Electrochemical detection of carcinoembryonic antigen based on silver nanocluster/horseradish peroxidase nanocomposite as signal probe

Hu, Quan; Zuo, Chuantao; Li, Ting; Liu, Yating; Li, Mengyu; Zhong, Min; Zhang, Yuyuan; Qi, Haiyun; Yang, Minghui

doi:10.1016/j.electacta.2015.07.086

Cited by 44 publications

(16 citation statements)

References 34 publications

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“…5B) also exhibited high CEA detection specicity, similar to that from the aptamer P. The interference protein concentrations used in the specicity experiment were referred to their physiological levels. 23 Compared with the strong responses towards CEA, no obvious responses were observed towards all the interfering proteins tested, including BSA, HSA, g-globulin, AFP, and CRP, being consistent with the bioinformatic simulation results (Fig. S5 †).…”

Section: Verication Of the Selected Dna Aptamers In Aptasensor Applisupporting

confidence: 87%

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In silicopost-SELEX screening and experimental characterizations for acquisition of high affinity DNA aptamers against carcinoembryonic antigen

Wang

Cui

et al. 2019

RSC Adv.

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Section: Verication Of the Selected Dna Aptamers In Aptasensor Applisupporting

confidence: 87%

“…20 CEA aptasensors based on this aptamer with various designs have been reported. [21][22][23] We recently reported a label-free, antibodyfree, and lectin-based electrochemical sandwich CEA aptasensor. 21 A single enzyme-based amplication strategy was used in this aptasensor design and fabrication.…”

Section: Introductionmentioning

confidence: 99%

In silicopost-SELEX screening and experimental characterizations for acquisition of high affinity DNA aptamers against carcinoembryonic antigen

Wang

Cui

et al. 2019

RSC Adv.

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show abstract

“…This explains why it has been widely used in electrochemical related fields as fuel cells [6][7][8][9][10][11][12], batteries [13][14][15][16][17][18], corrosion [19][20][21][22][23], coatings [24][25][26], electrochemical sensors [27][28] and supercapacitors [29][30][31][32]. This electrochemical technique has also been used in fields that are not traditionally related to electrochemistry as biochemical assays [33][34][35][36], oncology [37][38][39] and immunology [40][41], amongst others.…”

Section: Introductionmentioning

confidence: 99%

Harmonic analysis based method for linearity assessment and noise quantification in electrochemical impedance spectroscopy measurements: Theoretical formulation and experimental validation for Tafelian systems

Giner-Sanz

Ortega

Pérez-Herranz

2016

Electrochimica Acta

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ElsevierGiner-Sanz, JJ.; Ortega Navarro, EM.; Pérez-Herranz, V. (2016). Harmonic analysis based method for linearity assessment and noise quantification in electrochemical impedance spectroscopy measurements: Theoretical formulation and experimental validation for Tafelian systems. Abstract:Electrochemical Impedance Spectroscopy (EIS) is an electrochemical measurement technique that has been applied to a broad range of applications. Three conditions must be fulfilled in order to obtain valid EIS measurements: causality, linearity and stationarity. The non fulfilment of any of these conditions may lead to distorted and biased EIS spectra. Consequently, the verification of the four fundamental conditions is mandatory before accepting any results extracted from an EIS spectrum. In this work, a harmonic analysis based method for linearity assessment and noise quantification in EIS measurements is presented, and validated both from an experimental point of view and from a theoretical point of view, for Tafelian systems. It was shown that the presented method was able to quantitatively assess the nonlinearity of the system; and to quantify and characterize the noise. Moreover, the presented method is able to determine the threshold frequency of the system above which the system does not present significant nonlinear effects even for very large perturbation amplitudes.

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“…This wide range of fields includes typically electrochemistry related fields as fuel cells [5][6][7][8][9][10][11][12], batteries [13][14][15][16][17], coatings [18][19][20], electrochemical sensors [21][22][23][24][25][26] and supercapacitors [27][28][29][30][31]. But it also includes fields not traditionally linked to electrochemistry as enzymatic kinetics [32], biochemistry [33][34][35], food quality control [36], cancer detection [37][38] and immunology [39][40], amongst others. This electrochemical technique was first introduced in wet-electrochemistry in the late sixties of last century; and then, during the seventies, it was adopted by the solid state researchers [41].…”

Section: Introductionmentioning

confidence: 99%

Application of a Montecarlo based quantitative Kramers-Kronig test for linearity assessment of EIS measurements

Giner-Sanz

Ortega

Pérez-Herranz

2016

Electrochimica Acta

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Electrochemical Impedance Spectroscopy (EIS) is a very powerful tool to study the behaviour of electrochemical systems. Three conditions must be fulfilled during EIS measurements: causality, linearity, and stability. If any of these conditions is not achieved, then the conclusions obtained from the analysis of the measured spectra may be biased, or even misguided. For this reason, the verification of the compliance of these conditions is critical before accepting any analysis performed on an experimental spectrum. In a previous work, an experimental spectrum quantitative validation technique based on Kramers-Kronig relations was presented. The validation method consists in a Kramers-Kronig (KK) validation test, by equivalent electrical circuit fitting, coupled with a Montecarlo error propagation method. The validation technique builds a consistency region for a given confidence level that allows to discriminate between the individual points of the EIS spectrum that are consistent with the four fundamental hypotheses, and the inconsistent ones. The aim of this work is to validate experimentally if the quantitative validation technique is able to detect nonlinearities. In order to achieve this goal, the EIS spectrum of a markedly nonlinear system was measured experimentally using different perturbation amplitudes. The validation technique was applied to each one of the measured spectra. The method successfully managed to identify large nonlinearities; but did not detect slight ones.

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Electrochemical detection of carcinoembryonic antigen based on silver nanocluster/horseradish peroxidase nanocomposite as signal probe

Cited by 44 publications

References 34 publications

In silicopost-SELEX screening and experimental characterizations for acquisition of high affinity DNA aptamers against carcinoembryonic antigen

In silicopost-SELEX screening and experimental characterizations for acquisition of high affinity DNA aptamers against carcinoembryonic antigen

Harmonic analysis based method for linearity assessment and noise quantification in electrochemical impedance spectroscopy measurements: Theoretical formulation and experimental validation for Tafelian systems

Application of a Montecarlo based quantitative Kramers-Kronig test for linearity assessment of EIS measurements

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