Electrochemical Conjugation of Aptamers on a Carbon Fiber Microelectrode Enables Highly Stable and Selective In Vivo Neurosensing

Li, Xin; Jia, Ying; Zhu, Fenghui; Liu, Ran; Jiang, Yan; Jiang, Ying; Mao, Lanqun

doi:10.1002/ange.202208121

Cited by 8 publications

(6 citation statements)

References 68 publications

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“…Recently, they further used catechol as a conjunction layer to covalently link aptamers on CFE and successfully monitored DA dynamics in striatum under three consecutive stimulations of KCl (Figure 1e). 25 At the same time, the sensor shows high stability after 12 h of implantation.…”

Section: Rationally Designing the Microelectrode Interface To Achieve...mentioning

confidence: 90%

Biocompatible Microelectrode for In Vivo Sensing with Improved Performance

et al. 2023

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In vivo sensing based on implantable microelectrodes has been widely used to monitor neurochemicals due to its high spatial and temporal resolution and engineering interface designability, which has become a powerful drive to decode the mysteries of degenerative diseases and regulate neural activity. Over the past few decades, with the development of a variety of advanced materials and technologies, encouraging progress has been made in quantifying various neurochemical transients. However, because of the complex chemical atmosphere including thousands of small and large biomolecules and the inherent low mechanical property of brain tissue, the design of a compatible microelectrode for the in vivo electrochemical tracking of neurochemicals with high selectivity and stability still faces great challenges. This Perspective presents a brief account of recent representative progress in the rational regulation of the microelectrode interface to resolve the questions of selectivity and sensitive decrease resulting from antiprotein adsorption, and how to decrease the mechanical mismatch of an implanted electrode with that of brain tissue. Possible future research directions on further addressing the above key issues and a more biocompatible microelectrode for in vivo long-time electrochemical analysis are also discussed.

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Section: Rationally Designing the Microelectrode Interface To Achieve...mentioning

confidence: 90%

Biocompatible Microelectrode for In Vivo Sensing with Improved Performance

et al. 2023

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“…65,66 Recently, a dopamine aptamer was coupled with carbon-fiber microelectrodes noncovalently or using an electrochemical conjugation strategy for dopamine sensing in vivo using cyclic voltammetry. 67,68 We envision that next-generation neuroprobes will need to be soft and multimodal (i.e., sense multiple neurotransmitters and electrophysiological signals, produce optical or electrical stimulation, and drug delivery all on the same device) and have long-term recording capabilities in vivo.…”

Section: ■ Conclusionmentioning

confidence: 99%

Flexible and Implantable Polyimide Aptamer-Field-Effect Transistor Biosensors

et al. 2022

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Monitoring neurochemical signaling across time scales is critical to understanding how brains encode and store information. Flexible (vs stiff) devices have been shown to improve in vivo monitoring, particularly over longer times, by reducing tissue damage and immunological responses. Here, we report our initial steps toward developing flexible and implantable neuroprobes with aptamer-field-effect transistor (FET) biosensors for neurotransmitter monitoring. A high-throughput process was developed to fabricate thin, flexible polyimide probes using microelectromechanical-system (MEMS) technologies, where 150 flexible probes were fabricated on each 4 in. Si wafer. Probes were 150 μm wide and 7 μm thick with two FETs per tip. The bending stiffness was 1.2 × 10 −11 N•m 2 . Semiconductor thin films (3 nm In 2 O 3 ) were functionalized with DNA aptamers for target recognition, which produces aptamer conformational rearrangements detected via changes in FET conductance. Flexible aptamer-FET neuroprobes detected serotonin at femtomolar concentrations in high-ionic strength artificial cerebrospinal fluid. A straightforward implantation process was developed, where microfabricated Si carrier devices assisted with implantation such that flexible neuroprobes detected physiological relevant serotonin in a tissue-hydrogel brain mimic.

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“…Decoding the spatiotemporal dynamics of chemical neurotransmission is crucial to fully understanding the molecular mechanisms underlying various physiological processes and neurological disorders. − Neurochemical sensing based on implantable sensors has been proven to be a useful tool for in vivo monitoring neurochemical dynamics in real time. − In addition to amperometric sensors, recent studies demonstrate that potentiometric sensors emerge as a powerful electrochemical technique in terms of good compatibility to neurons, high selectivity, and less decrease in potential response resulting from the nonspecific protein adsorption because, unlike that of the amperometric sensors, − the sensitivity of potentiometric sensors is less independent of electrode area. − Typically, potentiometric sensors (i.e., ion-selective electrodes) fabricated with glass capillary have been used for in vivo monitoring of the dynamics of extracellular ions such as H + , Ca 2+ , K + , and Na + . To improve the spatial and temporal resolutions, a series of solid-state ion-selective microelectrodes based on carbon fiber electrodes (CFEs) have been prepared. , However, this mechanism could not be extended for in vivo sensing of molecules because of the lack of molecule-selective membranes to produce and output the potential signal for molecules.…”

Section: Introductionmentioning

confidence: 99%

Stability Enhancement of Galvanic Redox Potentiometry by Optimizing the Redox Couple in Counterpart Poles

Wei

Xue

et al. 2023

Anal. Chem.

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Potentiometry based on the galvanic cell mechanism, i.e., galvanic redox potentiometry (GRP), has recently emerged as a new tool for in vivo neurochemical sensing with high neuronal compatibility and good sensing property. However, the stability of open circuit voltage (E OC) outputting remains to be further improved for in vivo sensing application. In this study, we find that the E OC stability could be enhanced by adjusting the sort and the concentration ratio of the redox couple in the counterpart pole (i.e., indicating electrode) of GRP. With dopamine (DA) as the sensing target, we construct a spontaneously powered single-electrode-based GRP sensor (GRP2.0) and investigate the correlation between the stability and the redox couple used in the counterpart pole. Theoretical consideration suggests that the E OC drift is minimum when the concentration ratio of the oxidized form (O1) to the reduced form (R1) of the redox species in the backfilled solution is 1:1. The experimental results demonstrate that, compared with other redox species (i.e., dissolved O2 at 3 M KCl, potassium ferricyanide (K3Fe(CN)6), and hexaammineruthenium(III) chloride (Ru(NH3)6Cl3)) used as the counterpart pole, potassium hexachloroiridate(IV) (K2IrCl6) exhibits better chemical stability and outputs more stable E OC. As a result, when IrCl6 2–/3– with the concentration ratio of 1:1 is used as the counterpart, GRP2.0 displays not only an excellent E OC stability (i.e., 3.8 mV drifting during 2200 s for in vivo recording) but also small electrode-to-electrode variation (i.e., the maximum E OC variation between four electrodes is 2.7 mV). Upon integration with the electrophysiology, GRP2.0 records a robust DA release, accompanied by a burst of neural firing, during the optical stimulation. This study paves a new avenue to stable neurochemical sensing in vivo.

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Electrochemical Conjugation of Aptamers on a Carbon Fiber Microelectrode Enables Highly Stable and Selective In Vivo Neurosensing

Cited by 8 publications

References 68 publications

Biocompatible Microelectrode for In Vivo Sensing with Improved Performance

Biocompatible Microelectrode for In Vivo Sensing with Improved Performance

Flexible and Implantable Polyimide Aptamer-Field-Effect Transistor Biosensors

Stability Enhancement of Galvanic Redox Potentiometry by Optimizing the Redox Couple in Counterpart Poles

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