Electrochemical Biosensors Based on S-Layer Proteins

Damiati, Samar; Schuster, Bernhard

doi:10.3390/s20061721

Cited by 40 publications

(19 citation statements)

References 153 publications

(234 reference statements)

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“…Apparently, some are very resistant to disintegration into their constituent glycoprotein subunits, (e.g., by boiling in SDS) indicating the possible presence of covalent inter-subunit bonds [26,32,111]. SUMs made of S-layers from extremophiles should enable to produce membranes with very little leakage and excellent biocompatibility as required for blood purification and fractionation (e.g., aphaeresis), implants, and biosensors development [97,98].…”

Section: Discussionmentioning

confidence: 99%

“…SUMS were also used as supporting matrix for biologically active components to produce amperometric biosensors [82,83,87,97,98]. In Figure 6, a generalized scheme of the construction of an SUM-based biosensor is shown.…”

Section: S-layers As Matrix For the Immobilization Of Functional Macrmentioning

confidence: 99%

“…A thin metal coating was generated either by sputtering or pulse-laser deposition in order to achieve a better electron transfer between the immobilized enzymes and the working electrode. The glucose and sucrose sensor showed a linear range of up to 12 mM and 30 mM and a stability of 48 h and 36 h, respectively [82,83,98]. The fields of application of the above described SUM-based biosensors are blood analysis, microbiology, and food technology [87].…”

Section: S-layers As Matrix For the Immobilization Of Functional Macrmentioning

confidence: 99%

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S-Layer Ultrafiltration Membranes

Schuster

Sleytr

2021

Membranes

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Monomolecular arrays of protein subunits forming surface layers (S-layers) are the most common outermost cell envelope components of prokaryotic organisms (bacteria and archaea). Since S-layers are periodic structures, they exhibit identical physicochemical properties for each constituent molecular unit down to the sub-nanometer level. Pores passing through S-layers show identical size and morphology and are in the range of ultrafiltration membranes. The functional groups on the surface and in the pores of the S-layer protein lattice are accessible for chemical modifications and for binding functional molecules in very precise fashion. S-layer ultrafiltration membranes (SUMs) can be produced by depositing S-layer fragments as a coherent (multi)layer on microfiltration membranes. After inter- and intramolecular crosslinking of the composite structure, the chemical and thermal resistance of these membranes was shown to be comparable to polyamide membranes. Chemical modification and/or specific binding of differently sized molecules allow the tuning of the surface properties and molecular sieving characteristics of SUMs. SUMs can be utilized as matrices for the controlled immobilization of functional biomolecules (e.g., ligands, enzymes, antibodies, and antigens) as required for many applications (e.g., biosensors, diagnostics, enzyme- and affinity-membranes). Finally, SUM represent unique supporting structures for stabilizing functional lipid membranes at meso- and macroscopic scale.

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Section: Discussionmentioning

confidence: 99%

Section: S-layers As Matrix For the Immobilization Of Functional Macrmentioning

confidence: 99%

Section: S-layers As Matrix For the Immobilization Of Functional Macrmentioning

confidence: 99%

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S-Layer Ultrafiltration Membranes

Schuster

Sleytr

2021

Membranes

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“…By definition, these are self-sufficient integrated devices that provide qualitative and semi-quantitative analytical data through the use of a biological recognition element that is coupled to a transduction element. The sole purpose of these analytical devices is to rapidly provide accurate and reliable information about an analyte of interest in real time [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Biosensors: Design, Development and Applications

Tetyana¹,

Shumbula²,

Njengele-Tetyana³

2021

Nanopores

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The ability to detect even the slightest physiological change in the human body with high sensitivity and accurately monitor processes that impact human nature and their surroundings has led to an immense improvement in the quality of life. Biosensors continue to play a critical role across a myriad of fields including biomedical diagnosis, monitoring of treatment and disease progression, drug discovery, food control and environmental monitoring. These novel analytical tools are small devices that use a biological recognition system to investigate or detect molecules. This chapter covers the design and development of biosensors, beginning with a brief historical overview. The working principle and important characteristics or attributes of biosensors will also be addressed. Furthermore, the basic types of biosensors and the general applications of these biosensors in various fields will be discussed.

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“…Electrochemical analysis is one of the most widely used techniques in clinical applications due to its speed, low cost, design flexibility, and high sensitivity and selectivity (4)(5)(6)(7). Moreover, electrochemical-based biosensors can be exploited either to understand the electrochemical behavior of biomolecules or as effective tools to detect different analytes in clinical, environmental, agricultural, pharmaceutical and food samples (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity Comparison of Macro- and Micro-Electrochemical Biosensors for Human Chorionic Gonadotropin (hCG) Biomarker Detection

et al. 2019

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Selectivity and sensitivity are important figures of merit in the design and optimization of electrochemical biosensors. The efficiency of the fabricated immunosensing surface can easily be influenced by several factors, such as detection limit, non-specific binding, and type of sensing platform. Here, we demonstrate the effect of macro-and micro-sized planner working electrodes (4 mm and 400 µm diameter, respectively) on the electrochemical behavior and the performance of the developed biosensor to detect human chorionic gonadotropin (hCG), please say why hCG here briefly. The fabricated screen-printed sensor was constructed by modifying the carbon macro-and micro-electrodes with a linker, 1-pyrenebutyric acid-Nhydroxysuccinimide ester (PANHS) and immobilization of anti-hCG antibodies to detect specifically the hCG protein. The characterization of the developed electrodes was performed by cyclic voltammetry (CV) and square wave voltammetry (SWV). Each immunesensing system has its unique electrochemical behavior which might be attributed to arrangement of particles on the surface. However, the smaller surface area of the micro-electrode is found to show higher sensitivity (1 pg/mL) compared to the macro-electrode sensor with a lower detection limit of 100 pg/mL. The proposed assay represents a promising approach that is highly effective for specific detection of an analyte and can be exploited to target biomarkers for a variety of point-of-care diagnostic applications.

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Electrochemical Biosensors Based on S-Layer Proteins

Cited by 40 publications

References 153 publications

S-Layer Ultrafiltration Membranes

S-Layer Ultrafiltration Membranes

Biosensors: Design, Development and Applications

Sensitivity Comparison of Macro- and Micro-Electrochemical Biosensors for Human Chorionic Gonadotropin (hCG) Biomarker Detection

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