Electrochemical Detection of Ascorbic Acid in Finger-Actuated Microfluidic Chip

Abstract

The traditional quantitative analysis methods of ascorbic acid (AA), which require expensive equipment, a large amount of samples and professional technicians, are usually complex and time-consuming. A low-cost and high-efficiency AA detection device is reported in this work. It integrates a three-electrode sensor module prepared by screen printing technology, and a microfluidic chip with a finger-actuated micropump peeled from the liquid-crystal display (LCD) 3D printing resin molds. The AA detection process on this device is easy to operate. On-chip detection has been demonstrated to be 2.48 times more sensitive than off-chip detection and requires only a microliter-scale sample volume, which is much smaller than that required in traditional electrochemical methods. Experiments show that the sample and buffer can be fully mixed in the microchannel, which is consistent with the numerical simulation results wherein the mixing efficiency is greater than 90%. Commercially available tablets and beverages are also tested, and the result shows the reliability and accuracy of the device, demonstrating its broad application prospects in the field of point-of-care testing (POCT).

Efficient Electrochemical Microsensor for the Simultaneous Measurement of Hydrogen Peroxide and Ascorbic Acid in Living Brains

“…During the above simultaneous measurements, negligible cross-talk was observed between the responsive channels of H 2 O 2 and AA. The detection limits were estimated to be 0.042 μM for H 2 O 2 and 0.68 μM for AA, which are superior to lots of single H 2 O 2 or AA electrochemical sensors. − …”

“…Up to now, various approaches have been proven to be effective for determination of H 2 O 2 or AA alone, including fluorescence and colorimetric sensing, − chemical and electrochemical luminescence, − surface-enhanced Raman scattering, − capillary electrophoresis, − electrochemical sensing and so on. − Thereinto, an electrochemical method has been successfully applied in in situ , real-time measurements in vivo due to its flexibility in miniaturization and advantage in simplicity and sensitivity. − Several elegant electrochemical microsensors were reported to be applicable in in vivo analysis of H 2 O 2 or AA alone. − Nevertheless, it is still a challenge to realize simultaneous determination of H 2 O 2 and AA in the brain, since H 2 O 2 and AA possess opposite redox properties. Normally, H 2 O 2 can be easily reduced on the electrode within a cathodic scan, while AA can be cushily oxidated through an anodic scan.…”

Coupled Excitation Strategy for Crack Initiation at the Adhesive Interface of Large-Sized Ultra-Thin Chips

“…A chip is an integrated circuit manufactured on the surface of a semiconductor chip, which plays an important role in physics, military applications, science and technology, the chemical industry, medicine, and other fields [1][2][3][4]. Chip peeling and transfer are widely used for the packaging and manufacturing of high-performance devices, such as CPU, DSP, LED, RFID, and MEMERY, and represent the key to enhancing the performance and reliability of electronic devices.…”

Integrated Electrochemical Dopamine Sensing with Finger Priming Pump on a Chip

“…Finger-powered micropump has been widely developed for variety of application, including operational of finger-actuated, one-way, positive micropump for pathogens detection system (Qi et al, 2022;Jo et al, 2020), finger-actuated, negative pressure chamber for electrochemical detection of ascorbic acid (Liu et al, 2022) and microreactor (Lee et al, 2022;Park et al, 2019;Whulanza et al, 2019). The lab-on-a-chip that is being developed in this study is the integration of microreactor processes such as cell culture or adsorption with the microfluidic component, i.e., finger priming pump and manual valve.…”