Electrochemical characterization of a mixed lipid monolayer supported on Au(111) electrodes with implications for doxorubicin delivery

Cholesterol Levels Affect the Performance of AuNPs-Decorated Thermo-Sensitive Liposomes as Nanocarriers for Controlled Doxorubicin Delivery

“…In a previous study, we optimized the lipid vesicle composition of L-α-phosphatidylcholine-containing L by using cationic agents such as DDAB since as stabilizer and charge-inducer substance it allowed reinforcing the anchoring process of AuNPs onto the surface of L, and also provided stabilization to the vesicles [ 1 ]. Moreover, in another study, the vesicle-stabilizing effect of DDAB and cholesterol was demonstrated by the authors in L formed with the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC):DDAB in a 3:1 ratio and the optimum ratio of cholesterol, which was determined after analyzing the Langmuir isotherms of lipid monolayers containing different mole fractions of cholesterol [ 48 ]. Those results prompted us to evaluate the effects of cholesterol levels in the performance of AuNPs-anchored L composed of DPPC and DDAB as carriers for controlled delivery of D, a well-known anticancer agent.…”

“…Thus, two series of liposomal formulations with low (L1) and high (L2) cholesterol levels were prepared. The low proportion of cholesterol was established based on previous results obtained by the authors [ 1 , 48 ]. The high proportion of cholesterol was evaluated in the current study for which monolayers at the air/water interphase were prepared and Langmuir isotherms of DPPC:DDAB:cholesterol ternary monolayers were measured to define the optimum mole fraction of cholesterol to prepare the L2 series.…”

Electrochemical Biosensors Based on Membrane-Bound Enzymes in Biomimetic Configurations

“…More studies should be done in this direction in order to obtain more stable and specific biosensors for analysis in complex samples. Although sBLMs and tBLMs have shown to be very valuable as model systems for fundamental studies of cell membrane processes [9,25,29,45,48], we think that fBLMs are more adequate for biosensor development. The presence of a water cushion in the interface between the fBLM and the electrode facilitates the insertion of transmembrane proteins in the lipid bilayer and gives higher flexibility and mobility within the biomimetic construction [77,78,82].…”

“…Electrochemical impedance spectroscopy (EIS) analysis provides interesting quantitative information about the interphase. A model that considers the sBLMs systems, like a series of constant phase elements (CPE) and an ohmic resistance, was employed to simulate the electrical properties of the interphase for a better understanding of interfacial processes, such as the incorporation of a carrier into real cell membranes and drug release [29,30]. In sBLMs, each side of the bilayer is exposed to different environments.…”

Electrode-supported biomimetic membranes: An electrochemical and surface science approach for characterizing biological cell membranes

“…Raman spectroscopy is another surface specific technique that has been employed by Krysinski et al [58] to study thiolipid monolayers at gold surface. Monolayers supported at a gold electrode surface have found application in studies involving the interactions of perfluorinated compounds [59] and drugs, such as doxorubicin with model membranes [60,61]. In addition, thiolipid coated gold nanoparticles are used as drug delivery systems [62].…”