Electrochemical detection of AChE-induced gold nanostructure growth on disposable chips

Au Nanoparticle-based Detection of Human Chorionic Gonadotropin Using Electrochemical Impedance Spectroscopy

“…However, these methods are time-consuming, require expensive chemicals and trained personnel. Electrochemical immunoassays are well documented for fast, sensitive, and economical detection of various biomolecules [6][7][8][9]. Recently, the application of colloidal metal nanoparticles in electrochemical biosensors has emerged as an important field of study, considering metal nanoparticles show great stability over time as well as promote and amplify signal transfer to increase sensitivity [10][11][12].…”

Nanobiosensors and Nanobioanalyses

“…6.2 , Fiorella et al ( 2011 ) demonstrated the induction of gold nanostructures on the surface of disposable electrochemical printed (DEP) gold chips using acetylcholinesterase. 6.2 , Fiorella et al ( 2011 ) demonstrated the induction of gold nanostructures on the surface of disposable electrochemical printed (DEP) gold chips using acetylcholinesterase.…”

“…A common drawback of direct amperometry analysis of AuNPs was caused by the small population in biochemical applications, limiting current to nano-ampere levels and resulting in low sensitivity of the analysis method (Quinn et al 2003 ;Jimenez et al 2003 ). Anodic stripping voltammetry does not depend on immobilization of AuNPs, but the pre-concentration step is essential for high sensitivity (Fiorella et al 2011 ;Fu et al 2005 ;. Cathodic linear voltammetry requires AuNP oxidation reaction at 1.25 V vs. Ag/AgCl in HCl electrolyte solution, then electro-deposition to the electrode surface by linear sweeping voltammetry between 1.2 and 0.00 V ( Pumera et al 2005a , b ).…”

“…Adapted fromFiorella et al ( 2011 ) Au nanostructure growth is facilitated by acetylcholinesterase (AChE), and shows physically visible growth, build up, and color change ( right diagram ).…”