Electrochemical Characterization of Tacrine, an Antialzheimer's Disease Drug, and Its Determination in Pharmaceuticals

Abstract

Tacrine, 9-amino-1,2,3,4-tetrahydroacridine, an antialzheimer's disease agent was electrochemically studied and a polarographic method for its quantitative determination in pharmaceuticals was developed. Tacrine was electrochemically reduced and oxidized in aqueous medium, but for analytical purposes the cathodic behavior was prefered. According to the linear relation between the peak current and the tacrine concentration a differential pulse polarographic method was developed. Precision and accuracy of the developed method was checked by recovery studies. The proposed method was applied to the individual capsule assay (ICA) of the commercial capsules, showing that the uniformity content requirements were ful®lled. Alternatively a UV spectroscopic characterization was conducted, from the pH dependence of the absorbance bands an apparent pK a of 10.44 was obtained. For comparative purposes a spectrophotometric determination also was developed.

Electrochemical Determination of Activity of Acetylcholinesterase Immobilized on Magnetic Particles

“…It is caused by saturation of the enzyme by inhibitor. We calculated LOD for this method to 8.1 μM of tacrine which is comparable to Bollo et al who achieved LOD 4.8 μM of tacrine [26].…”

Electrochemical study of fluvastatin sodium – analytical application to pharmaceutical dosage forms, human serum, and simulated gastric juice

“…Modern computer-based electrochemical instrumentation has increased the usefulness of electrochemical methods in pharmaceutical analysis and several electrochemical methods have been developed for the determination of drugs in pharmaceutical dosage forms and biological fluids [7,8,9,10,11,12,13,14,15]. The advance in experimental electrochemical techniques in the field of drug analysis is because of their simplicity, low cost, and relatively short analysis times when compared with other techniques.…”

Copper(II) complexation of tacrine hybrids with potential anti-neurodegenerative roles

“…Also, both log K 1 (ca 8-8.7) and log K 2 (6.2-6.5) values herein determined are significantly lower than, respectively, the protonation constants reported in the literature for the NH 2 group of tacrine (10.44 [24] or 9.94 [25] in water) and of 4-aminopyridine (9.14 [26]) or for the NH 2 group of cysteine (10.2 [27]). This simultaneous decrease in both values of the protonation constants can be explained on the basis of the stabilization of the deprotonated forms by formation of bifurcated hydrogen bonding involving the vicinal amide nitrogen atom as well as the tacrine nitrogen and the amine NH 2 .…”