Electrochemical biosensors

Ronkainen, Niina J.; Halsall, H. Brian; Heineman, William R.

doi:10.1039/b714449k

Cited by 1,638 publications

(1,022 citation statements)

References 94 publications

Supporting

Mentioning

1,007

Contrasting

Unclassified

Order By: Relevance

“…Practically, four of the sensors listed, namely glucose, lactate, urea, and glutamate, have been widely used and commercialized [90]. Recent advances were reviewed [95][96][97].…”

Section: Biosensorsmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

View full text Add to dashboard Cite

Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades. The most recent papers, patents, and reviews on immobilization strategies and application are reviewed.

show abstract

“…Practically, four of the sensors listed, namely glucose, lactate, urea, and glutamate, have been widely used and commercialized [90]. Recent advances were reviewed [95][96][97].…”

Section: Biosensorsmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

View full text Add to dashboard Cite

show abstract

“…At present, development of detection methods such as capillary electrophoresis, fluorimetry, gas and liquid chromatography, mass spectrometry, and spectrophotometry are usually consistent and perceptive, but possess limitations, expensive including time consuming and extraction, and requiring pre-concentration steps which increase the risk generation of other hazardous byproducts, and sample loss [45]. Electrochemical sensing of toxic As molecules represents a promising approach that can be used to complement already existing techniques owing to collective features such as low cost, easy instrumentation, high selective and sensitive, and significant for miniaturization [46]. Arsenic is a toxic substance, which caused various cancers and other serious diseases after long-term exposure [47].…”

Section: Introductionmentioning

confidence: 99%

Thermally stable hybrid polyarylidene(azomethine-ether)s polymers (PAAP): an ultrasensitive arsenic(III) sensor approach

Rahman

Hussein

Aly

et al. 2018

Designed Monomers and Polymers

View full text Add to dashboard Cite

A new category of thermally stable hybrid polyarylidene(azomethine-ether)s and copolyarylidene(azomethine-ether)s (PAAP) based on diarylidenecycloalkanones has been synthesized using solution polycondensation method. For potential cationic sensor development, a thin layer of PAAP onto a flat glassy carbon electrode (GCE, surface area: 0.0316 cm2) was prepared with conducting nafion (5%) coating agent to fabricate a sensitive and selective arsenic (III) [As3+] ion in short response time in neutral buffer system. The fabricated cationic sensor was measured the analytical performances such as higher sensitivity, linear dynamic range, detection limit, reproducibility, and long-term stability towards As3+ ions. The sensitivity and detection limit were calculated as 2.714 μAμM−1cm−2 and 6.8 ± 0.1 nM (SNR of 3; 3N/S) respectively from the calibration curve. This novel approach can be initiated a well-organized route of an efficient development of heavy selective arsenic sensor for hazardous pollutants in biological, environmental, and health care fields. Real sample analysis for various environmental sample was performed with PAAP-modified-GCE.

show abstract

“…This concept is here applied to an electrochemical impedance spectroscopy (EIS) transduction, a direct and label-free method for probing protein binding events such as those from Ab-Ag interactions (Guan et al, 2001;Holford et al, 2012;Ronkainen et al, 2010). It offers fast, accurate, sensitive, selective and quantitative responses, coupled with portability, low cost and minimal power requirements.…”

Section: Introductionmentioning

confidence: 99%

Disposable immunosensor using a simple method for oriented antibody immobilization for label-free real-time detection of an oxidative stress biomarker implicated in cancer diseases

Ferreira

Sales

2014

Biosensors and Bioelectronics

View full text Add to dashboard Cite

a b s t r a c tThis work proposes a novel approach for a suitable orientation of antibodies (Ab) on an immunosensing platform, applied here to the determination of 8-hydroxy-2′-deoxyguanosine (8OHdG), a biomarker of oxidative stress that has been associated to chronic diseases, such as cancer. The anti-8OHdG was bound to an amine modified gold support through its Fc region after activation of its carboxylic functions. Nonoriented approaches of Ab binding to the platform were tested in parallel, in order to show that the presented methodology favored Ab/Ag affinity and immunodetection of the antigen.The immunosensor design was evaluated by quartz-crystal microbalance with dissipation, atomic force microscopy, electrochemical impedance spectroscopy (EIS) and square-wave voltammetry. EIS was also a suitable technique to follow the analytical behavior of the device against 8OHdG. The affinity binding between 8OHdG and the antibody immobilized in the gold modified platform increased the charge transfer resistance across the electrochemical set-up. The observed behavior was linear from 0.02 to 7.0 ng/mL of 8OHdG concentrations. The interference from glucose, urea and creatinine was found negligible. An attempt of application to synthetic samples was also successfully conducted.Overall, the presented approach enabled the production of suitably oriented Abs over a gold platform by means of a much simpler process than other oriented-Ab binding approaches described in the literature, as far as we know, and was successful in terms of analytical features and sample application.

show abstract

Electrochemical biosensors

Cited by 1,638 publications

References 94 publications

Enzyme immobilization: an update

Enzyme immobilization: an update

Thermally stable hybrid polyarylidene(azomethine-ether)s polymers (PAAP): an ultrasensitive arsenic(III) sensor approach

Disposable immunosensor using a simple method for oriented antibody immobilization for label-free real-time detection of an oxidative stress biomarker implicated in cancer diseases

Contact Info

Product

Resources

About