Electrochemical Biosensors Based on Enzymatic Reactor of Silver Solid Amalgam Powder for Measurements in Flow Systems

Josypčuk, Oksana; Barek, Jiřı́; Josypčuk, Bohdan

doi:10.1002/elan.201400224

Cited by 14 publications

(29 citation statements)

References 33 publications

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“…The mechanism of oxygen reduction on various electrodes is different and it usually proceeds via two well‐separated two‐electron steps on mercury and amalgam electrodes . The first step is due to reduction of oxygen to hydrogen peroxide and the second step corresponds to reduction of hydrogen peroxide to water.…”

Section: Resultsmentioning

confidence: 99%

“…Necessary optimization experiments were performed to determine the properties and possibilities of biosensors based on the reactor with enzyme modified silver amalgam powder . The optimal composition of the used mobile phase was [0.1 molL −1 AcB; 1 mmolL −1 Na 2 EDTA; pH 6.5].…”

Section: Resultsmentioning

confidence: 99%

“…The injection loops and all connections in the flow system were done by PTFE tubing with the inner diameter ( ID ) 0.50 mm and the outer diameter ( OD ) 1.59 mm. Scheme of used FIA‐ED is shown and described in details in our previous work .…”

Section: Methodsmentioning

confidence: 99%

“…Two main constructions of the electrochemical biosensors were used: i) biorecognition element (enzyme, avidin, oligonucleotide, etc.) is anchored at a working electrode surface either directly, or through an additional thin layer, for example of chitosan hydrogel ; ii) biorecognition element is adsorbed or covalently bound to a surface that is spatially separated from the working electrode . In the last case we talk about the use of some type of a reactor, and hence the enzymatic biosensor consists of two separate parts: the enzymatic reaction takes place in the reactor, and the detector (working electrode) then registers either some product of enzymatic reaction or, e.g., decrease/increase of oxygen concentration in the solution.…”

Section: Introductionmentioning

confidence: 99%

“…The large surface of the enzymatic reactor (ER) can be provided by various porous or powdered materials and the sensitivity of the enzyme biosensor depends on the area covered by enzyme. Different types of silicate materials were widely used as a surface to anchor the biorecognition elements.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

2016

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Two enzymatic biosensors with amalgam powder reactors and twelve enzymatic biosensors with various silica powder reactors were fabricated and tested in this work. The enzymatic reactors based on silver amalgam powder provide high sensitivity and they are convenient for faster flow rates. Experiments with six silica materials showed that mesoporous silica SBA‐15 was the best one in terms of covering by enzymes, sensitivity and lifetime of biosensors. The current response of the SBA15‐glucose oxidase (GOx) sensor started to decrease only after 200 day testing and after 406 days the peak current was still 35.9 % of the initial value. The used amalgam tubular detector in a flow system allowed working at highly negative potentials, which significantly increased the sensitivity of determinations. Statistical results of parallel measurement of model solutions with the fabricated biosensors show their high accuracy (RSD=0.28–1.81 %) and sensitivity (6.2–14.3 µmol L−1). The proposed SBA15‐GOx biosensor was successfully used for determination of glucose in commercial honey.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

2016

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Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine

2015

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1IntroductionAmperometrical biosensors based on enzymes were introduced by Clark and Lyons in 1962. Since this timeu pt o the present, biosensors have faced am assive growth in usage for various applications [1].E lectrochemical enzyme biosensors are one of the manyt ypes of biosensors which are constitutedb yi mmobilized enzyme as ab iological recognition element and an electrochemical transducer which converts the analytical signal produced as ar esult of the biochemical and electrochemical interactionsi nto measurable electricals ignal [2].A lthough, using enzymes has somed isadvantages such as high price of some enzymes,r elatively short operation lifetime, or negative effecto fp resent of inhibitors,t he immobilized enzymes provide one very important advantage -depending on enzymes specific, they have ability to recognize monitored analyte or group of analytes (substrates) with high selectivity and catalyze their transformation. These unique properties make the enzymes powerfult ools to developa nalytical devices [2][3][4][5].Besides the enzyme,t he main part of electrochemical biosensor is an electrochemical transducer -a ne lectrode (detector). Nowadays,l iterature quotes various kinds of electrode materials such gold,b oron doped diamond or different types of carbon [6][7][8][9][10].N evertheless, in most cases they are more suitable and used in positive region of potentials.T he offer of electrodes suitable for measurement at negativep otential is much smaller. Probably the best material proves to be mercury due to high hydrogen overvoltage.U nfortunately,aliquid state of mercury causes problems with mechanical stability of mercury drop what meanst hat prepared biosensor will be also mechanically unstable.I ts hows especially when such biosensor is used in flow system. Ag ood solution is substitution of mercury with silver solid amalgam( AgSA).T he electrode of silver solida malgam( AgSAE)c ould be simply prepared of the necessary shape and size,a nd depending on the preparation technique and on the ratio of metal to mercury, the final electrode material could be solid [11][12][13][14] [20].A tt he same time,A gSAEs allows to work in aqueouss olutions up to À2V .T here are manyp ublished works and reviewsw hich are demonstrating the use of AgSAEs in both batch and flow systems[ 21-24] for determinations of variety of reducing compounds in environmental and pharmaceuticala nalysis.I no ur previous works,w ed escribed preparation and successfula pplication of two types of enzymatic biosensors based on AgSA. Them ain concept of these biosensors consists in use of two parts -aflow reactor [ 18,25] where the enzymatic reactiont akes place,a nd at ubularf low detector [23] where increasing or decreasing the concentration for somec omponent of this enzymatic reaction is measured. Both the reactor and the detector are based on AgSA but of different types.W hile tubular detectori sm adef rom compacts mootha malgam, the consistence of AgSA of the reactor is porous or powdered. It demonstrates wide possibilities of...

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Applying Mesoporous Silica SBA‐15 in Electrochemical Detection of DNA Hybridization

et al. 2016

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Mesoporous silica SBA‐15 powder was used as the separation surface for hybridization purposes and it has proved appropriate and effective. By help of a simple procedure any oligodeoxynucleotide (ODN) modified by NH2‐group can be covalently bound at the surface of SiO2 particles, what significantly extends the capabilities of the experimenter. Use of cathodic stripping voltammetry for electrochemical detection of ODN/DNA hybridization ensures high sensitivity of the analyses. Only ordinary laboratory equipment is needed for the implementation of these experiments. Due to the very small consumption of SBA‐15, commonly sold quantity (5 g) may be sufficient for many years of work.

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Electrochemical Biosensors Based on Enzymatic Reactor of Silver Solid Amalgam Powder for Measurements in Flow Systems

Cited by 14 publications

References 33 publications

Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

Electrochemical Biosensors Based on Enzymatic Reactors Filled by Various Types of Silica and Amalgam Powders for Measurements in Flow Systems

Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine

Applying Mesoporous Silica SBA‐15 in Electrochemical Detection of DNA Hybridization

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