Electrochemical detection of cholesterol in human biofluid using microneedle sensor

Li, Zhanhong; Kadian, Sachin; Mishra, Rupesh Kumar; Huang, Tingting; Zhou, Chen; Liu, Shuyuan; Wang, Zifeng; Narayan, Roger J.; Zhu, Zhigang

doi:10.1039/d2tb02142k

Cited by 19 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, compared to the portable paper sensor developed by Wang et al, [26] our ChOx-PGE biosensor showcased a low limit of detection, proving its competitive performance in cholesterol detection sensitivity without the involvement of complex nanomaterial integration. Moreover, in contrast to Li et al's [28] microneedle sensor designed for skin-worn cholesterol monitoring, our ChOx-PGE biosensor demonstrates reliable performance in saliva in non-invasive way and without the involvement of costly high-tech electrode production methods. In contrast, our study does not involve the use of nanomaterials, highlighting the cost-effectiveness and simplicity of our electrodes, which do not require extensive modification steps.…”

Section: Demonstration Of Proof-of-conceptmentioning

confidence: 80%

“…With a wide linear range (1-20 mM), and robust performance in artificial interstitial fluid and skin-mimicking phantom gel, this enzymatic microneedle sensor showcases promising potential for minimally invasive and wearable cholesterol monitoring, demonstrating stability and selectivity amidst various potential interfering biomolecules. [28] The present work reports a significant advancement in the realm of biosensor technology with the introduction of practical, disposable bioelectrodes tailored for cost-effective and rapid cholesterol detection in saliva. Under this aim, our approach focuses on the fabrication of enzyme-immobilized electrodes using EDC/NHS chemistry and Nafion dip-coating.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Eksin,

Senturk,

Erdem

2024

ChemistrySelect

View full text Add to dashboard Cite

Herein we present a practical, disposable, enzyme‐based electrochemical biosensor to revolutionize a rapid and precise measurement of cholesterol level in saliva. To create a biosensor that can measure cholesterol levels, cholesterol oxidase (ChOx) was immobilized on disposable pencil graphite electrode (PGE) via EDC/NHS chemistry. This ChOx‐PGE biosensor has been characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS). Various critical experimental parameters, including nafion concentration, enzyme concentration, enzyme‐cholesterol interaction time, were optimized ensuring consistency and precision in our results and maintained constant throughout the experiments. Under optimum conditions, the detection limit was found to be 62 μM and the sensitivity of the sensor was calculated as 1112.8 Ohm mM−1 cm−2. The potential interferences such as glucose, ascorbic acid, uric acid, cysteine was selected to test the selectivity and no significant response was observed. Finally, the proposed biosensor was effectively employed to measure cholesterol level in saliva. This biosensor boasts a rapid response time of just 20 minutes and achieves a remarkable detection limit of 95 μM for salivary cholesterol, surpassing existing techniques. Our assay not only offers an innovative solution for cholesterol monitoring but also opens new horizons in point‐of‐care diagnostics with its disposable and practical design.

show abstract

Section: Demonstration Of Proof-of-conceptmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Eksin,

Senturk,

Erdem

2024

ChemistrySelect

View full text Add to dashboard Cite

show abstract

“…MN-based medical sensing technologies can be divided into two categories. The first is electrochemical biosensors; generally, solid and hollow MNs are used as electrode substrates for further modification [85][86][87][88]. The other is the direct extraction of interstitial fluid (ISF) by means of polymer MNs or hydrogel MNs.…”

Section: Dissolving Microneedles For Diagnostics and Monitoringmentioning

confidence: 99%

The Progress in the Application of Dissolving Microneedles in Biomedicine

Yu,

Zhao,

Fan

2023

Polymers

View full text Add to dashboard Cite

In recent years, microneedle technology has been widely used for the transdermal delivery of substances, showing improvements in drug delivery effects with the advantages of minimally invasive, painless, and convenient operation. With the development of nano- and electrochemical technology, different types of microneedles are increasingly being used in other biomedical fields. Recent research progress shows that dissolving microneedles have achieved remarkable results in the fields of dermatological treatment, disease diagnosis and monitoring, and vaccine delivery, and they have a wide range of application prospects in various biomedical fields, showing their great potential as a form of clinical treatment. This review mainly focuses on dissolving microneedles, summarizing the latest research progress in various biomedical fields, providing inspiration for the subsequent intelligent and commercial development of dissolving microneedles, and providing better solutions for clinical treatment.

show abstract

“…Recognized for its minimal tissue impact, swift recovery time, and patient-friendly nature, microneedle technology has attracted significant interest and has emerged as a promising avenue for accurate clinical diagnostics and health monitoring. It also demonstrates considerable potential in the detection of subcutaneous biomarkers, efficiently tracking a range of skin-underlying biomarkers such as glucose, lactate, neurotransmitters, cholesterol, glycine, and pH through a user-friendly, swift, and non-invasive approach [29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

SERS-Based Microneedle Biosensor for In Situ and Sensitive Detection of Tyrosinase

Gu,

Zhao,

et al. 2024

Biosensors

View full text Add to dashboard Cite

Tyrosinase (TYR) emerges as a key enzyme that exerts a regulatory influence on the synthesis of melanin, thereby assuming the role of a critical biomarker for the detection of melanoma. Detecting the authentic concentration of TYR in the skin remains a primary challenge. Distinguished from ex vivo detection methods, this study introduces a novel sensor platform that integrates a microneedle (MN) biosensor with surface-enhanced Raman spectroscopy (SERS) technology for the in situ detection of TYR in human skin. The platform utilized dopamine (DA)-functionalized gold nanoparticles (Au NPs) as the capturing substrate and 4-mercaptophenylboronic acid (4-MPBA)-modified silver nanoparticles (Ag NPs) acting as the SERS probe. Here, the Au NPs were functionalized with mercaptosuccinic acid (MSA) for DA capture. In the presence of TYR, DA immobilized on the MN is preferentially oxidized to dopamine quinone (DQ), a process that results in a decreased density of SERS probes on the platform. TYR concentration was detected through variations in the signal intensity emitted by the phenylboronic acid. The detection system was able to evaluate TYR concentrations within a linear range of 0.05 U/mL to 200 U/mL and showed robust anti-interference capabilities. The proposed platform, integrating MN-based in situ sensing, SERS technology, and TYR responsiveness, holds significant importance for diagnosing cutaneous melanoma.

show abstract

Electrochemical detection of cholesterol in human biofluid using microneedle sensor

Abstract: The development of a straightforward, economical, portable, and highly sensitive sensing platform for the rapid detection of cholesterol is highly desirable for the early diagnosis of several pathologic conditions. In...

Cited by 19 publications

References 54 publications

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

The Progress in the Application of Dissolving Microneedles in Biomedicine

SERS-Based Microneedle Biosensor for In Situ and Sensitive Detection of Tyrosinase

Contact Info

Product

Resources

About