochemical signal. The proposed electrochemical sensor was very simple and stable with acceptable reproducibility, excellent DNA detection performance, and a detection limit as low as 0.3 pM.

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Electrochemical biosensor for the detection of a sequence of the TP53 gene using a methylene blue labelled DNA probe

Cited by 18 publications

References 39 publications

Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot

Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot

Designing of a unique bioreceptor and fabrication of an efficient genosensing platform for neonatal sepsis detection

A sensitive electrochemical DNA sensor based on reduced graphene oxide modified electrode

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