Electrochemical biosensors in exosome analysis; a short journey to the present and future trends in early-stage evaluation of cancers

Dezhakam, Ehsan; Khalilzadeh, Balal; Mahdipour, Mahdi; Işıldak, İbrahim; Yousefi, Hadi; Ahmadi, Mahdi; Naseri, Alireza; Rahbarghazi‬, Reza

doi:10.1016/j.bios.2022.114980

Cited by 37 publications

(13 citation statements)

References 81 publications

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“…As mentioned above, nano‐sized EVs like exosomes, microvesicles, and apoptotic bodies are actively involved in paracrine cell‐to‐cell communication (Dezhakam et al, 2022). This transport system harbors board signaling molecules metabolites, proteins, and nucleic acids from donor cells to the recipient counterparts (Amari & Germain, 2021).…”

Section: Mitochondrial Transfer and Angiogenesismentioning

confidence: 99%

Angiogenic activity of mitochondria; beyond the sole bioenergetic organelle

Sadeghsoltani,

Hassanpour,

Safari

et al. 2024

Journal Cellular Physiology

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Angiogenesis is a complex process that involves the expansion of the pre‐existing vascular plexus to enhance oxygen and nutrient delivery and is stimulated by various factors, including hypoxia. Since the process of angiogenesis requires a lot of energy, mitochondria play an important role in regulating and promoting this phenomenon. Besides their roles as an oxidative metabolism base, mitochondria are potential bioenergetics organelles to maintain cellular homeostasis via sensing alteration in oxygen levels. Under hypoxic conditions, mitochondria can regulate angiogenesis through different factors. It has been indicated that unidirectional and bidirectional exchange of mitochondria or their related byproducts between the cells is orchestrated via different intercellular mechanisms such as tunneling nanotubes, extracellular vesicles, and gap junctions to maintain the cell homeostasis. Even though, the transfer of mitochondria is one possible mechanism by which cells can promote and regulate the process of angiogenesis under reperfusion/ischemia injury. Despite the existence of a close relationship between mitochondrial donation and angiogenic response in different cell types, the precise molecular mechanisms associated with this phenomenon remain unclear. Here, we aimed to highlight the possible role of mitochondria concerning angiogenesis, especially the role of mitochondrial transport and the possible relation of this transfer with autophagy, the housekeeping phenomenon of cells, and angiogenesis.

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Section: Mitochondrial Transfer and Angiogenesismentioning

confidence: 99%

Angiogenic activity of mitochondria; beyond the sole bioenergetic organelle

Sadeghsoltani,

Hassanpour,

Safari

et al. 2024

Journal Cellular Physiology

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“…Therefore, after the injection of HP [1-13C] pyruvate, rapid 13C MR was used to measure the metabolic conversion of this aggressive cancer and its response to treatment, with direct biological significance (Fantin et al, 2006;Keshari et al, 2013a;Keshari et al, 2013b;Wilson and Kurhanewicz, 2014). Using this approach, Qi et al (2022) utilized 18F-FDG-PET and HP [1-13C]pyruvate MRSI with chemical exchange saturation transfer (CEST) MRI measures to investigate the effect of glucose infusion on intracellular pH and its relationship to the tumor.…”

Section: Molecular Imaging Technology Combined With Glycolysis Produc...mentioning

confidence: 99%

“…FDG is a radiolabeled glucose analog that is transported into tumor cells and phosphorylated by hexokinase ( Dezhakam et al, 2022 ). Therefore, FDG-PET can indicate the need for glucose by cells or tumors and provide some information about the tumor’s processing of glucose into usable components ( Cossu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Imaging glucose metabolism to reveal tumor progression

Meng

Sun²,

Zhang³

et al. 2023

Front. Physiol.

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Purpose: To analyze and review the progress of glucose metabolism-based molecular imaging in detecting tumors to guide clinicians for new management strategies.Summary: When metabolic abnormalities occur, termed the Warburg effect, it simultaneously enables excessive cell proliferation and inhibits cell apoptosis. Molecular imaging technology combines molecular biology and cell probe technology to visualize, characterize, and quantify processes at cellular and subcellular levels in vivo. Modern instruments, including molecular biochemistry, data processing, nanotechnology, and image processing, use molecular probes to perform real-time, non-invasive imaging of molecular and cellular events in living organisms.Conclusion: Molecular imaging is a non-invasive method for live detection, dynamic observation, and quantitative assessment of tumor glucose metabolism. It enables in-depth examination of the connection between the tumor microenvironment and tumor growth, providing a reliable assessment technique for scientific and clinical research. This new technique will facilitate the translation of fundamental research into clinical practice.

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“…Biosensors employ various transduction principles, including electrical [16][17][18] , optical 8,19,20 , and acoustic methods [21][22][23] . Among these, acoustic gravimetric biosensors stand out for their simplicity, robustness, and low cost, making them attractive for a wide range of applications, particularly in point-of-care (POC) settings 24,25 .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic QCM enables ultrahigh Q-factor: a new paradigm for in-liquid gravimetric sensing

Zauscher,

ZHAO,

Parlak

et al. 2024

Preprint

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Acoustic gravimetric biosensors attract attention due to their simplicity, robustness, and low cost. However, a prevailing challenge in these sensors is dissipation which manifests in a low quality factor (Q-factor), which limits their sensitivity and accuracy. To mitigate dissipation of acoustic sensors in liquid environments we introduce an innovative approach in which we combine microfluidic channels with gravimetric sensors. To implement this novel paradigm we chose the quartz crystal microbalance (QCM) as our model system, owing to its wide applicability in biosensing and the relevance of its operating principles to other types of acoustic sensors. We postulate that the crucial determinant for enhancing performance lies in the ratio between the wavelength of the pressure wave generated by the oscillating channel side walls (driven by the QCM), and the width of the microfluidic channels. Our hypothesis is supported by finite element analysis (FEA) and dimensional studies, which revealed two key factors that affect device performance: 1) the ratio of the pressure wavelength to the channel width (𝜆𝑝/𝑊) and 2) the ratio of the shear evanescent wavelength to the channel height (𝜆𝑠/𝐻). To validate our hypothesis, we fabricated a microfluidic QCM (μ-QCM) and demonstrate a remarkable 10-fold improvement in its dissipation when compared to conventional QCM. The novel microfluidic approach offers several additional advantages, such as direct data interpretation, reduced volume requirement for sample liquids, and simplified temperature control, augmenting the sensor's overall performance. By fostering increased sensitivity, accuracy, and ease of operation, our novel paradigm unlocks new possibilities for advancing gravimetric biosensing technologies.

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Electrochemical biosensors in exosome analysis; a short journey to the present and future trends in early-stage evaluation of cancers

Cited by 37 publications

References 81 publications

Angiogenic activity of mitochondria; beyond the sole bioenergetic organelle

Angiogenic activity of mitochondria; beyond the sole bioenergetic organelle

Imaging glucose metabolism to reveal tumor progression

Microfluidic QCM enables ultrahigh Q-factor: a new paradigm for in-liquid gravimetric sensing

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