Electrochemical Biosensors for Antioxidants

García-Guzmán, Juan José; López-Iglesias, David; Marin, Mariana; Lete, Cecilia; Lupu, Stelian; Palacios-Santander, José María; Aguilera, Laura Cubillana

doi:10.1016/b978-0-12-815743-5.00004-4

Cited by 6 publications

(5 citation statements)

References 169 publications

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“…The electrochemical techniques mostly used, generally for polyphenols and especially for RA analysis, were amperometry for the quantitative determination and voltammetry (CV, DPV, SWV) for both the investigation of the electrochemical behavior of the analytes and their quantification. DPV and SWV may offer better selectivity [62] (e.g., the DPV simultaneous determination of RA and protocatechuic acid (PCA) at a GCE/PoPD/Pt [13]); however, as most often the analysis of polyphenols in real samples are not focused on the assessment of a given analyte, but on the evaluation of a group of compounds with similar properties (e.g., polyphenols), chronoamperometry is employed due to its enhanced sensitivity [62].…”

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

“…Voltammetric/amperometric enzymatic biosensors have been widely applied to the polyphenolic antioxidants analysis due to several advantages offered, such as high selectivity, enhanced signals due to enzyme-catalyzed electrode reactions, and thus higher sensitivity, and the easy immobilization of the enzyme on the transducer. On the other hand, biosensors have short response times and they can be miniaturized and used in online analysis [62].…”

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

“…Therefore, to protect the modified electrode from fouling, numerous enzymatic biosensors use Nafion coatings [55]. Conducting polymers were also used to improve the immobilization of the enzyme onto the electrode surface enhancing the robustness of the biosensor [62].…”

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

“…Laccases are multicopper oxidases, also named "blue" copper oxidases, widely distributed in the nature (from bacterial species to fungi and plants [67]), that catalyze the oxidation of mono-, di-, poly-phenols (including RA), amino-phenols, methoxy-phenols, aromatic amines and ascorbate, through the four-electron reduction of molecular oxygen to water [16,66,68]. Similar to laccase, tyrosinase catalyzes, in the presence of oxygen, the oxidation of the o-phenols to the corresponding o-quinone [62].…”

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

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Electrochemical Methods and (Bio) Sensors for Rosmarinic Acid Investigation

et al. 2020

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Rosmarinic acid (RA) is an important bioactive phenolic acid with significant biochemical activities, including the antioxidant one. It is widely found in plants of the families Lamiaceae and Boraginaceae and has many uses in the food, pharmaceutical and cosmetics industries. RA is an electroactive species owing to the presence of the two catechol groups in its structure. Due to their inherent characteristics, such as sensitivity, selectivity, ease of operation and not too high costs, electrochemical methods of analysis are interesting tools for the assessment of redox-active compounds. Moreover, there is a good correlation between the redox potential of the analyte and its capability to donate electrons and, consequently, its antioxidant activity. Therefore, this paper presents a detailed overview of the electrochemical (bio)sensors and methods, in both stationary and dynamic systems, applied for RA investigation under different aspects. These comprise its antioxidant activity, its interaction with biological important molecules and the quantification of RA or total polyphenolic content in different samples.

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Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

Section: Electrochemical Quantitative Analysis Of Rosmarinic Acidmentioning

confidence: 99%

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Electrochemical Methods and (Bio) Sensors for Rosmarinic Acid Investigation

et al. 2020

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“…The advantages of chemically modified electrode application are demonstrated. Electrochemical enzymebased biosensors for the evaluation of the total phenolic content in medicinal plant extracts have recently been presented and discussed in detail in [45][46][47] and will not be considered in the current review.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization of the Antioxidant Properties of Medicinal Plants and Products: A Review

Ziyatdinova

Kalmykova

2023

Molecules

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Medicinal plants are an important source of bioactive compounds with a wide spectrum of practically useful properties. Various types of antioxidants synthesized in plants are the reasons for their application in medicine, phytotherapy, and aromatherapy. Therefore, reliable, simple, cost-effective, eco-friendly, and rapid methods for the evaluation of antioxidant properties of medicinal plants and products on their basis are required. Electrochemical methods based on electron transfer reactions are promising tools to solve this problem. Total antioxidant parameters and individual antioxidant quantification can be achieved using suitable electrochemical techniques. The analytical capabilities of constant-current coulometry, potentiometry, various types of voltammetry, and chrono methods in the evaluation of total antioxidant parameters of medicinal plants and plant-derived products are presented. The advantages and limitations of methods in comparison to each other and traditional spectroscopic methods are discussed. The possibility to use electrochemical detection of the antioxidants via reactions with oxidants or radicals (N- and O-centered) in solution, with stable radicals immobilized on the electrode surface, via oxidation of antioxidants on a suitable electrode, allows the study of various mechanisms of antioxidant actions occurring in living systems. Attention is also paid to the individual or simultaneous electrochemical determination of antioxidants in medicinal plants using chemically modified electrodes.

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